Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONZ66_RS08765 Genome accession   NZ_CP110647
Coordinates   1798932..1800071 (-) Length   379 a.a.
NCBI ID   WP_059113205.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain ZAS     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1793932..1805071
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ66_RS08745 (ONZ66_08745) feoB 1794154..1796424 (+) 2271 WP_271774683.1 Fe(2+) transporter permease subunit FeoB -
  ONZ66_RS08750 (ONZ66_08750) - 1796421..1796663 (+) 243 WP_021140137.1 FeoC-like transcriptional regulator -
  ONZ66_RS08755 (ONZ66_08755) - 1796761..1797240 (-) 480 WP_271774685.1 WbuC family cupin fold metalloprotein -
  ONZ66_RS08760 (ONZ66_08760) glgC 1797387..1798658 (-) 1272 WP_059113206.1 glucose-1-phosphate adenylyltransferase -
  ONZ66_RS08765 (ONZ66_08765) pilU 1798932..1800071 (-) 1140 WP_059113205.1 type IVa pilus ATPase TapW Machinery gene
  ONZ66_RS08770 (ONZ66_08770) - 1800131..1800487 (-) 357 WP_005314602.1 DMT family protein -
  ONZ66_RS08775 (ONZ66_08775) - 1800599..1801330 (+) 732 WP_059113204.1 substrate-binding periplasmic protein -
  ONZ66_RS08780 (ONZ66_08780) btuC 1801434..1802441 (+) 1008 WP_271774686.1 vitamin B12 ABC transporter permease BtuC -
  ONZ66_RS08785 (ONZ66_08785) - 1802501..1803310 (+) 810 WP_058394149.1 ATP-binding cassette domain-containing protein -
  ONZ66_RS08790 (ONZ66_08790) - 1803427..1804488 (-) 1062 WP_271774687.1 3-deoxy-7-phosphoheptulonate synthase -
  ONZ66_RS08795 (ONZ66_08795) - 1804643..1805023 (+) 381 WP_005314618.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42265.26 Da        Isoelectric Point: 6.4288

>NTDB_id=755650 ONZ66_RS08765 WP_059113205.1 1798932..1800071(-) (pilU) [Aeromonas salmonicida strain ZAS]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLIDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=755650 ONZ66_RS08765 WP_059113205.1 1798932..1800071(-) (pilU) [Aeromonas salmonicida strain ZAS]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
CGCCAAGTTCAACGGTGGGTTGCGCCCCCTGTCCGAGACGTCACTCGAACCGGGGGAAGTGGCCAAGATAGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGATCAACATCTTCAAGCAGCGCAACGAAGTCTCCCTGGTAGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
AGATCTCAAACTGCCCCCCGTGCTGATCGACACCATCATGGAGAAACGGGGCCTGGTGCTGTTCGTGGGCGGAACAGGCT
CGGGCAAATCCACGTCGCTTGCGGCCCTCATCGACCATCGAAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGAGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAACACCCTGCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAAACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCCGAGGAGCGCCGTCCCCAGCTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGCGAGTTTGGCAGCCTGAAAGAGGTGATGGAGAAATCCAGAGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAGGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATAAAACTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGATG
AAAGTAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(117-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.158

100

0.533
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.741

99.736

0.406
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361