Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   KI243_RS27855 Genome accession   NZ_AP021904
Coordinates   6309650..6310051 (-) Length   133 a.a.
NCBI ID   WP_177127819.1    Uniprot ID   -
Organism   Pseudomonas sp. Ost2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 6289356..6309532 6309650..6310051 flank 118


Gene organization within MGE regions


Location: 6289356..6310051
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS27770 (PHLH7_54840) - 6289356..6291848 (-) 2493 WP_212810127.1 toll/interleukin-1 receptor domain-containing protein -
  KI243_RS27775 - 6292148..6292435 (-) 288 WP_212811253.1 helix-turn-helix transcriptional regulator -
  KI243_RS27780 - 6292547..6292873 (-) 327 WP_212810128.1 type II toxin-antitoxin system RelE/ParE family toxin -
  KI243_RS27785 (PHLH7_54850) - 6293201..6293890 (+) 690 WP_212810129.1 hypothetical protein -
  KI243_RS27790 (PHLH7_54860) - 6293891..6294844 (+) 954 WP_212810130.1 tyrosine-type recombinase/integrase -
  KI243_RS27795 (PHLH7_54870) - 6295192..6295869 (+) 678 WP_212810131.1 hypothetical protein -
  KI243_RS27800 (PHLH7_54880) - 6296595..6297113 (-) 519 WP_212810132.1 hypothetical protein -
  KI243_RS27805 (PHLH7_54890) - 6298048..6299904 (-) 1857 WP_212810133.1 hypothetical protein -
  KI243_RS27810 (PHLH7_54900) - 6300856..6301098 (+) 243 WP_008091975.1 hypothetical protein -
  KI243_RS27815 (PHLH7_54910) - 6301091..6302017 (+) 927 WP_043273159.1 site-specific integrase -
  KI243_RS27820 (PHLH7_54930) trfA 6302958..6303863 (-) 906 WP_212810134.1 plasmid replication initiator TrfA -
  KI243_RS27825 - 6303866..6304069 (-) 204 WP_081502512.1 AlpA family transcriptional regulator -
  KI243_RS27830 (PHLH7_54940) - 6304187..6304933 (-) 747 WP_212810135.1 hypothetical protein -
  KI243_RS27835 (PHLH7_54950) - 6304930..6306147 (-) 1218 WP_212810136.1 tyrosine-type recombinase/integrase -
  KI243_RS27845 (PHLH7_54960) - 6306428..6307846 (-) 1419 WP_244861781.1 O-antigen ligase family protein -
  KI243_RS27850 (PHLH7_54970) - 6307886..6309532 (-) 1647 WP_178989962.1 hypothetical protein -
  KI243_RS27855 (PHLH7_54980) pilA2 6309650..6310051 (-) 402 WP_177127819.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13454.47 Da        Isoelectric Point: 7.5882

>NTDB_id=75717 KI243_RS27855 WP_177127819.1 6309650..6310051(-) (pilA2) [Pseudomonas sp. Ost2]
MNAQKGFTLIELMIVVAIIGILAAIALPAYNTYTQKARFAEVVSIADGYKTAVAICMSENNLSATNCSAGSNGVPTVTAA
TSSLASMSVVNGFISMTSTAAAGNTTYTLNPSVTGNSITWAVGGGCVALGFCR

Nucleotide


Download         Length: 402 bp        

>NTDB_id=75717 KI243_RS27855 WP_177127819.1 6309650..6310051(-) (pilA2) [Pseudomonas sp. Ost2]
ATGAATGCTCAGAAGGGTTTCACCTTGATCGAACTGATGATCGTTGTGGCGATCATCGGTATCTTGGCGGCGATTGCATT
GCCGGCCTATAACACCTATACGCAAAAGGCCCGCTTTGCCGAGGTCGTTTCGATCGCGGATGGATACAAGACGGCTGTGG
CAATTTGCATGTCAGAAAACAATCTGAGCGCCACTAACTGTAGTGCCGGCTCAAATGGTGTGCCAACGGTAACGGCGGCG
ACGAGCAGCCTGGCTTCCATGTCGGTTGTGAACGGTTTTATCTCAATGACTTCTACAGCTGCGGCAGGGAACACCACCTA
CACGCTTAATCCTAGTGTCACCGGTAATTCGATTACTTGGGCTGTTGGCGGGGGCTGTGTAGCTCTTGGTTTTTGCAGAT
AA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)

Pilin (bacterial filament) (Pilin)

(36-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

46.715

100

0.481
  pilA2 Legionella pneumophila strain ERS1305867

45.985

100

0.474
  comP Acinetobacter baylyi ADP1

45.185

100

0.459
  pilA Ralstonia pseudosolanacearum GMI1000

48.718

87.97

0.429
  pilA Acinetobacter baumannii strain A118

47.059

89.474

0.421
  pilA Vibrio campbellii strain DS40M4

39.85

100

0.399
  pilA Glaesserella parasuis strain SC1401

42.975

90.977

0.391
  pilA Haemophilus influenzae 86-028NP

42.149

90.977

0.383
  pilA Haemophilus influenzae Rd KW20

39.669

90.977

0.361

Multiple sequence alignment