NTDB

Overview

Name	pilU	Type	Machinery gene
Locus tag	PJ250_RS04275	Genome accession	NZ_CP116339
Coordinates	913744..914880 (+)	Length	378 a.a.
NCBI ID	WP_271647309.1	Uniprot ID	-
Organism	Pseudoxanthomonas sp. JBR18
Function	assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	889284..913645	913744..914880	flank	99

Gene organization within MGE regions

Location: 889284..914880

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PJ250_RS04170 (PJ250_04170)	-	889284..889451 (+)	168	WP_271647290.1	transposase	-
PJ250_RS04175 (PJ250_04175)	-	889886..890335 (-)	450	WP_271647291.1	hypothetical protein	-
PJ250_RS04180 (PJ250_04180)	-	890527..891855 (-)	1329	WP_271647292.1	hypothetical protein	-
PJ250_RS04185 (PJ250_04185)	-	891852..893543 (-)	1692	WP_333909502.1	DUF6311 domain-containing protein	-
PJ250_RS04190 (PJ250_04190)	-	893534..894133 (-)	600	WP_271647293.1	hypothetical protein	-
PJ250_RS04195 (PJ250_04195)	-	894130..894519 (-)	390	WP_271647294.1	GtrA family protein	-
PJ250_RS04200 (PJ250_04200)	-	894512..895468 (-)	957	WP_271647295.1	glycosyltransferase	-
PJ250_RS04205 (PJ250_04205)	-	895465..896415 (-)	951	WP_271647296.1	UDP-glucuronic acid decarboxylase family protein	-
PJ250_RS04210 (PJ250_04210)	-	896555..897457 (-)	903	WP_271647297.1	GDP-mannose 4,6-dehydratase	-
PJ250_RS04215 (PJ250_04215)	gmd	897457..898497 (-)	1041	WP_271647298.1	GDP-mannose 4,6-dehydratase	-
PJ250_RS04220 (PJ250_04220)	-	898575..901139 (-)	2565	WP_271647299.1	glycosyltransferase family 1 protein	-
PJ250_RS04225 (PJ250_04225)	-	901136..903238 (-)	2103	WP_271647300.1	methyltransferase domain-containing protein	-
PJ250_RS04230 (PJ250_04230)	-	903291..904586 (-)	1296	WP_271647301.1	ABC transporter ATP-binding protein	-
PJ250_RS04235 (PJ250_04235)	-	904583..905377 (-)	795	WP_271647302.1	ABC transporter permease	-
PJ250_RS04240 (PJ250_04240)	-	905374..908553 (-)	3180	WP_271647303.1	glycosyltransferase	-
PJ250_RS04245 (PJ250_04245)	-	908557..908802 (-)	246	WP_271647304.1	hypothetical protein	-
PJ250_RS04250 (PJ250_04250)	-	908908..910110 (-)	1203	WP_271647305.1	cystathionine gamma-synthase	-
PJ250_RS04255 (PJ250_04255)	-	910202..911572 (-)	1371	WP_271648520.1	pyridoxal-phosphate dependent enzyme	-
PJ250_RS04260 (PJ250_04260)	-	911763..911981 (-)	219	WP_271647306.1	YdcH family protein	-
PJ250_RS04265 (PJ250_04265)	-	912315..913196 (-)	882	WP_271647307.1	hypothetical protein	-
PJ250_RS04270 (PJ250_04270)	-	913193..913645 (-)	453	WP_271647308.1	DUF4398 domain-containing protein	-
PJ250_RS04275 (PJ250_04275)	pilU	913744..914880 (+)	1137	WP_271647309.1	PilT/PilU family type 4a pilus ATPase	Machinery gene

Sequence

Protein

Download Length: 378 a.a. Molecular weight: 41799.03 Da Isoelectric Point: 5.9743

                                >NTDB_id=776655 PJ250_RS04275 WP_271647309.1 913744..914880(+) (pilU) [Pseudoxanthomonas sp. JBR18]

MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAVALADAGR

FRVNVFKQRGEVGMVIRAIRSKIPSIEELQLPEVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDYRNSSTTGHILTIED

PIEYLHRHKQSIVNQREVGLDTHAFHNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI

LNFFPETAHKNVLMNLALNLRAVVSQRLVRGVDGRRLPAAEVLINTPVIRDLLRRGQIHEIKQAMEESLEDGMESFDQCL

FRMVKEGKIAQDEALRAADSRDGLALKFRLSEGAAGEHDPYADLYEQSGTSSITHGFS

Nucleotide

Download Length: 1137 bp

                                >NTDB_id=776655 PJ250_RS04275 WP_271647309.1 913744..914880(+) (pilU) [Pseudoxanthomonas sp. JBR18]

ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCCGTCTA

CATCAAGATCGAAGGCAAGCTCTACCCGCTGGGCAATACTGGCCTGCCACCGGGCATGGTCAAGAAGATCGCCTACTCGC

TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTGAACATGGCCGTGGCCCTGGCCGACGCCGGCCGC

TTCCGCGTCAACGTGTTCAAGCAGCGCGGCGAGGTCGGCATGGTGATCCGCGCCATCCGCAGCAAGATCCCCTCCATCGA

GGAACTGCAGCTGCCGGAGGTGCTCAAGGACATCATCATGACCCCGCGCGGCTTGGTCCTGCTGGTCGGCTCGACCGGTT

CGGGCAAGTCGACCTCGCTGGCCTCGATGATCGACTACCGCAACTCCAGCACCACCGGACACATCCTCACCATCGAGGAC

CCGATCGAATACCTGCACCGGCACAAGCAGTCCATCGTCAACCAGCGTGAGGTCGGCCTGGATACCCACGCCTTTCACAA

CGCGCTGAAGAACGCCATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAGATCCTGGATGCGACCACGATGGAGGCGG

CCATCGCCTTTGCCGAGACCGGCCATCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC

CTCAACTTCTTCCCGGAAACCGCCCACAAGAACGTCCTGATGAACCTGGCGCTGAACCTGCGCGCCGTGGTGTCCCAGCG

CCTGGTGCGCGGCGTGGACGGTCGGCGTTTGCCGGCGGCCGAAGTGCTGATCAACACCCCGGTGATCCGAGACCTGCTGC

GCCGTGGCCAGATCCACGAGATCAAGCAAGCCATGGAGGAGTCGCTGGAAGACGGCATGGAGTCCTTCGACCAGTGCCTG

TTCCGCATGGTCAAGGAAGGCAAGATCGCCCAGGACGAGGCGCTGCGCGCGGCGGACTCGCGCGACGGACTGGCGCTGAA

GTTCCGCCTCTCCGAAGGTGCTGCCGGCGAGCATGATCCCTATGCCGACCTCTACGAGCAGAGCGGCACCTCATCGATCA

CCCACGGCTTCAGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
pilU	Pseudomonas stutzeri DSM 10701	50.696	94.974	0.481
pilU	Acinetobacter baylyi ADP1	48.876	94.18	0.46
pilU	Vibrio cholerae strain A1552	48.87	93.651	0.458
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	43.194	100	0.437
pilT	Legionella pneumophila strain ERS1305867	41.124	89.418	0.368
pilT	Pseudomonas aeruginosa PAK	41.124	89.418	0.368
pilT	Legionella pneumophila strain Lp02	41.124	89.418	0.368
pilT	Pseudomonas stutzeri DSM 10701	40.828	89.418	0.365
pilT	Acinetobacter nosocomialis M2	40.533	89.418	0.362
pilT	Acinetobacter baumannii D1279779	40.533	89.418	0.362
pilT	Acinetobacter baumannii strain A118	40.533	89.418	0.362

Detailed information NTG0776655