Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   PJ250_RS01405 Genome accession   NZ_CP116339
Coordinates   263105..264142 (-) Length   345 a.a.
NCBI ID   WP_271646779.1    Uniprot ID   -
Organism   Pseudoxanthomonas sp. JBR18     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 258105..269142
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJ250_RS01385 (PJ250_01385) - 258159..260219 (+) 2061 WP_271648479.1 TonB-dependent receptor -
  PJ250_RS01390 (PJ250_01390) - 260416..261111 (-) 696 WP_271648480.1 YitT family protein -
  PJ250_RS01395 (PJ250_01395) - 261249..261815 (-) 567 WP_271646777.1 hypothetical protein -
  PJ250_RS01400 (PJ250_01400) pilU 261879..263009 (-) 1131 WP_271646778.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PJ250_RS01405 (PJ250_01405) pilT 263105..264142 (-) 1038 WP_271646779.1 type IV pilus twitching motility protein PilT Machinery gene
  PJ250_RS01410 (PJ250_01410) - 264245..264925 (+) 681 WP_271646780.1 YggS family pyridoxal phosphate-dependent enzyme -
  PJ250_RS01415 (PJ250_01415) proC 265076..265912 (+) 837 WP_271646781.1 pyrroline-5-carboxylate reductase -
  PJ250_RS01420 (PJ250_01420) - 265922..266389 (+) 468 WP_271646782.1 DUF4426 domain-containing protein -
  PJ250_RS01425 (PJ250_01425) - 266399..267196 (-) 798 WP_271648481.1 cobalamin-binding protein -
  PJ250_RS01430 (PJ250_01430) aac(6') 267201..267644 (-) 444 WP_271646783.1 aminoglycoside 6'-N-acetyltransferase -
  PJ250_RS01435 (PJ250_01435) - 267641..267994 (-) 354 WP_271646784.1 hypothetical protein -
  PJ250_RS01440 (PJ250_01440) pdxH 267991..268608 (-) 618 WP_271646785.1 pyridoxamine 5'-phosphate oxidase -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38697.42 Da        Isoelectric Point: 6.6654

>NTDB_id=776653 PJ250_RS01405 WP_271646779.1 263105..264142(-) (pilT) [Pseudoxanthomonas sp. JBR18]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPLFREMVQQPQGLILVTGPTGSGKSTTLAAMIDFINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRSVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQFGMMTLDQHL
QDLVKRSQITRQQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=776653 PJ250_RS01405 WP_271646779.1 263105..264142(-) (pilT) [Pseudoxanthomonas sp. JBR18]
ATGGATATCGCCGAACTGCTGGCCTTCTCGGTCAAGAACAAGGCTTCGGACCTGCACCTGTCCGCCGGGTTGCCGCCGAT
GATCCGCGTGGACGGCGATGTCCGCCGCATCAACATCCCCGCGCTGGACCACAAGCAGGTCCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGCCGCGACTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATCCCCTCGTTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGCGCGGTGTTCCGCACGATTCCGTCCGAAGTGCTGACCCTGGA
GGACCTGGGCTGCCCGCCGCTGTTCCGCGAGATGGTCCAGCAGCCGCAGGGTCTGATCCTGGTGACCGGGCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTTCATCAACAAGAACGAATACGGTCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTGCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAGGTGCACCGCGACACCCACGGCTTCAACGA
AGCGCTGCGCTCGGCGCTGCGTGAGGACCCCGACTACATCCTGGTCGGCGAGTTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGTTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGCTCGGTGATCTCCCAGGC
CCTGCTGAAGAAGGTCGGCGGCGGGCGTACCGCCGCGTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATTCAGACCGGCCAGCAGTTCGGCATGATGACCCTGGACCAGCACCTG
CAGGACCTGGTCAAGCGCAGCCAGATCACGCGCCAGCAGGCCAAGGAATACGCCAAGGACAAGCGCCTGTTCGAGTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter baumannii D1279779

79.07

99.71

0.788
  pilT Acinetobacter baumannii strain A118

79.07

99.71

0.788
  pilT Acinetobacter nosocomialis M2

79.07

99.71

0.788
  pilT Pseudomonas stutzeri DSM 10701

77.907

99.71

0.777
  pilT Acinetobacter baylyi ADP1

77.035

99.71

0.768
  pilT Pseudomonas aeruginosa PAK

78.171

98.261

0.768
  pilT Legionella pneumophila strain Lp02

74.709

99.71

0.745
  pilT Legionella pneumophila strain ERS1305867

74.709

99.71

0.745
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

69.254

97.101

0.672
  pilT Vibrio cholerae strain A1552

69.254

97.101

0.672
  pilT Neisseria meningitidis 8013

64.516

98.841

0.638
  pilT Neisseria gonorrhoeae MS11

64.223

98.841

0.635
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.275

100

0.493
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.823

98.261

0.391
  pilU Acinetobacter baylyi ADP1

39.412

98.551

0.388