Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   F7G23_RS21105 Genome accession   NZ_AP024921
Coordinates   4160450..4160917 (+) Length   155 a.a.
NCBI ID   WP_000381746.1    Uniprot ID   Q8X892
Organism   Escherichia coli O145:H28 strain 12E115     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4155450..4165917
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  F7G23_RS21060 (EC12E115_4051) rplP 4155560..4155970 (-) 411 WP_000941212.1 50S ribosomal protein L16 -
  F7G23_RS21065 (EC12E115_4052) rpsC 4155983..4156684 (-) 702 WP_000529945.1 30S ribosomal protein S3 -
  F7G23_RS21070 (EC12E115_4053) rplV 4156702..4157034 (-) 333 WP_000447529.1 50S ribosomal protein L22 -
  F7G23_RS21075 (EC12E115_4054) rpsS 4157049..4157327 (-) 279 WP_001138117.1 30S ribosomal protein S19 -
  F7G23_RS21080 (EC12E115_4055) rplB 4157344..4158165 (-) 822 WP_000301864.1 50S ribosomal protein L2 -
  F7G23_RS21085 (EC12E115_4056) rplW 4158183..4158485 (-) 303 WP_000617544.1 50S ribosomal protein L23 -
  F7G23_RS21090 (EC12E115_4057) rplD 4158482..4159087 (-) 606 WP_000424395.1 50S ribosomal protein L4 -
  F7G23_RS21095 (EC12E115_4058) rplC 4159098..4159727 (-) 630 WP_000579833.1 50S ribosomal protein L3 -
  F7G23_RS21100 (EC12E115_4059) rpsJ 4159760..4160071 (-) 312 WP_001181004.1 30S ribosomal protein S10 -
  F7G23_RS21105 (EC12E115_4060) pilD 4160450..4160917 (+) 468 WP_000381746.1 A24 family peptidase Machinery gene
  F7G23_RS21110 (EC12E115_4061) bfr 4160914..4161390 (-) 477 WP_000675515.1 bacterioferritin -
  F7G23_RS21115 (EC12E115_4062) bfd 4161463..4161657 (-) 195 WP_000289090.1 bacterioferritin-associated ferredoxin -
  F7G23_RS21120 (EC12E115_4063) tuf 4161840..4163024 (-) 1185 WP_000031783.1 elongation factor Tu -
  F7G23_RS21125 (EC12E115_4064) fusA 4163095..4165209 (-) 2115 WP_000124700.1 elongation factor G -
  F7G23_RS21130 (EC12E115_4065) rpsG 4165306..4165776 (-) 471 WP_001138043.1 30S ribosomal protein S7 -

Sequence

Protein


Download         Length: 155 a.a.        Molecular weight: 17099.29 Da        Isoelectric Point: 8.1343

>NTDB_id=75719 F7G23_RS21105 WP_000381746.1 4160450..4160917(+) (pilD) [Escherichia coli O145:H28 strain 12E115]
MDTTLPFLILYACLSALLFFWDAKHGLLPDRFTCPLLWSGLLFYQVCHPDGLADALWGAIIGYGTFAVIYWGYRILRHKE
GLGYGDVKFLAALGAWHSWAFLPRLVFLAASFACGAVVIGLLMRGKESLKNPLPFGPFLAAAGFVIGWDSLLIGR

Nucleotide


Download         Length: 468 bp        

>NTDB_id=75719 F7G23_RS21105 WP_000381746.1 4160450..4160917(+) (pilD) [Escherichia coli O145:H28 strain 12E115]
ATGGACACTACCCTTCCCTTTCTAATTTTGTACGCTTGTTTATCAGCCCTACTTTTTTTCTGGGATGCAAAGCATGGCTT
GTTACCTGACAGATTTACGTGCCCGTTACTCTGGTCAGGTCTTTTGTTCTATCAAGTTTGTCATCCTGATGGTTTAGCCG
ATGCGTTATGGGGGGCAATTATTGGTTACGGCACATTCGCTGTCATTTACTGGGGGTATCGCATACTGCGCCATAAAGAA
GGATTAGGCTATGGTGATGTGAAGTTTCTCGCCGCCCTTGGCGCCTGGCATTCATGGGCTTTCTTACCACGACTGGTTTT
CCTTGCTGCCTCATTCGCTTGCGGAGCCGTAGTTATTGGTCTGCTCATGAGAGGAAAAGAATCATTAAAAAACCCGCTGC
CTTTTGGGCCATTTCTGGCGGCTGCGGGTTTCGTTATAGGCTGGGATAGTTTGCTGATAGGCCGTTAA

Domains

Predicted by InterProScan.

Type IV leader peptidase family (Peptidase_A24)

(11-117)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q8X892

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

43.478

89.032

0.387

Multiple sequence alignment