Detailed information NTG0785444

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PSH92_RS10745
• Genome accession: NZ_CP117451
• Coordinates: 2456783..2457901 (+)
• Length: 372 a.a.
• NCBI ID: WP_122568414.1
• Uniprot ID: -
• Organism: Pseudomonas beijingensis strain FP2034
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2451783..2462901

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PSH92_RS10730 (PSH92_10725)	-	2452740..2453654 (+)	915	WP_305470508.1	sugar kinase	-
PSH92_RS10735 (PSH92_10730)	-	2453789..2454064 (-)	276	WP_305470509.1	peptidylprolyl isomerase	-
PSH92_RS10740 (PSH92_10735)	-	2454113..2456650 (-)	2538	WP_305470510.1	PAS domain-containing sensor histidine kinase	-
PSH92_RS10745 (PSH92_10740)	pilU	2456783..2457901 (+)	1119	WP_122568414.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PSH92_RS10750 (PSH92_10745)	-	2458075..2460030 (-)	1956	WP_305470511.1	acetoacetate--CoA ligase	-
PSH92_RS10755 (PSH92_10750)	-	2460184..2460960 (-)	777	WP_305470512.1	3-hydroxybutyrate dehydrogenase	-
PSH92_RS10760 (PSH92_10755)	-	2460969..2462360 (-)	1392	WP_305470513.1	GntP family permease	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41333.26 Da
• Isoelectric Point: 6.0695

>NTDB_id=785444 PSH92_RS10745 WP_122568414.1 2456783..2457901(+) (pilU) [Pseudomonas beijingensis strain FP2034]
MEIDPLLRILASQNGSDLYMSTGAPPCARFEGVLKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNVFKQRNDVSMVIRNVKLDIPRFEDLKLPRVLLDSIMEKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTRAGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDQAL
FNLVVEGAIDEEEALKNADSANNLRLRLKLHSESGTAAPPADPAAGEWGLMD

Nucleotide

• Length: 1119 bp

>NTDB_id=785444 PSH92_RS10745 WP_122568414.1 2456783..2457901(+) (pilU) [Pseudomonas beijingensis strain FP2034]
ATGGAAATCGATCCGTTATTGCGAATCCTGGCGAGCCAAAATGGTTCCGACCTTTACATGTCCACGGGGGCGCCGCCGTG
CGCGCGTTTCGAGGGCGTGCTCAAGCCCTTGGGTAACGAAGCCTTCAAGGTCGGCGACATTGCCCGGCTCGCCGAGTCTT
TGATGGACGCCGAACAGCGGCTTGAGTTCGATCGGGAATTGGAAATGAACCTGGCGATCTCCCTGGCCGGCGTCGGGCGG
TTCCGGGTCAATGTCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCGCTTCGA
AGACCTGAAACTGCCCCGGGTGCTGTTGGACAGCATCATGGAGAAACAGGGGTTGATGCTGTTCGTCGGTGCGACCGGCT
CAGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAACCAACGGGAGGTCGGTGTCGACACCCGCAGTTTTCATGC
GGCCCTGAAAAACACCCTGCGCCAGGCGCCGGATGTGGTGCTGATCGGCGAGATCCGCGATCGGGAAACCATGGAGCATG
CCCTGGCGTTCGCCGATACCGGGCACCTGGTGATTTCCACCTTGCATGCCCATAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTCTTCCCGGAAGAGCGCCGGCCGCAGCTGCTCAACGACCTGGGCAACAACCTCAAGGCGTTCGTTTCCCAACG
CCTGGTGCGCACCCGTGCCGGCCAGCGGCGGGCGGCGGTGGAGGTGATGCTGGGCTCACCGACGGTGGCCGACCTGATCC
GGCGCAACGAACTTGGCGAGCTCAAGGGCATCATGGAGAAGTCAGAGGAACTGGGAATGCAAACCTTTGACCAGGCCCTG
TTCAACCTGGTGGTCGAAGGCGCCATCGATGAGGAGGAAGCCCTGAAAAACGCCGACTCGGCGAACAACCTGCGGTTGCG
TTTGAAGTTGCATAGCGAGTCGGGGACTGCGGCGCCACCGGCCGATCCGGCGGCGGGGGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (80-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.901	95.43	0.543
pilU	Vibrio cholerae strain A1552	53.143	94.086	0.5
pilU	Acinetobacter baylyi ADP1	51.966	95.699	0.497
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.933	100	0.392
pilT	Vibrio cholerae strain A1552	41.141	89.516	0.368
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.516	0.368
pilT	Pseudomonas stutzeri DSM 10701	39.244	92.473	0.363
pilT	Acinetobacter nosocomialis M2	40.12	89.785	0.36
pilT	Acinetobacter baumannii D1279779	40.12	89.785	0.36
pilT	Acinetobacter baumannii strain A118	40.12	89.785	0.36
pilT	Pseudomonas aeruginosa PAK	38.953	92.473	0.36