Detailed information NTG0789933

Bioinformatically predicted

Overview

• Name: comYB
• Type: Machinery gene
• Locus tag: PUW74_RS01145
• Genome accession: NZ_CP118080
• Coordinates: 198663..199706 (+)
• Length: 347 a.a.
• NCBI ID: WP_223295889.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain VSI14
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 193663..204706

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PUW74_RS01135 (PUW74_01135)	-	197260..197625 (+)	366	WP_000285373.1	DUF1033 family protein	-
PUW74_RS01140 (PUW74_01140)	comYA	197798..198769 (+)	972	WP_000250805.1	competence type IV pilus ATPase ComGA	Machinery gene
PUW74_RS01145 (PUW74_01145)	comYB	198663..199706 (+)	1044	WP_223295889.1	competence type IV pilus assembly protein ComGB	Machinery gene
PUW74_RS01150 (PUW74_01150)	comGC/cglC	199703..200032 (+)	330	WP_000793380.1	competence type IV pilus major pilin ComGC	Machinery gene
PUW74_RS01155 (PUW74_01155)	comYD	200007..200420 (+)	414	WP_000244261.1	competence type IV pilus minor pilin ComGD	Machinery gene
PUW74_RS01160 (PUW74_01160)	comGE	200392..200691 (+)	300	WP_000234804.1	competence type IV pilus minor pilin ComGE	-
PUW74_RS01165 (PUW74_01165)	comGF	200645..201106 (+)	462	WP_001874812.1	competence type IV pilus minor pilin ComGF	-
PUW74_RS01170 (PUW74_01170)	comGG	201084..201455 (+)	372	WP_000601104.1	competence type IV pilus minor pilin ComGG	-
PUW74_RS01175 (PUW74_01175)	comYH	201570..202544 (+)	975	WP_001008574.1	N-6 DNA methylase	Machinery gene
PUW74_RS01180 (PUW74_01180)	-	202576..203769 (+)	1194	WP_000047534.1	acetate kinase	-
PUW74_RS01185 (PUW74_01185)	-	203921..204127 (+)	207	WP_000798241.1	helix-turn-helix transcriptional regulator	-
PUW74_RS01190 (PUW74_01190)	-	204195..204323 (+)	129	WP_014959723.1	hypothetical protein	-

Sequence

Protein

• Length: 347 a.a.
• Molecular weight: 39936.65 Da
• Isoelectric Point: 9.9002

>NTDB_id=789933 PUW74_RS01145 WP_223295889.1 198663..199706(+) (comYB) [Streptococcus agalactiae strain VSI14]
MDKWISWLKKDISVRNRHKSKKLSLKKQRKVVQLFNNLFASGFSLTDMVTFLKRSKLLSDCYTDSMNKALLEGKDLSKML
GELGFSDTVITQVALADLHGNISRSLLKIESYLANLLLVRKKVIEVATYPLILLSFLVLIMIGLRNYLMPQLGENNFATR
LITNVPNIFLLLLAVVLIFSLIFYIIQKRLSRIKVACFLTTIPLVGSYVKLYLTAYYAREWGNLLSQGIELDQIVKVMQN
QKSKLFREIGYDMEEGFLSGKAFHQKVLDYPFFLTELSLMIEYGQVKAKLGTELDIYADEKWEDFFTKLARATQLIQPVI
FIFVALIIVMIYAAMLLPMYQNMEILS

Nucleotide

• Length: 1044 bp

>NTDB_id=789933 PUW74_RS01145 WP_223295889.1 198663..199706(+) (comYB) [Streptococcus agalactiae strain VSI14]
ATAGACAAGTGGATATCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAA
ACAACGGAAAGTAGTCCAACTTTTTAATAATCTTTTTGCTAGTGGTTTTTCTTTAACTGATATGGTCACCTTCTTAAAGA
GGAGTAAGTTATTGTCTGATTGTTATACAGATAGTATGAATAAGGCATTATTAGAGGGAAAAGATTTATCAAAAATGTTA
GGAGAGTTAGGTTTTTCTGACACTGTTATCACACAGGTTGCATTAGCTGATTTGCATGGTAACATTTCAAGGAGCCTACT
AAAGATTGAGTCTTATTTAGCTAATCTTTTGTTAGTTAGAAAAAAAGTAATAGAAGTAGCTACTTACCCATTGATATTAT
TGTCTTTTCTGGTGCTAATTATGATTGGCCTTAGGAATTATTTAATGCCCCAATTAGGAGAAAATAATTTTGCAACTAGA
CTGATTACAAATGTGCCGAATATTTTCTTATTACTTTTAGCAGTTGTACTTATTTTTAGTTTAATATTTTATATTATTCA
AAAGCGATTGTCGCGCATTAAAGTAGCTTGTTTTTTAACAACAATTCCCTTAGTTGGATCATATGTTAAGCTTTATTTAA
CTGCTTACTATGCCCGTGAGTGGGGAAATTTATTAAGTCAAGGTATTGAATTGGACCAAATTGTTAAAGTAATGCAAAAT
CAGAAATCCAAACTTTTTAGGGAAATAGGATATGACATGGAAGAAGGTTTTCTATCAGGTAAAGCATTTCACCAAAAAGT
ATTAGACTATCCGTTTTTCTTAACTGAGCTTAGTTTAATGATTGAATATGGCCAAGTTAAGGCGAAATTAGGAACAGAGT
TAGATATATATGCTGATGAGAAGTGGGAGGATTTTTTTACAAAATTAGCTAGAGCGACCCAGTTAATCCAACCCGTTATT
TTTATTTTTGTAGCTCTTATCATTGTTATGATTTATGCAGCAATGCTGTTACCAATGTATCAAAATATGGAGATATTATC
ATGA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein F (T2SSF) (34-150)

• Type II secretion system (T2SS), protein F (T2SSF) (218-338)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYB	Streptococcus mutans UA140	62.17	98.271	0.611
comYB	Streptococcus mutans UA159	61.877	98.271	0.608
comYB	Streptococcus gordonii str. Challis substr. CH1	56.305	98.271	0.553
comGB/cglB	Streptococcus mitis NCTC 12261	55.224	96.542	0.533
comGB/cglB	Streptococcus mitis SK321	54.627	96.542	0.527
comGB/cglB	Streptococcus pneumoniae Rx1	53.731	96.542	0.519
comGB/cglB	Streptococcus pneumoniae D39	53.731	96.542	0.519
comGB/cglB	Streptococcus pneumoniae R6	53.731	96.542	0.519
comGB/cglB	Streptococcus pneumoniae TIGR4	53.731	96.542	0.519
comGB	Lactococcus lactis subsp. cremoris KW2	50.148	97.118	0.487