Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   QUR98_RS03820 Genome accession   NZ_CP128578
Coordinates   787965..789458 (+) Length   497 a.a.
NCBI ID   WP_001530857.1    Uniprot ID   -
Organism   Escherichia coli strain VG38-53E1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 782965..794458
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QUR98_RS03800 (QUR98_03800) pilD 783613..784422 (+) 810 WP_000895878.1 prepilin peptidase PppA Machinery gene
  QUR98_RS03805 (QUR98_03805) gspS2 784488..784898 (+) 411 WP_001309757.1 type II secretion system pilot lipoprotein GspS-beta -
  QUR98_RS03810 (QUR98_03810) gspC 784916..785875 (+) 960 WP_000135091.1 type II secretion system protein GspC -
  QUR98_RS03815 (QUR98_03815) gspD 785905..787965 (+) 2061 WP_000498829.1 type II secretion system secretin GspD -
  QUR98_RS03820 (QUR98_03820) pilB/pilB1 787965..789458 (+) 1494 WP_001530857.1 type II secretion system ATPase GspE Machinery gene
  QUR98_RS03825 (QUR98_03825) gspF 789458..790681 (+) 1224 WP_021521036.1 type II secretion system inner membrane protein GspF -
  QUR98_RS03830 (QUR98_03830) gspG 790698..791153 (+) 456 WP_001087289.1 type II secretion system major pseudopilin GspG -
  QUR98_RS03835 (QUR98_03835) gspH 791157..791720 (+) 564 WP_021521035.1 type II secretion system minor pseudopilin GspH -
  QUR98_RS03840 (QUR98_03840) gspI 791717..792088 (+) 372 WP_021521034.1 type II secretion system minor pseudopilin GspI -
  QUR98_RS03845 (QUR98_03845) gspJ 792085..792690 (+) 606 WP_021521033.1 type II secretion system minor pseudopilin GspJ -
  QUR98_RS03850 (QUR98_03850) gspK 792687..793664 (+) 978 WP_021521032.1 type II secretion system minor pseudopilin GspK -

Sequence

Protein


Download         Length: 497 a.a.        Molecular weight: 55180.33 Da        Isoelectric Point: 5.9081

>NTDB_id=774434 QUR98_RS03820 WP_001530857.1 787965..789458(+) (pilB/pilB1) [Escherichia coli strain VG38-53E1]
MVPVAQETTANTVRLPYSFSRRFSLVAWCEASLEILHVHPLSLSVLQELQRGLNAPFTLRQIDEAEFEQRLNAVWQRDSS
EARQLMEDLGSAEDFFTLAEELPETEDLLESDDDAPIIKLINAMLAEAIKEGASDIHIETFEKSLVIRFRVDGTLHEMLR
PGRKLASLLVSRIKVMARLDIAEKRVPQDGRIALLLGGRAIDVRVSTMPSAWGERVVLRLLDKNQARLTLERLGLSLELT
AQLRQLLHKPHGIFLVTGPTGSGKSTTLYAGLQELNNHSRNILTVEDPIEYMIEGIGQTQVNTRVGMTFARGLRAILRQD
PDVVMVGEIRDTETAEIAVQASLTGHLVLSTLHTNTAVGAITRLQDMGVEPFLLSSSLTGVMAQRLVRTLCPDCRQSAPA
TDEEKRLLGITDARTVTLYHPQGCPACNHKGFRGRTAIHELIVVDATLRDLIHRQAGELELERYVRQHSAGIRSNGIEKV
LAGETSLDEVLRVTMEA

Nucleotide


Download         Length: 1494 bp        

>NTDB_id=774434 QUR98_RS03820 WP_001530857.1 787965..789458(+) (pilB/pilB1) [Escherichia coli strain VG38-53E1]
ATGGTGCCTGTAGCACAGGAAACCACCGCTAACACCGTGCGTCTGCCCTACAGTTTCAGCCGTCGGTTTAGCCTGGTGGC
ATGGTGCGAAGCGTCGCTGGAGATCCTCCATGTTCATCCGTTGTCGCTCTCTGTTTTGCAGGAGCTGCAGCGGGGGCTGA
ACGCGCCCTTTACGCTGCGGCAAATCGACGAAGCCGAATTTGAACAGCGGCTGAATGCGGTCTGGCAGCGGGACTCTTCC
GAGGCTCGCCAACTGATGGAAGATCTCGGTTCTGCCGAGGACTTTTTTACCCTCGCAGAAGAACTGCCGGAAACGGAAGA
TCTGCTGGAAAGTGACGACGATGCGCCGATCATCAAACTGATCAACGCCATGCTGGCAGAGGCGATTAAAGAAGGCGCTT
CGGATATCCACATTGAGACGTTTGAAAAGAGTCTGGTGATCCGTTTTCGTGTTGACGGCACATTACATGAAATGTTGCGC
CCCGGTCGTAAACTGGCCTCGCTGCTGGTCTCGCGTATCAAGGTGATGGCGCGACTGGATATCGCCGAAAAGCGCGTACC
GCAGGATGGCCGTATTGCGCTGCTGCTGGGCGGTCGGGCGATTGACGTCCGTGTATCAACCATGCCTTCCGCCTGGGGGG
AACGGGTGGTGCTGCGACTGCTGGACAAAAACCAGGCTCGCCTGACGCTGGAGCGTCTGGGTTTAAGTCTCGAACTGACT
GCGCAGTTGCGCCAGCTGTTACACAAACCGCACGGCATTTTTCTGGTGACGGGGCCGACCGGTTCCGGCAAAAGCACCAC
GCTGTACGCTGGATTGCAGGAGCTGAACAACCACTCGCGTAACATTCTCACGGTTGAAGACCCTATCGAATACATGATTG
AAGGGATCGGTCAGACGCAGGTTAACACCCGCGTCGGCATGACATTCGCCCGTGGCCTGCGCGCAATTTTGCGTCAGGAC
CCGGATGTGGTGATGGTCGGTGAAATCCGCGATACCGAAACCGCAGAAATCGCTGTTCAGGCTTCACTGACCGGACACCT
GGTACTTTCCACGCTGCATACCAACACGGCGGTGGGGGCGATCACACGTTTGCAGGATATGGGCGTGGAGCCTTTCCTGC
TCTCTTCCAGTCTGACGGGCGTGATGGCGCAGCGACTGGTTCGCACGCTGTGTCCCGATTGCCGCCAGTCCGCGCCTGCC
ACCGACGAAGAAAAACGCCTGCTGGGGATTACCGATGCGCGTACCGTCACGCTGTACCATCCGCAGGGCTGCCCCGCCTG
TAATCACAAAGGTTTTCGCGGACGTACTGCCATCCATGAACTGATTGTGGTGGACGCCACATTGCGTGATTTGATCCACC
GTCAGGCCGGGGAACTGGAGCTGGAACGTTATGTCCGGCAACACTCTGCGGGTATCCGCAGTAACGGCATTGAGAAAGTG
CTCGCCGGAGAAACCTCTCTCGATGAAGTTCTGCGGGTAACCATGGAGGCGTAA

Domains

Predicted by InterProScan.

Type II/IV secretion system protein (T2SSE)

(123-389)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

39.556

100

0.431
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

47.529

85.513

0.406
  pilB Vibrio parahaemolyticus RIMD 2210633

48.111

79.879

0.384
  pilB Legionella pneumophila strain ERS1305867

43.619

86.72

0.378
  pilB Vibrio campbellii strain DS40M4

47.222

79.678

0.376
  pilB Acinetobacter baumannii D1279779

46.366

80.282

0.372
  pilB Vibrio cholerae strain A1552

45.96

79.678

0.366
  pilB Acinetobacter baylyi ADP1

46.41

78.471

0.364
  pilF Thermus thermophilus HB27

45

80.483

0.362