Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   PSC76_RS08730 Genome accession   NZ_CP117496
Coordinates   1761162..1761437 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain CQHZJ21-185     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1725188..1779543 1761162..1761437 within 0


Gene organization within MGE regions


Location: 1725188..1779543
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PSC76_RS08490 (PSC76_08485) - 1725188..1726195 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  PSC76_RS08495 (PSC76_08490) comGG 1726324..1726677 (-) 354 WP_002357061.1 competence type IV pilus minor pilin ComGG -
  PSC76_RS08500 (PSC76_08495) comGF 1726677..1727111 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  PSC76_RS08505 (PSC76_08500) - 1727101..1727427 (-) 327 WP_002370967.1 type II secretion system protein -
  PSC76_RS08510 (PSC76_08505) - 1727617..1728987 (-) 1371 WP_033779878.1 DUF6056 family protein -
  PSC76_RS08515 (PSC76_08510) - 1729150..1730253 (-) 1104 WP_002395768.1 SH3 domain-containing protein -
  PSC76_RS08520 (PSC76_08515) - 1730295..1730501 (-) 207 WP_002395769.1 BhlA/UviB family holin-like peptide -
  PSC76_RS08525 (PSC76_08520) - 1730510..1730632 (-) 123 WP_002395770.1 XkdX family protein -
  PSC76_RS08530 (PSC76_08525) - 1730632..1731006 (-) 375 WP_338468810.1 hypothetical protein -
  PSC76_RS08535 (PSC76_08530) - 1731021..1731572 (-) 552 WP_338468811.1 hypothetical protein -
  PSC76_RS08540 (PSC76_08535) - 1731572..1731859 (-) 288 WP_002365086.1 collagen-like protein -
  PSC76_RS08545 (PSC76_08540) - 1731856..1732452 (-) 597 WP_002365087.1 hypothetical protein -
  PSC76_RS08550 (PSC76_08545) - 1732445..1733326 (-) 882 WP_338468812.1 phage baseplate upper protein -
  PSC76_RS08555 (PSC76_08550) - 1733345..1736140 (-) 2796 WP_338468813.1 phage tail tip lysozyme -
  PSC76_RS08560 (PSC76_08555) - 1736137..1736850 (-) 714 WP_338468814.1 phage tail protein -
  PSC76_RS08565 (PSC76_08560) - 1736847..1739150 (-) 2304 WP_338468815.1 phage tail tape measure protein -
  PSC76_RS08570 (PSC76_08565) - 1739342..1739797 (-) 456 WP_002373982.1 hypothetical protein -
  PSC76_RS08575 (PSC76_08570) - 1739797..1740444 (-) 648 WP_231433059.1 major tail protein -
  PSC76_RS08580 (PSC76_08575) - 1740441..1740803 (-) 363 WP_104858198.1 HK97-gp10 family putative phage morphogenesis protein -
  PSC76_RS08585 (PSC76_08580) - 1740793..1741131 (-) 339 WP_016617774.1 hypothetical protein -
  PSC76_RS08590 (PSC76_08585) - 1741121..1741447 (-) 327 WP_002373974.1 phage head closure protein -
  PSC76_RS08595 (PSC76_08590) - 1741425..1741706 (-) 282 WP_002395304.1 phage gp6-like head-tail connector protein -
  PSC76_RS08600 (PSC76_08595) - 1741709..1743070 (-) 1362 WP_105194770.1 phage major capsid protein -
  PSC76_RS08605 (PSC76_08600) - 1743083..1743655 (-) 573 WP_002393031.1 HK97 family phage prohead protease -
  PSC76_RS08610 (PSC76_08605) - 1743642..1744853 (-) 1212 WP_029736326.1 phage portal protein -
  PSC76_RS08615 (PSC76_08610) - 1744872..1746599 (-) 1728 WP_105194769.1 terminase TerL endonuclease subunit -
  PSC76_RS08620 (PSC76_08615) - 1746596..1747048 (-) 453 WP_064687382.1 P27 family phage terminase small subunit -
  PSC76_RS08625 (PSC76_08620) - 1747161..1747529 (-) 369 WP_002393035.1 HNH endonuclease signature motif containing protein -
  PSC76_RS08630 (PSC76_08625) - 1747529..1747909 (-) 381 WP_338468816.1 Rho termination factor N-terminal domain-containing protein -
  PSC76_RS08635 (PSC76_08630) - 1748041..1748457 (-) 417 WP_010826705.1 sigma-70 family RNA polymerase sigma factor -
  PSC76_RS08640 (PSC76_08635) - 1748763..1750616 (-) 1854 WP_338468817.1 phage/plasmid primase, P4 family -
  PSC76_RS08645 (PSC76_08640) - 1750684..1750914 (+) 231 WP_338468818.1 hypothetical protein -
  PSC76_RS08650 (PSC76_08645) - 1750911..1752473 (-) 1563 WP_338468819.1 hypothetical protein -
  PSC76_RS08655 (PSC76_08650) - 1752496..1753008 (-) 513 WP_016626786.1 hypothetical protein -
  PSC76_RS08660 (PSC76_08655) - 1753059..1753979 (-) 921 WP_338468820.1 AAA family ATPase -
  PSC76_RS08665 (PSC76_08660) - 1753976..1754194 (-) 219 WP_016634639.1 hypothetical protein -
  PSC76_RS08670 (PSC76_08665) - 1754196..1755107 (-) 912 WP_338468821.1 YqaJ viral recombinase family protein -
  PSC76_RS08675 (PSC76_08670) - 1755120..1755362 (-) 243 WP_010826713.1 hypothetical protein -
  PSC76_RS08680 (PSC76_08675) - 1755364..1756560 (-) 1197 WP_338468893.1 DEAD/DEAH box helicase -
  PSC76_RS08685 (PSC76_08680) - 1756550..1756843 (-) 294 WP_338468822.1 VRR-NUC domain-containing protein -
  PSC76_RS08690 (PSC76_08685) - 1756840..1757040 (-) 201 WP_338468823.1 hypothetical protein -
  PSC76_RS08695 (PSC76_08690) - 1757259..1757705 (-) 447 WP_338468824.1 hypothetical protein -
  PSC76_RS08700 (PSC76_08695) - 1757948..1758259 (-) 312 WP_002381719.1 hypothetical protein -
  PSC76_RS08705 (PSC76_08700) - 1758270..1758446 (-) 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
  PSC76_RS08710 (PSC76_08705) - 1758757..1759080 (+) 324 WP_338468825.1 helix-turn-helix transcriptional regulator -
  PSC76_RS08715 (PSC76_08710) - 1759098..1759442 (+) 345 WP_338468826.1 ImmA/IrrE family metallo-endopeptidase -
  PSC76_RS08720 (PSC76_08715) - 1759546..1760694 (+) 1149 WP_002387264.1 site-specific integrase -
  PSC76_RS08725 (PSC76_08720) comGD 1760731..1761165 (-) 435 Protein_1687 competence type IV pilus minor pilin ComGD -
  PSC76_RS08730 (PSC76_08725) comGC/cglC 1761162..1761437 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  PSC76_RS08735 (PSC76_08730) comGB 1761437..1762483 (-) 1047 WP_002369171.1 competence type IV pilus assembly protein ComGB -
  PSC76_RS08740 (PSC76_08735) comGA 1762440..1763408 (-) 969 WP_010717651.1 competence type IV pilus ATPase ComGA -
  PSC76_RS08745 (PSC76_08740) - 1763648..1764976 (-) 1329 WP_002362058.1 amino acid permease -
  PSC76_RS08750 (PSC76_08745) rlmN 1765266..1766339 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PSC76_RS08755 (PSC76_08750) - 1766465..1768294 (-) 1830 WP_010717652.1 ABC transporter permease -
  PSC76_RS08760 (PSC76_08755) - 1768284..1769033 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  PSC76_RS08765 (PSC76_08760) - 1769151..1769852 (-) 702 WP_002364244.1 GntR family transcriptional regulator -
  PSC76_RS08770 (PSC76_08765) - 1769983..1772406 (-) 2424 WP_002380448.1 DNA translocase FtsK -
  PSC76_RS08775 (PSC76_08770) - 1772720..1773931 (-) 1212 WP_002360017.1 NAD(P)/FAD-dependent oxidoreductase -
  PSC76_RS08780 (PSC76_08775) - 1773960..1774865 (-) 906 WP_002360016.1 prenyltransferase -
  PSC76_RS08785 (PSC76_08780) - 1774987..1775967 (+) 981 WP_002360015.1 polyprenyl synthetase family protein -
  PSC76_RS08790 (PSC76_08785) cydC 1776047..1777813 (-) 1767 WP_002364368.1 thiol reductant ABC exporter subunit CydC -
  PSC76_RS08795 (PSC76_08790) cydD 1777810..1779543 (-) 1734 WP_002364369.1 thiol reductant ABC exporter subunit CydD -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=786039 PSC76_RS08730 WP_002356991.1 1761162..1761437(-) (comGC/cglC) [Enterococcus faecalis strain CQHZJ21-185]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=786039 PSC76_RS08730 WP_002356991.1 1761162..1761437(-) (comGC/cglC) [Enterococcus faecalis strain CQHZJ21-185]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCTTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396