Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PJ250_RS01400 Genome accession   NZ_CP116339
Coordinates   261879..263009 (-) Length   376 a.a.
NCBI ID   WP_271646778.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. JBR18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 256879..268009
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ250_RS01375 (PJ250_01375) - 257263..257430 (-) 168 WP_271646775.1 30S ribosomal protein THX -
  PJ250_RS01380 (PJ250_01380) - 257647..258054 (-) 408 WP_271646776.1 MerC domain-containing protein -
  PJ250_RS01385 (PJ250_01385) - 258159..260219 (+) 2061 WP_271648479.1 TonB-dependent receptor -
  PJ250_RS01390 (PJ250_01390) - 260416..261111 (-) 696 WP_271648480.1 YitT family protein -
  PJ250_RS01395 (PJ250_01395) - 261249..261815 (-) 567 WP_271646777.1 hypothetical protein -
  PJ250_RS01400 (PJ250_01400) pilU 261879..263009 (-) 1131 WP_271646778.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJ250_RS01405 (PJ250_01405) pilT 263105..264142 (-) 1038 WP_271646779.1 type IV pilus twitching motility protein PilT Machinery gene
  PJ250_RS01410 (PJ250_01410) - 264245..264925 (+) 681 WP_271646780.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJ250_RS01415 (PJ250_01415) proC 265076..265912 (+) 837 WP_271646781.1 pyrroline-5-carboxylate reductase -
  PJ250_RS01420 (PJ250_01420) - 265922..266389 (+) 468 WP_271646782.1 DUF4426 domain-containing protein -
  PJ250_RS01425 (PJ250_01425) - 266399..267196 (-) 798 WP_271648481.1 cobalamin-binding protein -
  PJ250_RS01430 (PJ250_01430) aac(6') 267201..267644 (-) 444 WP_271646783.1 aminoglycoside 6'-N-acetyltransferase -
  PJ250_RS01435 (PJ250_01435) - 267641..267994 (-) 354 WP_271646784.1 hypothetical protein -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41881.13 Da        Isoelectric Point: 6.8287

>NTDB_id=776652 PJ250_RS01400 WP_271646778.1 261879..263009(-) (pilU) [Pseudoxanthomonas sp. JBR18]
MSSIDFTSFLKLMSHQKASDLFITSGMPPSIKVHGKLTPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPVIKTLAMTKRGIIIFVGATGTGKSTSLAAMIGYRNQNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAISFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRSQLLMDLSLNLRGVVAQQLVPTPDGKARRVAMEIMLGTPLIQDYIREGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLASGGDARTLAQGLDGVEVAEAR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=776652 PJ250_RS01400 WP_271646778.1 261879..263009(-) (pilU) [Pseudoxanthomonas sp. JBR18]
ATGAGCAGCATCGACTTCACCTCGTTCCTCAAGCTGATGTCGCACCAGAAGGCATCGGACCTGTTCATCACCTCCGGCAT
GCCGCCGTCGATCAAGGTGCATGGCAAGCTGACCCCCATCACCCAGACCCCGCTGACCCCGCAACAGTCGCGCGATCTGG
TGCTCAACGTCATGACGCCGGCCCAGCGCGAGGAGTTCGAGAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGCCGCTTCCGCGTGAGCTGCTTCTACCAGCGCAACCAGGTTGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGCGTCGACGAGCTCAACCTGCCGCCGGTGATCAAGACCCTGGCGATGACCAAGCGCGGCATCATCATCTTTGTCGGCG
CCACCGGCACCGGCAAGTCGACCTCGCTGGCTGCGATGATCGGCTACCGCAACCAGAACTCGACCGGGCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCACGCCATCTCCTTCGCCGAAACCGGCCACCTGGTGCTGTGCACCCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAGGACCGTCGCAGCCAGCTGCTGATGGACCTGTCGCTGAACCTGCGCGGCGTGGT
CGCCCAGCAACTGGTACCGACCCCGGATGGCAAGGCGCGCCGGGTGGCCATGGAGATCATGCTCGGCACGCCGCTGATCC
AGGACTACATCCGCGAAGGCGAGATCCACAAGCTCAAGGAGGTCATGAAGGAGTCCACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTCTACCAGGCCGGCGAGATCTCCTACGAGGACGCGCTGCGCTATGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCTCCGGCGGCGACGCGCGCACCCTGGCCCAGGGGCTGGACGGGGTCGAGGTGGCCG
AGGCGCGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

64.306

93.883

0.604
  pilU Vibrio cholerae strain A1552

53.371

94.681

0.505
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.896

94.947

0.388
  pilT Acinetobacter baumannii strain A118

40.407

91.489

0.37
  pilT Acinetobacter nosocomialis M2

40.407

91.489

0.37
  pilT Acinetobacter baumannii D1279779

40.407

91.489

0.37
  pilT Legionella pneumophila strain Lp02

41.194

89.096

0.367
  pilT Legionella pneumophila strain ERS1305867

41.194

89.096

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362