Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   KI243_RS27865 Genome accession   NZ_AP021904
Coordinates   6311980..6313197 (+) Length   405 a.a.
NCBI ID   WP_177127818.1    Uniprot ID   -
Organism   Pseudomonas sp. Ost2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6306980..6318197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS27850 (PHLH7_54970) - 6307886..6309532 (-) 1647 WP_178989962.1 hypothetical protein -
  KI243_RS27855 (PHLH7_54980) pilA2 6309650..6310051 (-) 402 WP_177127819.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  KI243_RS27860 (PHLH7_54990) pilB 6310276..6311976 (+) 1701 WP_177127856.1 type IV-A pilus assembly ATPase PilB Machinery gene
  KI243_RS27865 (PHLH7_55000) pilC 6311980..6313197 (+) 1218 WP_177127818.1 type II secretion system F family protein Machinery gene
  KI243_RS27870 (PHLH7_55010) pilD 6313200..6314072 (+) 873 WP_178989964.1 A24 family peptidase Machinery gene
  KI243_RS27875 (PHLH7_55020) coaE 6314069..6314692 (+) 624 WP_177084074.1 dephospho-CoA kinase -
  KI243_RS27880 (PHLH7_55030) yacG 6314689..6314892 (+) 204 WP_017123507.1 DNA gyrase inhibitor YacG -
  KI243_RS27885 (PHLH7_55040) - 6314898..6315113 (-) 216 WP_178989966.1 hypothetical protein -
  KI243_RS27890 (PHLH7_55050) - 6315192..6315890 (-) 699 WP_017123505.1 energy-coupling factor ABC transporter permease -
  KI243_RS27895 (PHLH7_55060) - 6316026..6316655 (+) 630 WP_042934238.1 DUF1780 domain-containing protein -
  KI243_RS27900 (PHLH7_55070) - 6316723..6316896 (+) 174 WP_017123503.1 DUF3094 family protein -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44289.29 Da        Isoelectric Point: 10.1966

>NTDB_id=75719 KI243_RS27865 WP_177127818.1 6311980..6313197(+) (pilC) [Pseudomonas sp. Ost2]
MATKAVKISVYAWEGTDKKGTKMSGELTGHNPALVKAQLRKQGINPGKVRKKSASIFSKGKRIKPMDIALFTRQMATMMK
AGVPLLQSFDIIAEGFDNPNMRKLVDDIKQEVAAGNSFATSLRKKPEYFDELYCNLVDAGEQAGALESLLDRVATYKEKT
EALKAKIKKAMNYPIAVILVAIIVSGILLIKVVPQFQAIFAGFGAQLPAFTLMVIGLSQILQDWWWIVILALVGGIYAFR
RAHKTSERFRNRLDRMLLKMPIIGPLLYKSAVARFARTLSTTFAAGVPLVEALDSVSGATGNIVFKNAVNRVKQDVSTGM
QLNFSMRSTGVFPSMALQMTAIGEESGSLDDMLDKVATYYEAEVDNMVDSLTSLMEPIIMSVLGVIVGGLVIAMYLPIFK
LGSVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=75719 KI243_RS27865 WP_177127818.1 6311980..6313197(+) (pilC) [Pseudomonas sp. Ost2]
ATGGCGACGAAAGCAGTAAAGATCAGCGTGTATGCATGGGAGGGTACCGACAAGAAAGGTACCAAGATGAGTGGCGAACT
CACGGGTCACAACCCGGCACTGGTCAAGGCACAACTGCGCAAACAGGGCATCAATCCGGGCAAGGTGCGCAAGAAAAGCG
CGTCGATCTTCAGCAAGGGCAAGCGTATCAAGCCCATGGATATCGCGCTGTTTACCCGCCAGATGGCGACCATGATGAAG
GCCGGCGTCCCCTTGCTACAGTCTTTCGACATCATTGCCGAGGGGTTCGATAACCCGAATATGCGCAAGCTGGTGGACGA
CATCAAGCAGGAGGTCGCTGCCGGCAACAGCTTTGCTACCTCTCTGCGCAAGAAACCCGAGTATTTCGATGAGTTGTATT
GCAACCTGGTGGACGCTGGCGAACAGGCTGGCGCCTTGGAAAGCCTGCTGGACCGGGTAGCCACCTATAAGGAAAAAACC
GAAGCCCTCAAAGCCAAGATCAAAAAAGCCATGAACTACCCAATTGCGGTAATACTCGTGGCAATTATCGTGTCCGGTAT
CCTGCTGATCAAGGTAGTGCCACAGTTCCAGGCGATCTTTGCTGGCTTCGGCGCCCAACTGCCTGCCTTCACTCTGATGG
TGATCGGCCTTTCGCAGATACTCCAAGACTGGTGGTGGATTGTGATCCTTGCACTGGTAGGTGGGATCTATGCGTTCAGA
CGCGCACACAAGACCTCCGAGCGCTTCCGCAACCGGCTGGACCGAATGCTCTTGAAGATGCCGATTATCGGTCCATTGCT
CTACAAATCTGCCGTAGCCCGATTTGCACGGACACTATCGACCACCTTCGCCGCCGGTGTGCCGCTGGTGGAAGCCTTGG
ACTCCGTCTCGGGTGCTACAGGTAACATCGTCTTCAAAAATGCCGTCAACCGCGTCAAACAGGATGTCTCCACAGGCATG
CAACTGAACTTCTCGATGCGATCTACCGGCGTTTTTCCCAGCATGGCGCTCCAGATGACCGCCATTGGTGAGGAGTCCGG
CTCCCTGGACGATATGCTCGACAAGGTCGCGACCTACTACGAAGCCGAAGTCGACAATATGGTCGATAGCCTGACCAGCC
TGATGGAGCCCATCATCATGAGCGTGCTCGGCGTAATCGTCGGCGGCCTGGTCATTGCCATGTACCTGCCCATCTTCAAG
CTTGGCTCAGTAGTCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

78.272

100

0.783
  pilC Acinetobacter baylyi ADP1

59.069

100

0.595
  pilC Acinetobacter baumannii D1279779

58.911

99.753

0.588
  pilC Legionella pneumophila strain ERS1305867

54.798

97.778

0.536
  pilC Vibrio cholerae strain A1552

43.382

100

0.437
  pilG Neisseria gonorrhoeae MS11

44.584

98.025

0.437
  pilG Neisseria meningitidis 44/76-A

44.584

98.025

0.437
  pilC Vibrio campbellii strain DS40M4

43.103

100

0.432
  pilC Thermus thermophilus HB27

38.155

99.012

0.378

Multiple sequence alignment