Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   PT722_RS05435 Genome accession   NZ_CP117970
Coordinates   1189996..1190271 (+) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain B-537     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1190295..1229526 1189996..1190271 flank 24


Gene organization within MGE regions


Location: 1189996..1229526
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PT722_RS05435 comGC/cglC 1189996..1190271 (+) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  PT722_RS05440 comGD 1190268..1190711 (+) 444 WP_137007137.1 competence type IV pilus minor pilin ComGD -
  PT722_RS05445 - 1190739..1191887 (-) 1149 WP_002364359.1 tyrosine-type recombinase/integrase -
  PT722_RS05450 - 1191984..1192712 (-) 729 WP_002364358.1 ion transporter -
  PT722_RS05455 - 1192746..1192820 (-) 75 Protein_1073 ImmA/IrrE family metallo-endopeptidase -
  PT722_RS05460 - 1192880..1193785 (-) 906 WP_016630909.1 DUF4352 domain-containing protein -
  PT722_RS05465 - 1193879..1194223 (-) 345 WP_002364356.1 ImmA/IrrE family metallo-endopeptidase -
  PT722_RS05470 - 1194240..1194572 (-) 333 WP_002364355.1 helix-turn-helix domain-containing protein -
  PT722_RS05475 - 1194884..1195060 (+) 177 WP_002364354.1 helix-turn-helix domain-containing protein -
  PT722_RS05480 - 1195071..1195369 (+) 299 Protein_1078 hypothetical protein -
  PT722_RS05485 - 1195408..1196133 (+) 726 WP_002364352.1 Rha family transcriptional regulator -
  PT722_RS05490 - 1196159..1196347 (-) 189 WP_002364350.1 YegP family protein -
  PT722_RS05495 - 1196402..1196611 (+) 210 WP_002399426.1 hypothetical protein -
  PT722_RS05500 - 1196648..1196986 (+) 339 WP_002364347.1 hypothetical protein -
  PT722_RS05505 - 1197271..1197825 (-) 555 WP_002357006.1 hypothetical protein -
  PT722_RS05510 - 1198045..1198362 (+) 318 WP_002357007.1 hypothetical protein -
  PT722_RS05515 - 1198355..1199089 (+) 735 WP_002364345.1 ERF family protein -
  PT722_RS05520 - 1199094..1199735 (+) 642 WP_002364344.1 putative HNHc nuclease -
  PT722_RS05525 - 1199740..1199940 (+) 201 WP_002357010.1 hypothetical protein -
  PT722_RS05530 - 1199940..1200797 (+) 858 WP_002364343.1 helix-turn-helix domain-containing protein -
  PT722_RS05535 - 1200794..1201222 (+) 429 WP_002364342.1 RusA family crossover junction endodeoxyribonuclease -
  PT722_RS05540 - 1202130..1202546 (+) 417 WP_002364339.1 ArpU family phage packaging/lysis transcriptional regulator -
  PT722_RS05550 - 1203077..1203691 (+) 615 WP_002384355.1 hypothetical protein -
  PT722_RS05555 - 1204227..1204457 (+) 231 WP_002364294.1 hypothetical protein -
  PT722_RS05560 - 1204489..1204923 (+) 435 WP_002364295.1 terminase small subunit -
  PT722_RS05565 - 1204904..1206187 (+) 1284 WP_002364296.1 PBSX family phage terminase large subunit -
  PT722_RS05570 - 1206187..1207671 (+) 1485 WP_002364297.1 phage portal protein -
  PT722_RS05575 - 1207664..1208590 (+) 927 WP_002364298.1 minor capsid protein -
  PT722_RS05580 - 1208713..1209348 (+) 636 WP_010730822.1 DUF4355 domain-containing protein -
  PT722_RS05585 - 1209361..1210257 (+) 897 WP_002363368.1 hypothetical protein -
  PT722_RS05590 - 1210328..1210660 (+) 333 WP_002363369.1 phage head-tail connector protein -
  PT722_RS05595 - 1210657..1210932 (+) 276 WP_002364301.1 hypothetical protein -
  PT722_RS05600 - 1210929..1211267 (+) 339 WP_002363372.1 HK97-gp10 family putative phage morphogenesis protein -
  PT722_RS05605 gp17 1211264..1211656 (+) 393 WP_002363373.1 tail completion protein gp17 -
  PT722_RS05610 - 1211675..1212280 (+) 606 WP_002364302.1 phage major tail protein, TP901-1 family -
  PT722_RS05615 - 1212283..1212591 (+) 309 WP_002364303.1 hypothetical protein -
  PT722_RS05620 - 1212654..1213052 (+) 399 WP_002364304.1 tail assembly chaperone -
  PT722_RS05625 - 1213121..1213426 (+) 306 WP_002364305.1 hypothetical protein -
  PT722_RS05630 - 1213413..1217867 (+) 4455 WP_002399422.1 phage tail tape measure protein -
  PT722_RS05635 - 1217864..1218586 (+) 723 WP_002399421.1 hypothetical protein -
  PT722_RS05640 - 1218586..1220031 (+) 1446 WP_002399420.1 phage tail spike protein -
  PT722_RS05645 - 1220037..1220777 (+) 741 WP_002399419.1 hypothetical protein -
  PT722_RS05650 - 1220794..1221246 (+) 453 WP_002399418.1 hypothetical protein -
  PT722_RS05655 - 1221265..1221660 (+) 396 WP_002399417.1 hypothetical protein -
  PT722_RS05660 - 1221662..1221784 (+) 123 WP_002368228.1 XkdX family protein -
  PT722_RS05665 - 1221795..1222175 (+) 381 WP_002378446.1 phage holin family protein -
  PT722_RS05670 - 1222188..1223447 (+) 1260 WP_002365833.1 LysM peptidoglycan-binding domain-containing protein -
  PT722_RS05675 - 1224283..1224483 (+) 201 WP_002357053.1 cold-shock protein -
  PT722_RS05680 - 1224555..1224806 (+) 252 WP_002365832.1 hypothetical protein -
  PT722_RS05690 hemH 1225549..1226490 (+) 942 WP_002365831.1 ferrochelatase -
  PT722_RS05695 - 1227242..1227613 (+) 372 WP_002365830.1 type II secretion system protein -
  PT722_RS05700 comGF 1227603..1228037 (+) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  PT722_RS05705 comGG 1228037..1228390 (+) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  PT722_RS05710 - 1228519..1229526 (+) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=788660 PT722_RS05435 WP_002356991.1 1189996..1190271(+) (comGC/cglC) [Enterococcus faecalis strain B-537]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=788660 PT722_RS05435 WP_002356991.1 1189996..1190271(+) (comGC/cglC) [Enterococcus faecalis strain B-537]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATTGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396