Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONZ57_RS15190 Genome accession   NZ_CP110654
Coordinates   3223514..3224653 (+) Length   379 a.a.
NCBI ID   WP_042467989.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain AS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3218514..3229653
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ57_RS15160 (ONZ57_15165) - 3218562..3218942 (-) 381 WP_005314618.1 diacylglycerol kinase -
  ONZ57_RS15165 (ONZ57_15170) - 3219097..3220158 (+) 1062 WP_017412107.1 3-deoxy-7-phosphoheptulonate synthase -
  ONZ57_RS15170 (ONZ57_15175) - 3220275..3221084 (-) 810 WP_005314614.1 ATP-binding cassette domain-containing protein -
  ONZ57_RS15175 (ONZ57_15180) btuC 3221144..3222151 (-) 1008 WP_005314605.1 vitamin B12 ABC transporter permease BtuC -
  ONZ57_RS15180 (ONZ57_15185) - 3222255..3222986 (-) 732 WP_005314604.1 substrate-binding periplasmic protein -
  ONZ57_RS15185 (ONZ57_15190) - 3223098..3223454 (+) 357 WP_005314602.1 DMT family protein -
  ONZ57_RS15190 (ONZ57_15195) pilU 3223514..3224653 (+) 1140 WP_042467989.1 type IVa pilus ATPase TapW Machinery gene
  ONZ57_RS15195 (ONZ57_15200) glgC 3224927..3226198 (+) 1272 WP_088814267.1 glucose-1-phosphate adenylyltransferase -
  ONZ57_RS15200 (ONZ57_15205) - 3226344..3226823 (+) 480 WP_017412110.1 WbuC family cupin fold metalloprotein -
  ONZ57_RS15205 (ONZ57_15210) - 3226919..3227161 (-) 243 WP_017412111.1 FeoC-like transcriptional regulator -
  ONZ57_RS15210 (ONZ57_15215) feoB 3227158..3229428 (-) 2271 WP_042467987.1 Fe(2+) transporter permease subunit FeoB -
  ONZ57_RS15215 (ONZ57_15220) - 3229425..3229652 (-) 228 WP_005314583.1 FeoA family protein -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42261.28 Da        Isoelectric Point: 6.4288

>NTDB_id=755756 ONZ57_RS15190 WP_042467989.1 3223514..3224653(+) (pilU) [Aeromonas salmonicida strain AS1]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNPLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=755756 ONZ57_RS15190 WP_042467989.1 3223514..3224653(+) (pilU) [Aeromonas salmonicida strain AS1]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
TGCCAAGTTCAACGGTGGGCTGCGCCCCCTGTCCGAGACGTCGCTCGAACCGGGTGAAGTAGCCAAGATCGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTGGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
GGATCTCAAACTGCCGCCCGTGTTGCTCGACACCATCATGGAGAAACGGGGCCTGGTGCTGTTTGTGGGCGGTACAGGCT
CGGGCAAATCCACGTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAACCCCCTGCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCACAGTTGCTCAACGATCTTGGCAACAACCTTAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAATTTGGCAGCCTGAAAGAGGTGATGGAGAAATCCAGAGCGCTCGGCATGGTCACCTTCGATAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAAGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAACTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGACG
AAAGCAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.895

100

0.53
  pilU Acinetobacter baylyi ADP1

52.958

93.668

0.496
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

99.736

0.404
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361