Detailed information NTG0075718

Bioinformatically predicted

Overview

• Name: pilB
• Type: Machinery gene
• Locus tag: KI243_RS27860
• Genome accession: NZ_AP021904
• Coordinates: 6310276..6311976 (+)
• Length: 566 a.a.
• NCBI ID: WP_177127856.1
• Uniprot ID: -
• Organism: Pseudomonas sp. Ost2
• Function: power the assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	6289356..6309532	6310276..6311976	flank	744

Gene organization within MGE regions

Location: 6289356..6311976

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KI243_RS27770 (PHLH7_54840)	-	6289356..6291848 (-)	2493	WP_212810127.1	toll/interleukin-1 receptor domain-containing protein	-
KI243_RS27775	-	6292148..6292435 (-)	288	WP_212811253.1	helix-turn-helix transcriptional regulator	-
KI243_RS27780	-	6292547..6292873 (-)	327	WP_212810128.1	type II toxin-antitoxin system RelE/ParE family toxin	-
KI243_RS27785 (PHLH7_54850)	-	6293201..6293890 (+)	690	WP_212810129.1	hypothetical protein	-
KI243_RS27790 (PHLH7_54860)	-	6293891..6294844 (+)	954	WP_212810130.1	tyrosine-type recombinase/integrase	-
KI243_RS27795 (PHLH7_54870)	-	6295192..6295869 (+)	678	WP_212810131.1	hypothetical protein	-
KI243_RS27800 (PHLH7_54880)	-	6296595..6297113 (-)	519	WP_212810132.1	hypothetical protein	-
KI243_RS27805 (PHLH7_54890)	-	6298048..6299904 (-)	1857	WP_212810133.1	hypothetical protein	-
KI243_RS27810 (PHLH7_54900)	-	6300856..6301098 (+)	243	WP_008091975.1	hypothetical protein	-
KI243_RS27815 (PHLH7_54910)	-	6301091..6302017 (+)	927	WP_043273159.1	site-specific integrase	-
KI243_RS27820 (PHLH7_54930)	trfA	6302958..6303863 (-)	906	WP_212810134.1	plasmid replication initiator TrfA	-
KI243_RS27825	-	6303866..6304069 (-)	204	WP_081502512.1	AlpA family transcriptional regulator	-
KI243_RS27830 (PHLH7_54940)	-	6304187..6304933 (-)	747	WP_212810135.1	hypothetical protein	-
KI243_RS27835 (PHLH7_54950)	-	6304930..6306147 (-)	1218	WP_212810136.1	tyrosine-type recombinase/integrase	-
KI243_RS27845 (PHLH7_54960)	-	6306428..6307846 (-)	1419	WP_244861781.1	O-antigen ligase family protein	-
KI243_RS27850 (PHLH7_54970)	-	6307886..6309532 (-)	1647	WP_178989962.1	hypothetical protein	-
KI243_RS27855 (PHLH7_54980)	pilA2	6309650..6310051 (-)	402	WP_177127819.1	prepilin-type N-terminal cleavage/methylation domain-containing protein	Machinery gene
KI243_RS27860 (PHLH7_54990)	pilB	6310276..6311976 (+)	1701	WP_177127856.1	type IV-A pilus assembly ATPase PilB	Machinery gene

Sequence

Protein

• Length: 566 a.a.
• Molecular weight: 62541.65 Da
• Isoelectric Point: 6.1619

>NTDB_id=75718 KI243_RS27860 WP_177127856.1 6310276..6311976(+) (pilB) [Pseudomonas sp. Ost2]
MNDIALSGLAKQLVAAELITDKNAQQAYQQAQRNRISLVSYLVQNKLVKSRQVAEIASEHFGVALLDLSTLDKESQPKGL
VSEKLIRQHHVLPLWRRGNKLFVGISDPSNHQAITDIQFSTGLTTEAILVEDDKLADAIDKFFDSNASGLEDMADVDLEG
LDIESVDDKKHDQIAGQDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRLRTDGILREVAKPPIHLAGRIAARL
KVMASLDISERRKPQDGRIKMRISKTKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEPDQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPKQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLHNMGIPGFNIATSVNLIIAQRLARKLCTHCKREIDIPEEALLKEGFPA
DQIGKFKIYEPVGCDQCNAGYKGRVGIYEVVKNTSDLQRLIMAEGNSIEIATQMRKDGFNDLRTSGLRKAMQGLTSLEEV
NRVTKD

Nucleotide

• Length: 1701 bp

>NTDB_id=75718 KI243_RS27860 WP_177127856.1 6310276..6311976(+) (pilB) [Pseudomonas sp. Ost2]
ATGAACGATATCGCCCTTTCCGGTCTGGCCAAGCAATTGGTGGCGGCCGAGCTGATTACCGACAAGAACGCTCAGCAGGC
GTATCAGCAAGCCCAACGCAACCGCATCTCACTGGTCAGCTATCTGGTCCAGAACAAACTGGTGAAAAGCCGACAGGTTG
CGGAAATTGCCTCGGAGCATTTCGGCGTTGCCCTGCTTGACCTCAGTACCCTCGACAAGGAAAGCCAGCCCAAGGGGCTG
GTCAGTGAAAAGCTGATCCGCCAGCACCATGTCCTGCCTTTATGGCGGCGCGGCAACAAGCTCTTTGTCGGCATCTCCGA
TCCAAGCAATCATCAGGCGATCACTGATATTCAGTTCAGTACCGGCCTCACCACCGAAGCGATCCTGGTAGAAGACGACA
AGCTTGCCGATGCCATCGACAAGTTCTTCGACAGTAATGCCAGCGGCCTTGAAGACATGGCGGACGTTGACCTCGAAGGG
CTGGATATCGAATCGGTCGACGACAAGAAACATGATCAGATCGCCGGCCAGGATGCCGATGATGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTGGATGCCATTAAGGGTGGCTCCTCGGACTTGCACTTCGAACCGTATGAGAAGGTCTACCGCG
TACGCCTACGTACCGATGGTATCCTGCGGGAGGTTGCCAAGCCTCCCATCCATCTTGCCGGCCGGATCGCCGCCCGCCTG
AAGGTTATGGCCAGCCTGGATATTTCCGAGCGCCGCAAACCCCAGGACGGCCGGATCAAGATGCGTATCTCGAAGACCAA
GGCCATCGACTTCCGGGTCAATACCCTGCCGACGCTGTGGGGCGAGAAGATCGTGATGCGGATCCTTGATCCGTCCAGCG
CCCAGATGGGCATCGATGCGCTGGGCTACGAACCGGATCAGAAAGACCTCTATATGGCCGCGCTGAAGCAGCCACAAGGC
ATGATTCTGGTCACCGGCCCCACCGGTTCGGGTAAGACCGTTTCGCTGTACACCGGCTTGAATATCCTCAACACCGTGGA
TATCAATATCTCCACCGCCGAAGATCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAACCCGAAACAGG
GGCTGGACTTCGCCCAGGCATTGCGCTCGTTCCTGCGTCAGGACCCGGATGTGATCATGGTCGGTGAGATCCGCGACCTG
GAAACCGCAGAGATCGCGATCAAGGCGGCCCAGACCGGCCATATGGTGTTGTCGACCCTACACACCAACAGTGCTGCGGA
AACCCTGACGCGCCTGCATAACATGGGCATCCCCGGTTTCAACATCGCCACCTCGGTCAACCTGATCATCGCCCAGCGCC
TGGCTCGCAAACTGTGCACGCATTGCAAAAGGGAAATCGATATTCCTGAAGAAGCCTTGCTCAAGGAAGGCTTCCCGGCA
GACCAGATTGGCAAGTTCAAGATCTATGAGCCCGTCGGCTGCGACCAATGCAACGCTGGTTACAAAGGTCGCGTCGGGAT
CTATGAGGTGGTCAAGAATACGTCCGATCTGCAGCGACTGATCATGGCCGAAGGCAACTCGATCGAGATCGCCACCCAGA
TGCGCAAGGACGGCTTCAATGACCTTCGAACCTCTGGGCTGCGCAAAGCCATGCAAGGCCTGACCAGCCTGGAAGAAGTC
AACCGGGTCACCAAGGACTAA

Domains

Predicted by InterproScan.

• Type II secretion system (T2SS), protein E, N-terminal domain (MshEN) (42-147)

• Type II/IV secretion system protein (T2SSE) (190-457)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilB	Acinetobacter baumannii D1279779	58.201	100	0.583
pilB	Acinetobacter baylyi ADP1	58.201	100	0.583
pilB	Legionella pneumophila strain ERS1305867	56.36	100	0.564
pilB	Vibrio cholerae strain A1552	51.601	99.293	0.512
pilB	Vibrio parahaemolyticus RIMD 2210633	50.089	99.47	0.498
pilB	Vibrio campbellii strain DS40M4	48.772	100	0.491
pilF	Neisseria gonorrhoeae MS11	49.378	99.47	0.491