Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O8413_RS09995 Genome accession   NZ_CP115188
Coordinates   2153778..2155502 (-) Length   574 a.a.
NCBI ID   WP_041942471.1    Uniprot ID   -
Organism   Vibrio furnissii strain MT14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2148778..2160502
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8413_RS09975 (O8413_09975) - 2149456..2150286 (-) 831 WP_014204340.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  O8413_RS09980 (O8413_09980) - 2150381..2151913 (-) 1533 WP_283512489.1 AAA family ATPase -
  O8413_RS09985 (O8413_09985) aroB 2151959..2153044 (-) 1086 WP_154180460.1 3-dehydroquinate synthase -
  O8413_RS09990 (O8413_09990) aroK 2153072..2153590 (-) 519 WP_004728569.1 shikimate kinase AroK -
  O8413_RS09995 (O8413_09995) pilQ 2153778..2155502 (-) 1725 WP_041942471.1 type IV pilus secretin PilQ family protein Machinery gene
  O8413_RS10000 (O8413_10000) pilP 2155527..2156039 (-) 513 WP_143680276.1 pilus assembly protein PilP Machinery gene
  O8413_RS10005 (O8413_10005) pilO 2156029..2156619 (-) 591 WP_158141364.1 type 4a pilus biogenesis protein PilO Machinery gene
  O8413_RS10010 (O8413_10010) pilN 2156612..2157202 (-) 591 WP_158165891.1 PilN domain-containing protein Machinery gene
  O8413_RS10015 (O8413_10015) pilM 2157192..2158196 (-) 1005 WP_283512490.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 574 a.a.        Molecular weight: 61962.64 Da        Isoelectric Point: 4.4610

>NTDB_id=768807 O8413_RS09995 WP_041942471.1 2153778..2155502(-) (pilQ) [Vibrio furnissii strain MT14]
MIKGLNASTAWGIRCLFVGVCLLLSAATFAQEAAANQLKNIDFRINKDKAAVLIVELASASAVVDVQKTEQGLTIELLKT
DVSDDKLYLVDVRDFATAVENIEVFRQQPSTVLVATVQGAFNYEYSLKGNYLEVVISKVSAQEPQPKSVLEKEGKLISIN
FQDIPVRNVLQLIADYNQFNLVVSDSVSGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDRREQQQLEK
ARMAEELGDLQSEIIKINFAKASDIAQMIGGDGAVNMLSERGSISIDERTNALLLRELPDNIHVIRDIVASLDIPVKQVQ
IEARIVTVNEGDLDELGVRWGFSSTNGSTTTGGSIESNLGAIGLYDGSSDSGDGAAIDDFLNVNLAATSANASSIAFQVA
KLGADTLLDLELSAMQRESKAEIISSPRLITTNKQPAYIEQGTEIPYLESSSSGATSVAFKKAVLSLKVTPQITPDNRLV
LDLSVTQDRPGEVVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVTSSVDKVPLLGDLPLLGALFRRSYEQMGK
SELLIFVTPKVVIQ

Nucleotide


Download         Length: 1725 bp        

>NTDB_id=768807 O8413_RS09995 WP_041942471.1 2153778..2155502(-) (pilQ) [Vibrio furnissii strain MT14]
ATGATTAAAGGACTAAATGCCTCCACGGCTTGGGGAATACGTTGTCTGTTTGTGGGCGTATGCCTGCTGTTATCTGCCGC
CACATTTGCTCAGGAAGCGGCGGCCAATCAGCTGAAAAATATCGACTTTCGCATCAATAAAGACAAAGCGGCGGTGTTGA
TTGTCGAACTGGCGTCGGCATCTGCGGTGGTGGATGTGCAGAAAACGGAGCAAGGATTGACGATTGAATTGCTTAAAACC
GATGTTAGCGATGACAAACTGTATTTGGTGGACGTGCGCGATTTTGCCACGGCGGTGGAAAACATCGAAGTGTTTCGCCA
GCAACCGAGCACGGTGCTGGTGGCGACGGTGCAAGGGGCATTCAATTACGAATACAGCCTGAAAGGCAATTACTTGGAGG
TGGTGATCAGCAAAGTCAGTGCGCAAGAACCGCAACCGAAAAGTGTCTTAGAAAAAGAAGGCAAGCTGATTTCGATTAAC
TTCCAAGACATTCCCGTGCGCAACGTGCTGCAGCTCATCGCCGACTACAACCAGTTCAACTTGGTGGTCTCTGATTCCGT
CAGCGGTAACTTGACATTGCGCCTCGATGGCGTGCCGTGGCAACAAGTGCTGGATATCATTTTACAAGTCAAAGGGCTGG
ACAAACGGGTCGATGGCAACGTCATTCTGGTGGCACCCAAAGAGGAGCTGGACCGACGCGAGCAGCAGCAATTGGAAAAG
GCGCGCATGGCGGAAGAGCTGGGCGATCTGCAATCGGAAATCATCAAAATCAATTTCGCCAAGGCATCTGACATTGCACA
GATGATTGGCGGCGATGGTGCGGTGAACATGTTGTCGGAGCGTGGCTCCATCAGTATTGATGAGCGCACCAACGCCTTGC
TACTGCGCGAGCTGCCTGACAACATTCACGTGATCCGCGACATCGTGGCCTCTCTCGACATTCCGGTCAAACAAGTGCAA
ATCGAAGCGCGCATCGTCACGGTGAATGAGGGCGATTTGGATGAGCTTGGCGTGCGCTGGGGCTTTAGCTCCACCAATGG
GAGCACCACGACCGGGGGTTCGATTGAAAGTAATCTCGGTGCCATTGGGTTGTATGACGGCAGCAGTGATTCGGGCGATG
GCGCCGCGATTGATGATTTCCTCAATGTGAACCTCGCCGCCACCTCCGCCAATGCATCCAGTATTGCGTTTCAAGTGGCC
AAGCTGGGCGCTGACACCTTACTTGATCTTGAATTGTCGGCGATGCAGCGCGAGTCGAAAGCGGAAATTATTTCCAGCCC
AAGGCTGATCACCACCAACAAACAGCCAGCTTACATTGAACAGGGGACGGAAATTCCTTATCTCGAATCGTCCTCCAGTG
GCGCAACCAGCGTGGCATTTAAAAAAGCGGTCCTCAGCCTCAAAGTGACCCCGCAAATTACGCCTGACAACCGTTTGGTG
CTCGATTTGAGCGTGACCCAAGACCGACCGGGTGAAGTGGTGAAAACGGGCACGGGCGAAGCGGTGGCGATTAACACGCA
GCGTATTGGGACCCAAGTGTTAGTTAATAACGGTGAAACCGTGGTGCTGGGCGGCATTTTTCAACACAGCGTGACCAGTT
CTGTGGACAAAGTGCCCTTGCTTGGGGACTTGCCATTACTGGGCGCGCTGTTCCGTCGCAGCTACGAACAGATGGGGAAA
AGTGAGCTACTGATCTTCGTCACGCCGAAAGTTGTGATTCAGTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(253-318)

Secretin and TonB N terminus short domain (STN)

(179-227)

Bacterial type II and III secretion system protein (Secretin)

(414-572)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

80.729

100

0.81
  pilQ Vibrio cholerae strain A1552

80.729

100

0.81
  pilQ Vibrio campbellii strain DS40M4

77.413

95.645

0.74
  pilQ Pseudomonas aeruginosa PAK

39.86

99.652

0.397
  pilQ Legionella pneumophila strain ERS1305867

41.575

95.122

0.395
  pilQ Legionella pneumophila strain Lp02

41.575

95.122

0.395
  comQ Acinetobacter baylyi ADP1

38.737

100

0.395
  pilQ Acinetobacter baumannii D1279779

37.113

100

0.376
  pilQ Acinetobacter baumannii strain A118

37.113

100

0.376