Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYD   Type   Machinery gene
Locus tag   POF62_RS01150 Genome accession   NZ_CP116707
Coordinates   199418..199831 (+) Length   137 a.a.
NCBI ID   WP_000244261.1    Uniprot ID   -
Organism   Streptococcus agalactiae strain A909 sCas9revertant     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 194418..204831
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF62_RS01130 (POF62_01130) - 196671..197036 (+) 366 WP_000285373.1 DUF1033 family protein -
  POF62_RS01135 (POF62_01135) comYA 197209..198180 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  POF62_RS01140 (POF62_01140) comYB 198074..199117 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  POF62_RS01145 (POF62_01145) comGC/cglC 199114..199443 (+) 330 WP_000793380.1 competence type IV pilus major pilin ComGC Machinery gene
  POF62_RS01150 (POF62_01150) comYD 199418..199831 (+) 414 WP_000244261.1 competence type IV pilus minor pilin ComGD Machinery gene
  POF62_RS01155 (POF62_01155) comGE 199803..200102 (+) 300 WP_000234804.1 competence type IV pilus minor pilin ComGE -
  POF62_RS01160 (POF62_01160) comGF 200056..200517 (+) 462 WP_001874812.1 competence type IV pilus minor pilin ComGF -
  POF62_RS01165 (POF62_01165) comGG 200495..200866 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  POF62_RS01170 (POF62_01170) comYH 200981..201955 (+) 975 WP_001008574.1 N-6 DNA methylase Machinery gene
  POF62_RS01175 (POF62_01175) - 201987..203180 (+) 1194 WP_000047534.1 acetate kinase -
  POF62_RS01180 (POF62_01180) - 203332..203538 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  POF62_RS01185 (POF62_01185) - 203597..203734 (+) 138 WP_001865900.1 hypothetical protein -
  POF62_RS01190 (POF62_01190) - 203775..204230 (+) 456 WP_000905673.1 hypothetical protein -

Sequence

Protein


Download         Length: 137 a.a.        Molecular weight: 15968.43 Da        Isoelectric Point: 9.9787

>NTDB_id=779247 POF62_RS01150 WP_000244261.1 199418..199831(+) (comYD) [Streptococcus agalactiae strain A909 sCas9revertant]
MVLRKFQVKAFTVLESLIVLSVVAFMTLVFSTSFNNIFRQVEETIFFISFEHLYRDTQKLSAFGQKKQTLTISHNYLENT
YERLYLPKTVKVVKSDTLAFDTNGGNSSLAKIQFECYRKTVTYQLYIGSGNYRKKEN

Nucleotide


Download         Length: 414 bp        

>NTDB_id=779247 POF62_RS01150 WP_000244261.1 199418..199831(+) (comYD) [Streptococcus agalactiae strain A909 sCas9revertant]
ATGGTTCTCAGAAAATTTCAAGTTAAAGCATTTACTGTTTTGGAGAGCCTTATTGTATTATCAGTAGTGGCATTTATGAC
GTTAGTATTTTCAACATCATTTAATAATATTTTTAGGCAGGTTGAAGAAACAATTTTCTTCATATCCTTTGAACATCTTT
ATAGAGATACTCAGAAATTGAGTGCATTTGGTCAGAAGAAACAAACCCTTACAATCTCTCATAATTATCTCGAAAATACT
TATGAGAGACTTTATTTACCTAAAACTGTAAAAGTAGTCAAAAGTGATACACTTGCATTTGACACTAATGGAGGGAATTC
AAGCTTGGCAAAAATTCAATTTGAATGTTATAGAAAAACTGTCACGTATCAATTATATATAGGAAGTGGTAATTATCGTA
AGAAAGAAAATTAG

Domains


No domain identified.



Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYD Streptococcus mutans UA140

51.515

96.35

0.496
  comYD Streptococcus mutans UA159

51.515

96.35

0.496
  comYD Streptococcus gordonii str. Challis substr. CH1

47.407

98.54

0.467
  comGD/cglD Streptococcus mitis NCTC 12261

41.045

97.81

0.401
  comGD/cglD Streptococcus pneumoniae TIGR4

43.307

92.701

0.401
  comGD/cglD Streptococcus mitis SK321

43.307

92.701

0.401
  comGD/cglD Streptococcus pneumoniae Rx1

42.52

92.701

0.394
  comGD/cglD Streptococcus pneumoniae D39

42.52

92.701

0.394
  comGD/cglD Streptococcus pneumoniae R6

42.52

92.701

0.394