Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   PML73_RS09290 Genome accession   NZ_CP116571
Coordinates   1850345..1850620 (-) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain K190-1     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 1842328..1904592 1850345..1850620 within 0


Gene organization within MGE regions


Location: 1842328..1904592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML73_RS09215 (PML73_09215) - 1842424..1843317 (-) 894 WP_104669585.1 recombinase RecT -
  PML73_RS09220 (PML73_09220) - 1843320..1844261 (-) 942 WP_002364219.1 YqaJ viral recombinase family protein -
  PML73_RS09225 (PML73_09225) - 1844359..1844586 (-) 228 WP_002364220.1 hypothetical protein -
  PML73_RS09230 (PML73_09230) - 1844586..1844909 (-) 324 WP_002369793.1 hypothetical protein -
  PML73_RS09235 (PML73_09235) - 1844953..1845138 (-) 186 WP_002364222.1 hypothetical protein -
  PML73_RS09240 (PML73_09240) - 1845129..1845323 (-) 195 WP_002364223.1 hypothetical protein -
  PML73_RS09245 (PML73_09245) - 1845376..1845558 (+) 183 WP_002364224.1 YegP family protein -
  PML73_RS09250 (PML73_09250) - 1845598..1846323 (-) 726 WP_271865370.1 Rha family transcriptional regulator -
  PML73_RS09255 (PML73_09255) - 1846362..1846673 (-) 312 WP_002381719.1 hypothetical protein -
  PML73_RS09260 (PML73_09260) - 1846684..1846860 (-) 177 WP_002364354.1 helix-turn-helix domain-containing protein -
  PML73_RS09265 (PML73_09265) - 1847172..1847504 (+) 333 WP_002364355.1 helix-turn-helix domain-containing protein -
  PML73_RS09270 (PML73_09270) - 1847521..1847865 (+) 345 WP_002378467.1 ImmA/IrrE family metallo-endopeptidase -
  PML73_RS09275 (PML73_09275) - 1847901..1848629 (+) 729 WP_002378468.1 ion transporter -
  PML73_RS09280 (PML73_09280) - 1848729..1849877 (+) 1149 WP_002378469.1 tyrosine-type recombinase/integrase -
  PML73_RS09285 (PML73_09285) comGD 1849914..1850348 (-) 435 Protein_1802 competence type IV pilus minor pilin ComGD -
  PML73_RS09290 (PML73_09290) comGC/cglC 1850345..1850620 (-) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  PML73_RS09295 (PML73_09295) comGB 1850620..1851666 (-) 1047 WP_002401325.1 competence type IV pilus assembly protein ComGB -
  PML73_RS09300 (PML73_09300) comGA 1851623..1852591 (-) 969 WP_002401324.1 competence type IV pilus ATPase ComGA -
  PML73_RS09305 (PML73_09305) - 1852831..1854159 (-) 1329 WP_002362058.1 APC family permease -
  PML73_RS09310 (PML73_09310) rlmN 1854449..1855522 (-) 1074 WP_002356987.1 23S rRNA (adenine(2503)-C(2))-methyltransferase RlmN -
  PML73_RS09315 (PML73_09315) - 1855647..1857476 (-) 1830 WP_002391458.1 ABC transporter permease -
  PML73_RS09320 (PML73_09320) - 1857466..1858215 (-) 750 WP_002381711.1 ABC transporter ATP-binding protein -
  PML73_RS09325 (PML73_09325) - 1858333..1859034 (-) 702 WP_002356983.1 GntR family transcriptional regulator -
  PML73_RS09330 (PML73_09330) - 1859165..1861588 (-) 2424 WP_002411391.1 DNA translocase FtsK -
  PML73_RS09335 (PML73_09335) - 1861902..1863113 (-) 1212 WP_002411392.1 NAD(P)/FAD-dependent oxidoreductase -
  PML73_RS09340 (PML73_09340) - 1863142..1864047 (-) 906 WP_271865373.1 prenyltransferase -
  PML73_RS09345 (PML73_09345) - 1864169..1865149 (+) 981 WP_002360015.1 polyprenyl synthetase family protein -
  PML73_RS09350 (PML73_09350) cydC 1865229..1866995 (-) 1767 WP_002411395.1 thiol reductant ABC exporter subunit CydC -
  PML73_RS09355 (PML73_09355) cydD 1866992..1868725 (-) 1734 WP_002364369.1 thiol reductant ABC exporter subunit CydD -
  PML73_RS09360 (PML73_09360) cydB 1868730..1869743 (-) 1014 WP_002356976.1 cytochrome d ubiquinol oxidase subunit II -
  PML73_RS09365 (PML73_09365) - 1869740..1871158 (-) 1419 WP_002356974.1 cytochrome ubiquinol oxidase subunit I -
  PML73_RS09370 (PML73_09370) - 1872155..1873018 (-) 864 WP_002411398.1 AraC family transcriptional regulator -
  PML73_RS09375 (PML73_09375) - 1873103..1875184 (-) 2082 WP_002362072.1 DNA topoisomerase III -
  PML73_RS09380 (PML73_09380) - 1875315..1875680 (-) 366 WP_002356966.1 DUF1033 family protein -
  PML73_RS09385 (PML73_09385) - 1875778..1876347 (-) 570 WP_002411399.1 TetR/AcrR family transcriptional regulator -
  PML73_RS09390 (PML73_09390) - 1876480..1876995 (+) 516 WP_002362073.1 YbhB/YbcL family Raf kinase inhibitor-like protein -
  PML73_RS09395 (PML73_09395) - 1877012..1878244 (+) 1233 WP_002369152.1 multidrug efflux MFS transporter -
  PML73_RS09400 (PML73_09400) mnmA 1878431..1879555 (-) 1125 WP_002356960.1 tRNA 2-thiouridine(34) synthase MnmA -
  PML73_RS09405 (PML73_09405) - 1879781..1880125 (-) 345 WP_002356959.1 DUF1831 domain-containing protein -
  PML73_RS09410 (PML73_09410) - 1880193..1881338 (-) 1146 WP_002366913.1 cysteine desulfurase family protein -
  PML73_RS09415 (PML73_09415) - 1881495..1882469 (-) 975 WP_010816137.1 ribose-phosphate diphosphokinase -
  PML73_RS09420 (PML73_09420) - 1882706..1883923 (-) 1218 WP_001291561.1 tyrosine-type recombinase/integrase -
  PML73_RS09425 (PML73_09425) - 1884005..1884208 (-) 204 WP_000814511.1 excisionase -
  PML73_RS09430 (PML73_09430) - 1884192..1884443 (+) 252 WP_001845478.1 hypothetical protein -
  PML73_RS09435 (PML73_09435) - 1884669..1884899 (-) 231 WP_000857133.1 helix-turn-helix domain-containing protein -
  PML73_RS09440 (PML73_09440) - 1884896..1885318 (-) 423 WP_000804885.1 sigma-70 family RNA polymerase sigma factor -
  PML73_RS09445 (PML73_09445) - 1885823..1886176 (+) 354 WP_001227347.1 helix-turn-helix transcriptional regulator -
  PML73_RS09450 (PML73_09450) - 1886236..1886403 (-) 168 WP_000336323.1 cysteine-rich KTR domain-containing protein -
  PML73_RS09455 (PML73_09455) tet(M) 1886522..1888441 (-) 1920 WP_000691749.1 tetracycline resistance ribosomal protection protein Tet(M) -
  PML73_RS15075 - 1888457..1888504 (-) 48 Protein_1837 hypothetical protein -
  PML73_RS09465 (PML73_09465) - 1888818..1889750 (-) 933 WP_001224318.1 conjugal transfer protein -
  PML73_RS09470 (PML73_09470) - 1889747..1890748 (-) 1002 WP_000769868.1 lysozyme family protein -
  PML73_RS09475 (PML73_09475) - 1890745..1892922 (-) 2178 WP_000804748.1 CD3337/EF1877 family mobilome membrane protein -
  PML73_RS09480 (PML73_09480) - 1892925..1895372 (-) 2448 WP_000331160.1 ATP-binding protein -
  PML73_RS09485 (PML73_09485) - 1895356..1895748 (-) 393 WP_000723888.1 conjugal transfer protein -
  PML73_RS09490 (PML73_09490) - 1895837..1896334 (-) 498 WP_010712901.1 antirestriction protein ArdA -
  PML73_RS09495 (PML73_09495) - 1896451..1896672 (-) 222 WP_001009056.1 hypothetical protein -
  PML73_RS09500 (PML73_09500) mobT 1896715..1897920 (-) 1206 WP_000398284.1 MobT family relaxase -
  PML73_RS09505 (PML73_09505) - 1897943..1898095 (-) 153 WP_000879507.1 hypothetical protein -
  PML73_RS09510 (PML73_09510) - 1898098..1899483 (-) 1386 WP_000813488.1 FtsK/SpoIIIE domain-containing protein -
  PML73_RS09515 (PML73_09515) - 1899512..1899898 (-) 387 WP_000985015.1 YdcP family protein -
  PML73_RS09520 (PML73_09520) - 1899914..1900228 (-) 315 WP_000420682.1 YdcP family protein -
  PML73_RS09525 (PML73_09525) - 1900720..1901469 (+) 750 WP_002360005.1 metal ABC transporter ATP-binding protein -
  PML73_RS09530 (PML73_09530) - 1901466..1902335 (+) 870 WP_002369145.1 metal ABC transporter permease -
  PML73_RS09535 (PML73_09535) - 1902322..1903248 (+) 927 WP_002356954.1 metal ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=778477 PML73_RS09290 WP_002356991.1 1850345..1850620(-) (comGC/cglC) [Enterococcus faecalis strain K190-1]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=778477 PML73_RS09290 WP_002356991.1 1850345..1850620(-) (comGC/cglC) [Enterococcus faecalis strain K190-1]
ATGAAAAAGAAACAAAAATACGCGGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTCCCTAACTTAGCGAAACATAAAGAAACAGTTGACAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396