Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   PML83_RS09170 Genome accession   NZ_CP116575
Coordinates   1849199..1849474 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain K205-3     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1800004..1849175 1849199..1849474 flank 24


Gene organization within MGE regions


Location: 1800004..1849474
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML83_RS08840 (PML83_08840) - 1800004..1802217 (-) 2214 WP_002381746.1 RelA/SpoT family protein -
  PML83_RS08845 (PML83_08845) - 1802432..1803184 (-) 753 WP_002357078.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  PML83_RS08850 (PML83_08850) prmA 1803186..1804133 (-) 948 WP_002357075.1 50S ribosomal protein L11 methyltransferase -
  PML83_RS08855 (PML83_08855) - 1804149..1804634 (-) 486 WP_002381745.1 DUF3013 family protein -
  PML83_RS08860 (PML83_08860) - 1804591..1805268 (-) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  PML83_RS08865 (PML83_08865) - 1805397..1806674 (+) 1278 WP_002381744.1 replication-associated recombination protein A -
  PML83_RS08875 (PML83_08875) - 1807002..1807280 (+) 279 WP_002380425.1 hypothetical protein -
  PML83_RS08880 (PML83_08880) - 1807457..1807930 (+) 474 WP_202462918.1 universal stress protein -
  PML83_RS08885 (PML83_08885) - 1808042..1809229 (-) 1188 WP_002362052.1 acetate/propionate family kinase -
  PML83_RS08890 (PML83_08890) - 1809254..1810261 (-) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  PML83_RS08895 (PML83_08895) comGG 1810390..1810743 (-) 354 WP_002381743.1 competence type IV pilus minor pilin ComGG -
  PML83_RS08900 (PML83_08900) comGF 1810743..1811177 (-) 435 WP_002357060.1 competence type IV pilus minor pilin ComGF -
  PML83_RS08905 (PML83_08905) - 1811167..1811538 (-) 372 WP_002365830.1 type II secretion system protein -
  PML83_RS08910 (PML83_08910) - 1811872..1811997 (+) 126 WP_002364184.1 hypothetical protein -
  PML83_RS08915 (PML83_08915) hemH 1812294..1813235 (-) 942 WP_002365831.1 ferrochelatase -
  PML83_RS08925 (PML83_08925) - 1813978..1814229 (-) 252 WP_002365832.1 hypothetical protein -
  PML83_RS08930 (PML83_08930) - 1814301..1814501 (-) 201 WP_002357053.1 cold-shock protein -
  PML83_RS08935 (PML83_08935) - 1815337..1816596 (-) 1260 WP_010730811.1 LysM peptidoglycan-binding domain-containing protein -
  PML83_RS08940 (PML83_08940) - 1816609..1816989 (-) 381 WP_002378446.1 phage holin family protein -
  PML83_RS08945 (PML83_08945) - 1817000..1817122 (-) 123 WP_010730812.1 XkdX family protein -
  PML83_RS08950 (PML83_08950) - 1817124..1817519 (-) 396 WP_002363385.1 hypothetical protein -
  PML83_RS08955 (PML83_08955) - 1817538..1817990 (-) 453 WP_002363384.1 hypothetical protein -
  PML83_RS08960 (PML83_08960) - 1818007..1818747 (-) 741 WP_002363383.1 hypothetical protein -
  PML83_RS08965 (PML83_08965) - 1818753..1820198 (-) 1446 WP_010730813.1 phage tail spike protein -
  PML83_RS08970 (PML83_08970) - 1820198..1820920 (-) 723 WP_010730814.1 hypothetical protein -
  PML83_RS08975 (PML83_08975) - 1820917..1825371 (-) 4455 WP_010730815.1 phage tail tape measure protein -
  PML83_RS08980 (PML83_08980) - 1825358..1825663 (-) 306 WP_002366371.1 hypothetical protein -
  PML83_RS08985 (PML83_08985) - 1825732..1826130 (-) 399 WP_010730816.1 tail assembly chaperone -
  PML83_RS08990 (PML83_08990) - 1826193..1826501 (-) 309 WP_010730817.1 hypothetical protein -
  PML83_RS08995 (PML83_08995) - 1826504..1827109 (-) 606 WP_002364302.1 phage major tail protein, TP901-1 family -
  PML83_RS09000 (PML83_09000) - 1827128..1827520 (-) 393 WP_010730818.1 tail completion protein gp17 -
  PML83_RS09005 (PML83_09005) - 1827517..1827855 (-) 339 WP_010730819.1 HK97-gp10 family putative phage morphogenesis protein -
  PML83_RS09010 (PML83_09010) - 1827852..1828127 (-) 276 WP_010730820.1 hypothetical protein -
  PML83_RS09015 (PML83_09015) - 1828124..1828456 (-) 333 WP_002363369.1 phage head-tail connector protein -
  PML83_RS09020 (PML83_09020) - 1828527..1829423 (-) 897 WP_010730821.1 hypothetical protein -
  PML83_RS09025 (PML83_09025) - 1829436..1830071 (-) 636 WP_010730822.1 DUF4355 domain-containing protein -
  PML83_RS09030 (PML83_09030) - 1830194..1831120 (-) 927 WP_002364298.1 minor capsid protein -
  PML83_RS09035 (PML83_09035) - 1831113..1832597 (-) 1485 WP_010730823.1 phage portal protein -
  PML83_RS09040 (PML83_09040) - 1832597..1833880 (-) 1284 WP_002369892.1 PBSX family phage terminase large subunit -
  PML83_RS09045 (PML83_09045) - 1833861..1834295 (-) 435 WP_002364295.1 terminase small subunit -
  PML83_RS09050 (PML83_09050) - 1834327..1834557 (-) 231 Protein_1755 hypothetical protein -
  PML83_RS09055 (PML83_09055) - 1835114..1835692 (-) 579 WP_002392366.1 hypothetical protein -
  PML83_RS09060 (PML83_09060) - 1835664..1836557 (-) 894 WP_002392365.1 hypothetical protein -
  PML83_RS09070 (PML83_09070) - 1837270..1837686 (-) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  PML83_RS09075 (PML83_09075) - 1838594..1839002 (-) 409 Protein_1759 RusA family crossover junction endodeoxyribonuclease -
  PML83_RS09080 (PML83_09080) - 1838999..1839856 (-) 858 WP_002392361.1 helix-turn-helix domain-containing protein -
  PML83_RS09085 (PML83_09085) - 1839856..1840056 (-) 201 WP_002357010.1 hypothetical protein -
  PML83_RS09090 (PML83_09090) - 1840061..1840702 (-) 642 WP_002364344.1 putative HNHc nuclease -
  PML83_RS09095 (PML83_09095) - 1840707..1841441 (-) 735 WP_002364345.1 ERF family protein -
  PML83_RS09100 (PML83_09100) - 1841434..1841751 (-) 318 WP_002357007.1 hypothetical protein -
  PML83_RS09105 (PML83_09105) - 1841971..1842525 (+) 555 WP_002357006.1 hypothetical protein -
  PML83_RS09110 (PML83_09110) - 1842810..1843148 (-) 339 WP_002357003.1 hypothetical protein -
  PML83_RS09115 (PML83_09115) - 1843185..1843394 (-) 210 WP_002357002.1 hypothetical protein -
  PML83_RS09120 (PML83_09120) - 1843449..1843637 (+) 189 WP_002357001.1 YegP family protein -
  PML83_RS09125 (PML83_09125) - 1843663..1844388 (-) 726 WP_010730824.1 ORF6C domain-containing protein -
  PML83_RS09130 (PML83_09130) - 1844427..1844738 (-) 312 WP_002381719.1 hypothetical protein -
  PML83_RS14745 - 1844750..1844917 (-) 168 WP_002388204.1 hypothetical protein -
  PML83_RS09135 (PML83_09135) - 1845335..1845532 (-) 198 WP_010712611.1 hypothetical protein -
  PML83_RS09140 (PML83_09140) - 1845545..1845727 (-) 183 WP_002358110.1 hypothetical protein -
  PML83_RS09145 (PML83_09145) - 1846025..1846357 (+) 333 WP_002392358.1 helix-turn-helix domain-containing protein -
  PML83_RS09150 (PML83_09150) - 1846375..1846719 (+) 345 WP_002378467.1 ImmA/IrrE family metallo-endopeptidase -
  PML83_RS09155 (PML83_09155) - 1846755..1847483 (+) 729 WP_002378468.1 ion transporter -
  PML83_RS09160 (PML83_09160) - 1847583..1848731 (+) 1149 WP_002388210.1 tyrosine-type recombinase/integrase -
  PML83_RS09165 (PML83_09165) comGD 1848759..1849202 (-) 444 WP_025192396.1 competence type IV pilus minor pilin ComGD -
  PML83_RS09170 (PML83_09170) comGC/cglC 1849199..1849474 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=778515 PML83_RS09170 WP_002356991.1 1849199..1849474(-) (comGC/cglC) [Enterococcus faecalis strain K205-3]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=778515 PML83_RS09170 WP_002356991.1 1849199..1849474(-) (comGC/cglC) [Enterococcus faecalis strain K205-3]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396