Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O8I58_RS15005 Genome accession   NZ_CP114978
Coordinates   3429521..3430654 (-) Length   377 a.a.
NCBI ID   WP_298317743.1    Uniprot ID   -
Organism   MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3424521..3435654
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I58_RS14970 (O8I58_14970) coq7 3424718..3425362 (-) 645 WP_298317726.1 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinone monooxygenase -
  O8I58_RS14975 (O8I58_14975) rplM 3425606..3426034 (+) 429 WP_298317729.1 50S ribosomal protein L13 -
  O8I58_RS14980 (O8I58_14980) rpsI 3426037..3426429 (+) 393 WP_093133728.1 30S ribosomal protein S9 -
  O8I58_RS14990 (O8I58_14990) - 3426895..3427578 (+) 684 WP_298317736.1 2OG-Fe dioxygenase family protein -
  O8I58_RS14995 (O8I58_14995) - 3427648..3428637 (+) 990 WP_298317739.1 fumarylacetoacetate hydrolase family protein -
  O8I58_RS15000 (O8I58_15000) maiA 3428714..3429391 (+) 678 WP_298317742.1 maleylacetoacetate isomerase -
  O8I58_RS15005 (O8I58_15005) pilU 3429521..3430654 (-) 1134 WP_298317743.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O8I58_RS15010 (O8I58_15010) - 3430753..3431142 (+) 390 WP_298317746.1 DUF4398 domain-containing protein -
  O8I58_RS15015 (O8I58_15015) - 3431139..3431801 (+) 663 WP_298317749.1 hypothetical protein -
  O8I58_RS15020 (O8I58_15020) - 3431761..3432021 (+) 261 WP_298317752.1 hypothetical protein -
  O8I58_RS15025 (O8I58_15025) - 3432356..3432574 (+) 219 WP_298317754.1 YdcH family protein -
  O8I58_RS15030 (O8I58_15030) - 3432771..3434147 (+) 1377 WP_298317757.1 pyridoxal-phosphate dependent enzyme -
  O8I58_RS15035 (O8I58_15035) - 3434237..3435424 (+) 1188 WP_298317760.1 cystathionine gamma-synthase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41724.99 Da        Isoelectric Point: 6.0148

>NTDB_id=767672 O8I58_RS15005 WP_298317743.1 3429521..3430654(-) (pilU) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNIAVALADAGR
FRVNVFKQRGEVGMVIRAIRSRIPSIEELQLPQVLKDIIMTPRGLVLLVGSTGSGKSTSLASMIDFRNSSTTGHILTIED
PIEYLHKHKQSIVNQREVGLDTHAFQNALKNAMREAPDVILIGEILDATTMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPETAHKNVLMNLALNLRAVVSQRLVKGVDGRRIPAAEVLINTPVIRDLLRRGQIHEIKQAMEESLEDGMESFDQCL
FRMVKEGRIAQEEALRAADSRDGLALKFRLSEGSSGEHDPYADMYENASPNITHGFG

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=767672 O8I58_RS15005 WP_298317743.1 3429521..3430654(-) (pilU) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
ATGGACATCGGCTATTTCCTCAAGCTGATGACGGAAAAGAACGCGTCGGACATGTTCCTGACCACCGGCGCGCCCGTCTA
TATCAAGATCGAAGGCAAGCTCTACCCGCTGGGTAACACGGGCCTGCCGCCCGGCATGGTCAAGAAGATCGCCTATTCGC
TGATGGACGAAGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAGCTCAACATCGCCGTGGCCCTGGCCGACGCCGGGCGT
TTCCGCGTGAATGTGTTCAAGCAGCGCGGCGAAGTGGGCATGGTCATCCGCGCCATCCGCAGCCGGATCCCCAGCATCGA
GGAACTGCAGCTGCCGCAGGTACTGAAGGACATCATCATGACCCCGCGCGGGCTGGTGCTGCTGGTCGGCTCGACCGGCT
CGGGCAAGTCCACCTCGCTGGCCTCGATGATCGATTTCCGCAACTCCAGCACCACCGGCCACATCCTCACCATCGAGGAT
CCGATCGAGTACCTGCACAAGCACAAGCAGTCGATCGTGAACCAGCGTGAGGTCGGCCTGGACACCCACGCCTTCCAAAA
CGCGCTGAAGAACGCCATGCGCGAGGCACCGGACGTGATCCTGATCGGCGAGATCCTGGATGCCACCACGATGGAGGCCG
CGATCGCCTTCGCCGAAACCGGCCACCTGTGCCTGGCCACGCTGCACTCCAACAACGCCGACCAGACCATCGAGCGCATC
CTCAACTTCTTCCCGGAAACCGCGCACAAGAACGTGCTGATGAACCTGGCGCTGAACCTGCGCGCGGTGGTGTCCCAGCG
CCTGGTCAAGGGCGTGGACGGCCGCCGCATCCCGGCAGCCGAAGTGCTGATCAACACCCCCGTGATCCGCGACCTGCTGC
GCCGCGGCCAGATCCACGAGATCAAGCAGGCCATGGAAGAGTCGCTCGAAGACGGCATGGAATCCTTCGACCAGTGCCTG
TTCCGCATGGTCAAGGAAGGCAGGATCGCGCAGGAGGAAGCGCTGCGCGCAGCCGATTCACGCGACGGCCTGGCGCTGAA
GTTCCGCCTGTCAGAAGGCTCATCGGGCGAACACGACCCATACGCGGACATGTACGAAAACGCCTCGCCCAACATCACCC
ACGGCTTCGGCTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(80-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

50.696

95.225

0.483
  pilU Acinetobacter baylyi ADP1

47.554

97.613

0.464
  pilU Vibrio cholerae strain A1552

48.023

93.899

0.451
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.536

100

0.438
  pilT Legionella pneumophila strain ERS1305867

41.124

89.655

0.369
  pilT Legionella pneumophila strain Lp02

41.124

89.655

0.369
  pilT Pseudomonas aeruginosa PAK

40.828

89.655

0.366
  pilT Acinetobacter baumannii strain A118

40.533

89.655

0.363
  pilT Acinetobacter nosocomialis M2

40.533

89.655

0.363
  pilT Acinetobacter baumannii D1279779

40.533

89.655

0.363
  pilT Acinetobacter baylyi ADP1

40

90.186

0.361