Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   O8I58_RS07110 Genome accession   NZ_CP114978
Coordinates   1629422..1630552 (-) Length   376 a.a.
NCBI ID   WP_298321778.1    Uniprot ID   -
Organism   MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1624422..1635552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I58_RS07090 (O8I58_07090) - 1624904..1625449 (+) 546 WP_298321773.1 Dps family protein -
  O8I58_RS07095 (O8I58_07095) recQ 1625702..1627498 (+) 1797 WP_298321774.1 DNA helicase RecQ -
  O8I58_RS07100 (O8I58_07100) - 1627548..1628117 (+) 570 WP_298321776.1 DNA-3-methyladenine glycosylase I -
  O8I58_RS07105 (O8I58_07105) - 1628502..1629197 (-) 696 WP_298322821.1 YitT family protein -
  O8I58_RS07110 (O8I58_07110) pilU 1629422..1630552 (-) 1131 WP_298321778.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O8I58_RS07115 (O8I58_07115) pilT 1630677..1631714 (-) 1038 WP_298321780.1 type IV pilus twitching motility protein PilT Machinery gene
  O8I58_RS07120 (O8I58_07120) - 1631844..1632497 (+) 654 WP_298321782.1 YggS family pyridoxal phosphate-dependent enzyme -
  O8I58_RS07125 (O8I58_07125) proC 1632566..1633417 (+) 852 WP_298321784.1 pyrroline-5-carboxylate reductase -
  O8I58_RS07130 (O8I58_07130) - 1633421..1633909 (+) 489 WP_298321786.1 DUF4426 domain-containing protein -
  O8I58_RS07135 (O8I58_07135) soxR 1633995..1634429 (-) 435 WP_298321788.1 redox-sensitive transcriptional activator SoxR -

Sequence

Protein


Download         Length: 376 a.a.        Molecular weight: 41930.29 Da        Isoelectric Point: 6.8286

>NTDB_id=767668 O8I58_RS07110 WP_298321778.1 1629422..1630552(-) (pilU) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
MSTIDFTSFLKLMAHQKASDLFITSGMPPSIKVHGKLSPITQTPLTPQQSRDLVLNVMTPAQREEFEKTHECNFAIGVAG
VGRFRVSCFYQRNQVGMVLRRIETKIPSVDELNLPPIIKTLAMTKRGIILFVGATGTGKSTSLAAMIGYRNLNSTGHIIT
IEDPIEFVHKHEGCIITQREVGIDTDSWENALKNTLRQAPDVIMIGEVRTREGMDHAIAFAETGHLVLCTLHANNANQAM
DRIINFFPEDRRNQLLMDLSLNLRGVVAQQLVPTPDGKARRVAMEIMLGTPLIQDYIRDGEIHKLKEVMKESTNLGMKTF
DQSLFELYQAGEISYEDALRYADSQNEVRLRIKLAQGGDARTLAQGLDGVEVAEVR

Nucleotide


Download         Length: 1131 bp        

>NTDB_id=767668 O8I58_RS07110 WP_298321778.1 1629422..1630552(-) (pilU) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
ATGAGCACCATCGACTTCACCTCGTTCCTCAAGCTGATGGCGCACCAGAAGGCGTCGGACCTGTTCATCACCTCGGGCAT
GCCGCCGTCGATCAAGGTGCACGGCAAGCTCTCGCCGATCACCCAGACGCCGCTGACGCCGCAGCAGTCGCGCGACCTGG
TGCTGAACGTGATGACCCCAGCCCAGCGCGAGGAATTCGAAAAGACCCACGAGTGCAACTTCGCCATCGGCGTGGCCGGC
GTGGGGCGCTTCCGCGTCAGCTGCTTCTACCAGCGCAACCAGGTGGGCATGGTGCTGCGCCGGATCGAGACCAAGATCCC
CAGTGTCGATGAGCTGAACCTGCCGCCGATCATCAAGACCCTGGCGATGACCAAGCGCGGCATCATTCTTTTCGTCGGCG
CCACCGGCACCGGCAAGTCGACTTCGCTGGCGGCGATGATCGGCTACCGCAACCTCAATTCGACCGGCCACATCATCACC
ATCGAGGACCCGATCGAGTTCGTGCACAAGCACGAGGGCTGCATCATCACCCAGCGCGAGGTCGGCATCGACACCGACAG
CTGGGAGAACGCGCTGAAGAACACCCTGCGCCAGGCGCCGGACGTGATCATGATCGGCGAGGTGCGCACCCGCGAAGGCA
TGGACCATGCGATCGCCTTCGCCGAAACCGGCCACCTGGTGCTATGCACGCTGCACGCCAACAACGCCAACCAGGCGATG
GACCGCATCATCAACTTCTTCCCCGAAGACCGCCGCAACCAGTTGTTGATGGACCTGTCGCTGAACCTCAGGGGCGTGGT
CGCCCAGCAGCTGGTGCCTACGCCCGATGGCAAGGCCCGCCGCGTGGCGATGGAGATCATGCTGGGCACGCCGCTGATCC
AGGACTACATCCGCGACGGCGAGATCCACAAGCTCAAGGAGGTCATGAAGGAGTCGACCAACCTGGGCATGAAGACCTTC
GACCAGAGCCTGTTCGAGCTGTACCAGGCTGGCGAGATTTCCTACGAGGATGCGCTGCGTTACGCCGACTCGCAGAACGA
GGTGCGCCTGCGCATCAAGCTGGCCCAGGGCGGCGATGCCCGCACCCTGGCCCAGGGCCTGGACGGCGTCGAGGTGGCGG
AAGTCCGCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(118-273)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

66.757

97.606

0.652
  pilU Acinetobacter baylyi ADP1

63.585

94.947

0.604
  pilU Vibrio cholerae strain A1552

53.09

94.681

0.503
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.714

93.085

0.388
  pilT Acinetobacter baumannii strain A118

40.698

91.489

0.372
  pilT Acinetobacter nosocomialis M2

40.698

91.489

0.372
  pilT Acinetobacter baumannii D1279779

40.698

91.489

0.372
  pilT Legionella pneumophila strain Lp02

41.194

89.096

0.367
  pilT Legionella pneumophila strain ERS1305867

41.194

89.096

0.367
  pilT Pseudomonas aeruginosa PAK

40.597

89.096

0.362