Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OSW16_RS01920 Genome accession   NZ_CP113097
Coordinates   395820..397088 (+) Length   422 a.a.
NCBI ID   WP_267820355.1    Uniprot ID   -
Organism   Pseudomonas putida strain WYTS.T61     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 390820..402088
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSW16_RS01900 (OSW16_01900) - 390867..393320 (-) 2454 WP_267820349.1 penicillin-binding protein 1A -
  OSW16_RS01905 (OSW16_01905) pilM 393499..394347 (+) 849 WP_267820351.1 type IV pilus biogenesis protein PilM -
  OSW16_RS01910 (OSW16_01910) - 394347..394883 (+) 537 WP_241805105.1 PilN domain-containing protein -
  OSW16_RS01915 (OSW16_01915) - 394880..395896 (+) 1017 WP_267820353.1 pilus assembly protein PilP -
  OSW16_RS01920 (OSW16_01920) pilQ 395820..397088 (+) 1269 WP_267820355.1 type IV pilus secretin PilQ Machinery gene
  OSW16_RS01925 (OSW16_01925) aroK 397093..397611 (+) 519 WP_241805111.1 shikimate kinase AroK -
  OSW16_RS01930 (OSW16_01930) aroB 397706..398803 (+) 1098 WP_267820357.1 3-dehydroquinate synthase -
  OSW16_RS01935 (OSW16_01935) - 398819..400411 (+) 1593 WP_267820359.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45687.52 Da        Isoelectric Point: 4.7358

>NTDB_id=760169 OSW16_RS01920 WP_267820355.1 395820..397088(+) (pilQ) [Pseudomonas putida strain WYTS.T61]
MNGMMKWLVAALLPMPSLAATPYQGEPVSLNFQDVEVRLVLQVLADYAGINLVASDTVQGSITLRLQDVPWDQALELVLR
SKGLARREEGKVLVVAPAAELAEQALGARMGLELEAQSQPLRRELLPIQHAAAPELAELLLATLADDGILTGRGSLHVDV
RTNTLVAHQPEGRLAELRQLVAQLDVPVRQVEIEARIVEANVDYEHSLGVRWGPRLYGEAPALGADVFVDLGVERAGSSI
GLGVLRGGVLLDLELSAMEKTGNGEIISQPKVVTANNETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVRVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDVLQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=760169 OSW16_RS01920 WP_267820355.1 395820..397088(+) (pilQ) [Pseudomonas putida strain WYTS.T61]
ATGAACGGGATGATGAAGTGGCTGGTGGCGGCCTTGCTGCCGATGCCTTCGCTGGCAGCGACGCCTTATCAGGGTGAACC
GGTTTCGCTGAACTTCCAGGACGTGGAAGTGCGCCTTGTGTTGCAGGTGCTGGCCGATTATGCCGGCATCAACCTGGTCG
CCAGCGACACGGTGCAGGGCAGTATCACCTTGCGTCTGCAGGATGTTCCGTGGGATCAGGCGCTGGAATTGGTGCTGCGC
AGCAAGGGGCTGGCCCGGCGGGAAGAAGGCAAGGTACTGGTGGTGGCGCCAGCGGCTGAGCTGGCCGAACAGGCGCTGGG
GGCGCGTATGGGGTTGGAGTTGGAGGCCCAGTCGCAGCCGTTGCGCCGGGAGTTGCTGCCGATACAACACGCCGCGGCCC
CAGAACTTGCCGAGTTGCTGCTGGCTACGTTGGCCGATGACGGCATTCTGACTGGGCGCGGTAGTTTGCATGTGGATGTG
CGCACCAATACGCTGGTCGCTCACCAGCCTGAGGGCCGGCTTGCCGAGTTGCGCCAGTTGGTTGCGCAGCTGGATGTGCC
TGTACGGCAGGTCGAGATCGAGGCCCGCATCGTGGAGGCCAATGTCGATTACGAGCACAGCCTTGGGGTGCGCTGGGGCC
CGCGTCTCTATGGCGAAGCCCCTGCGTTGGGCGCGGACGTATTTGTCGATCTTGGGGTGGAGCGGGCCGGTTCCAGCATC
GGCTTGGGCGTATTGCGCGGGGGCGTGCTGCTGGACCTTGAACTCAGCGCCATGGAGAAGACCGGTAATGGCGAAATCAT
CTCCCAGCCCAAGGTGGTAACGGCCAATAACGAGACTGCCCGTATCCTCAAAGGCACAGAAGTCCCTTACCAGGAGACCT
CTAAGAGTGGCGCTACGTCGGTTGCATTCCGCGAGGCGTCGCTGTCGCTGGAGGTCACGCCGCAGATTACGCCGGACGGC
AAGGTGATCATGACGGTCAAGGTGACCAAGGACGAGCCTGATTTCGTCAATGCCTTGAACGATGTGCCGCCGATCCGCAA
GAACGAGGTCAATGCCAAGGTCCGGGTAGCGGATGGGGAAACCATCGTCATTGGCGGTGTGTACTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGTATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.277

100

0.609
  pilQ Acinetobacter baumannii D1279779

40

100

0.441
  pilQ Acinetobacter baumannii strain A118

40

100

0.441
  comQ Acinetobacter baylyi ADP1

41.124

100

0.434
  pilQ Legionella pneumophila strain Lp02

41.514

100

0.429
  pilQ Legionella pneumophila strain ERS1305867

41.514

100

0.429
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

40.952

99.526

0.408
  pilQ Vibrio cholerae strain A1552

40.952

99.526

0.408
  pilQ Vibrio campbellii strain DS40M4

39.12

100

0.4
  pilQ Neisseria gonorrhoeae strain FA1090

36.493

100

0.365
  pilQ Neisseria meningitidis MC58

36.019

100

0.36
  pilQ Neisseria meningitidis M1080

36.019

100

0.36