Detailed information NTG0075629

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: KI237_RS18795
• Genome accession: NZ_AP021901
• Coordinates: 4265738..4266853 (-)
• Length: 371 a.a.
• NCBI ID: WP_212796517.1
• Uniprot ID: -
• Organism: Pseudomonas sp. St316
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 4260738..4271853

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
KI237_RS18780 (PHLH4_37530)	-	4261283..4262674 (+)	1392	WP_212796514.1	GntP family permease	-
KI237_RS18785 (PHLH4_37540)	hbdH	4262682..4263458 (+)	777	WP_212796515.1	3-hydroxybutyrate dehydrogenase	-
KI237_RS18790 (PHLH4_37550)	-	4263637..4265592 (+)	1956	WP_212796516.1	acetoacetate--CoA ligase	-
KI237_RS18795 (PHLH4_37560)	pilU	4265738..4266853 (-)	1116	WP_212796517.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
KI237_RS18800 (PHLH4_37570)	-	4266986..4269523 (+)	2538	WP_212796518.1	PAS domain-containing protein	-
KI237_RS18805 (PHLH4_37580)	-	4269572..4269847 (+)	276	WP_003182808.1	peptidylprolyl isomerase	-
KI237_RS18815 (PHLH4_37590)	-	4270150..4271073 (-)	924	WP_212796520.1	sugar kinase	-

Sequence

Protein

• Length: 371 a.a.
• Molecular weight: 41268.22 Da
• Isoelectric Point: 6.2401

>NTDB_id=75629 KI237_RS18795 WP_212796517.1 4265738..4266853(-) (pilU) [Pseudomonas sp. St316]
MEIDPLLRILASQNGSDLYLSTGAPPCARFEGVIKPLGNEAFKVGDIARLAESLMDAEQRLEFDRELEMNLAISLAGVGR
FRVNIFKQRNDVSMVIRNVKLDIPHFEDLKLPRVLLDTIMQKQGLMLFVGATGSGKSTSLAALIDYRNRNSSGHIITIED
PVEYIHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALAFADTGHLVISTLHAHNANQALDRV
INFFPEERRPQLLNDLGNNLKAFVSQRLVRTHTGQRRAAVEVMLGSPTVADLIRRNELGELKGIMEKSEELGMQTFDHAL
FNLVIEGAIDEEEALKNADSANNLRLRLKLHAESGAVPPADPAAGEWGLMD

Nucleotide

• Length: 1116 bp

>NTDB_id=75629 KI237_RS18795 WP_212796517.1 4265738..4266853(-) (pilU) [Pseudomonas sp. St316]
ATGGAAATCGATCCGTTGTTGCGAATCCTGGCGAGCCAGAATGGTTCCGACCTTTACCTGTCCACGGGCGCACCACCGTG
CGCGCGTTTCGAGGGCGTGATCAAGCCCTTGGGCAACGAAGCGTTCAAGGTCGGTGACATTGCCAGGCTCGCCGAGTCTC
TCATGGACGCCGAACAGCGCCTTGAGTTCGATCGGGAGTTGGAAATGAACCTGGCGATCTCCCTGGCCGGCGTCGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAATGACGTGTCCATGGTGATCCGCAACGTCAAGCTGGACATCCCGCACTTCGA
AGACCTGAAACTGCCTCGGGTGCTGTTGGACACCATCATGCAGAAACAGGGGCTGATGCTGTTCGTCGGTGCGACAGGCT
CAGGCAAGTCGACCTCCCTGGCGGCGCTGATCGATTACCGCAATCGCAACAGCAGCGGCCACATCATCACCATCGAGGAC
CCGGTGGAGTATATCCATCGGCACAAGAAATCCATCATCAATCAACGGGAAGTCGGTGTCGACACCCGCAGTTTCCATGC
TGCCCTGAAAAACACGCTGCGCCAGGCGCCGGACGTGGTGCTGATCGGCGAAATCCGTGACCGGGAAACCATGGAGCATG
CCTTGGCGTTTGCCGACACCGGGCACTTGGTGATTTCCACCTTGCATGCCCACAACGCCAACCAGGCCCTGGACCGGGTG
ATCAATTTTTTTCCGGAAGAACGCCGGCCGCAGTTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTCTCCCAGCG
TCTGGTGCGCACCCACACCGGCCAGCGGCGGGCGGCGGTGGAGGTCATGCTGGGCTCGCCGACGGTGGCGGACCTGATCC
GGCGCAACGAGCTCGGCGAGCTCAAGGGCATCATGGAGAAATCAGAGGAACTGGGGATGCAAACCTTCGATCATGCGCTG
TTCAACCTGGTGATCGAAGGCGCCATCGATGAGGAAGAGGCCCTGAAAAATGCCGACTCGGCGAACAACCTGCGGTTGCG
GTTGAAGTTGCATGCCGAGTCGGGCGCGGTGCCACCGGCCGATCCGGCGGCGGGTGAGTGGGGGCTTATGGATTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (79-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.338	95.687	0.539
pilU	Acinetobacter baylyi ADP1	52.247	95.957	0.501
pilU	Vibrio cholerae strain A1552	52.286	94.34	0.493
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	39.66	95.148	0.377
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	41.141	89.757	0.369
pilT	Vibrio cholerae strain A1552	41.141	89.757	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	92.722	0.367
pilT	Pseudomonas aeruginosa PAK	39.244	92.722	0.364
pilT	Acinetobacter nosocomialis M2	40.12	90.027	0.361
pilT	Acinetobacter baumannii D1279779	40.12	90.027	0.361
pilT	Acinetobacter baumannii strain A118	40.12	90.027	0.361