Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   O8I58_RS07115 Genome accession   NZ_CP114978
Coordinates   1630677..1631714 (-) Length   345 a.a.
NCBI ID   WP_298321780.1    Uniprot ID   -
Organism   MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1625677..1636714
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O8I58_RS07095 (O8I58_07095) recQ 1625702..1627498 (+) 1797 WP_298321774.1 DNA helicase RecQ -
  O8I58_RS07100 (O8I58_07100) - 1627548..1628117 (+) 570 WP_298321776.1 DNA-3-methyladenine glycosylase I -
  O8I58_RS07105 (O8I58_07105) - 1628502..1629197 (-) 696 WP_298322821.1 YitT family protein -
  O8I58_RS07110 (O8I58_07110) pilU 1629422..1630552 (-) 1131 WP_298321778.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  O8I58_RS07115 (O8I58_07115) pilT 1630677..1631714 (-) 1038 WP_298321780.1 type IV pilus twitching motility protein PilT Machinery gene
  O8I58_RS07120 (O8I58_07120) - 1631844..1632497 (+) 654 WP_298321782.1 YggS family pyridoxal phosphate-dependent enzyme -
  O8I58_RS07125 (O8I58_07125) proC 1632566..1633417 (+) 852 WP_298321784.1 pyrroline-5-carboxylate reductase -
  O8I58_RS07130 (O8I58_07130) - 1633421..1633909 (+) 489 WP_298321786.1 DUF4426 domain-containing protein -
  O8I58_RS07135 (O8I58_07135) soxR 1633995..1634429 (-) 435 WP_298321788.1 redox-sensitive transcriptional activator SoxR -
  O8I58_RS07140 (O8I58_07140) - 1634527..1635915 (+) 1389 WP_298321790.1 MFS transporter -
  O8I58_RS07145 (O8I58_07145) - 1635912..1636706 (-) 795 WP_298321794.1 ABC transporter substrate-binding protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38727.45 Da        Isoelectric Point: 6.6655

>NTDB_id=767669 O8I58_RS07115 WP_298321780.1 1630677..1631714(-) (pilT) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEELGCPPLFREMIQQPQGLILVTGPTGSGKSTTLAAMIDFINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDYILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRSVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSAIQTGQQYGMMTLDQHL
QDLVKRSQITRNQAKEYAKDKRLFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=767669 O8I58_RS07115 WP_298321780.1 1630677..1631714(-) (pilT) [MAG: Pseudoxanthomonas sp. isolate Chal_Metagen_Bin2]
ATGGATATCGCCGAACTACTGGCCTTCTCGGTCAAGAACAAGGCTTCGGACCTGCATCTGTCGGCCGGGCTGCCGCCGAT
GATCCGCGTGGACGGCGACGTGCGCCGCATCAACATTCCCGCGCTGGACCACAAGCAGGTGCACGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCCTTCGAGATTCCCTCGCTGGCCCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGCACCATTCCGTCCGAAGTGCTGACCCTGGA
AGAGCTGGGTTGCCCGCCGCTGTTCCGCGAAATGATCCAGCAGCCGCAGGGCTTGATCCTGGTCACCGGCCCGACCGGCT
CGGGCAAGTCGACCACGCTGGCGGCGATGATCGACTTCATCAACAAGAACGAATACGGCCACATCCTCACCGTCGAGGAC
CCGATCGAATTCGTCCACACCTCGCAGAAGTGCCTGATCAACCAGCGCGAAGTGCACCGCGACACGCACGGCTTCAACGA
GGCATTGCGTTCGGCCCTGCGCGAAGACCCTGACTACATCCTGGTCGGCGAGTTACGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCGGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCCGCAGGCGAAAAGCCGATGGTCCGCTCGATGCTATCCGAGTCGCTGCGTTCGGTGATCTCGCAGGC
GCTGCTGAAGAAGGTGGGGGGCGGACGCACCGCAGCCTGGGAAATCATGGTCGGCACGCCGGCCATCCGTAACCTGATCC
GCGAGGACAAGGTCGCGCAGATGTACTCGGCCATCCAGACCGGCCAGCAGTACGGCATGATGACCCTGGACCAACATCTG
CAGGACCTGGTCAAGCGCAGCCAGATCACGCGCAACCAGGCCAAGGAATACGCCAAGGACAAGCGTCTGTTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

78.488

99.71

0.783
  pilT Acinetobacter baumannii D1279779

78.488

99.71

0.783
  pilT Acinetobacter baumannii strain A118

78.488

99.71

0.783
  pilT Pseudomonas stutzeri DSM 10701

77.616

99.71

0.774
  pilT Acinetobacter baylyi ADP1

77.326

99.71

0.771
  pilT Pseudomonas aeruginosa PAK

78.466

98.261

0.771
  pilT Legionella pneumophila strain Lp02

74.419

99.71

0.742
  pilT Legionella pneumophila strain ERS1305867

74.419

99.71

0.742
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

70.213

95.362

0.67
  pilT Vibrio cholerae strain A1552

70.213

95.362

0.67
  pilT Neisseria meningitidis 8013

64.809

98.841

0.641
  pilT Neisseria gonorrhoeae MS11

64.516

98.841

0.638
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.565

100

0.496
  pilU Vibrio cholerae strain A1552

39.943

100

0.403
  pilU Pseudomonas stutzeri DSM 10701

39.528

98.261

0.388
  pilU Acinetobacter baylyi ADP1

39.118

98.551

0.386