Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   ONZ54_RS08830 Genome accession   NZ_CP110645
Coordinates   1796313..1797452 (-) Length   379 a.a.
NCBI ID   WP_011898593.1    Uniprot ID   A2T1B1
Organism   Aeromonas salmonicida strain AS3     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1791313..1802452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONZ54_RS08810 (ONZ54_08815) feoB 1791532..1793802 (+) 2271 WP_271787963.1 Fe(2+) transporter permease subunit FeoB -
  ONZ54_RS08815 (ONZ54_08820) - 1793799..1794041 (+) 243 WP_021140137.1 FeoC-like transcriptional regulator -
  ONZ54_RS08820 (ONZ54_08825) - 1794138..1794617 (-) 480 WP_271787964.1 WbuC family cupin fold metalloprotein -
  ONZ54_RS08825 (ONZ54_08830) glgC 1794768..1796039 (-) 1272 WP_017412109.1 glucose-1-phosphate adenylyltransferase -
  ONZ54_RS08830 (ONZ54_08835) pilU 1796313..1797452 (-) 1140 WP_011898593.1 type IVa pilus ATPase TapW Machinery gene
  ONZ54_RS08835 (ONZ54_08840) - 1797512..1797868 (-) 357 WP_005314602.1 DMT family protein -
  ONZ54_RS08840 (ONZ54_08845) - 1797980..1798711 (+) 732 WP_271787965.1 substrate-binding periplasmic protein -
  ONZ54_RS08845 (ONZ54_08850) btuC 1798815..1799822 (+) 1008 WP_058394150.1 vitamin B12 ABC transporter permease BtuC -
  ONZ54_RS08850 (ONZ54_08855) - 1799882..1800691 (+) 810 WP_271788265.1 ATP-binding cassette domain-containing protein -
  ONZ54_RS08855 (ONZ54_08860) - 1800776..1801840 (-) 1065 WP_271787966.1 3-deoxy-7-phosphoheptulonate synthase -
  ONZ54_RS08860 (ONZ54_08865) - 1801995..1802375 (+) 381 WP_058394146.1 diacylglycerol kinase -

Sequence

Protein


Download         Length: 379 a.a.        Molecular weight: 42265.26 Da        Isoelectric Point: 6.4288

>NTDB_id=755621 ONZ54_RS08830 WP_011898593.1 1796313..1797452(-) (pilU) [Aeromonas salmonicida strain AS3]
MELRDMLQILAKQDGSDLYLSTGAPPCAKFNGGLRPLSETSLEPGEVAKIAADIMDGEQKQQFERELEMNLAISLPQIGR
FRINIFKQRNEVSLVARNIKTEIPRFEDLKLPPVLLDTIMEKRGLVLFVGGTGSGKSTSLAALIDHRNRNSGGHIITIED
PVEFVHRHRKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFSETGHLAISTLHANNANQALDRI
INFFPEERRPQLLNDLGNNLKAFVSQRLVKTTDGGRRAAVEIMLGTHTIRDMIKRGEFGSLKEVMEKSRALGMVTFDSAL
FDLVVEGVIEEEEAVKNADSANNLRLKIKLWKEKGQIANSADATGWSLEPTKDESNDPF

Nucleotide


Download         Length: 1140 bp        

>NTDB_id=755621 ONZ54_RS08830 WP_011898593.1 1796313..1797452(-) (pilU) [Aeromonas salmonicida strain AS3]
ATGGAATTGCGAGACATGCTACAGATCCTGGCCAAGCAGGACGGCTCAGACCTCTATCTGTCGACCGGCGCCCCTCCCTG
TGCCAAGTTCAACGGTGGGCTGCGCCCCCTGTCCGAGACGTCGCTCGAACCGGGTGAAGTGGCCAAGATCGCCGCCGACA
TCATGGATGGCGAGCAGAAGCAGCAGTTCGAGCGGGAGCTGGAGATGAACCTCGCCATCTCCCTGCCCCAGATAGGCCGT
TTTCGGATCAACATCTTCAAGCAGCGCAATGAAGTCTCCCTGGTGGCCCGCAACATCAAGACAGAGATCCCCCGCTTTGA
AGATCTCAAACTGCCCCCCGTGCTGCTCGACACCATCATGGAGAAACGGGGCCTAGTGCTGTTTGTGGGCGGAACAGGCT
CGGGCAAATCCACGTCGCTGGCGGCCCTCATCGACCATCGCAACCGCAACAGCGGCGGCCACATCATCACCATCGAGGAT
CCGGTGGAGTTCGTCCATCGTCACCGCAAGAGCATCATCAACCAGCGGGAGGTGGGGGTAGATACCCGCAGCTTCCACGC
CGCGCTCAAGAACACCCTGCGCCAGGCACCGGATGTGATCCTCATCGGTGAAATTCGCGATCGCGAGACCATGGAGCATG
CCCTTGCCTTCTCCGAGACCGGGCATCTGGCCATCTCCACCCTGCACGCCAACAACGCCAACCAGGCGCTGGATCGCATC
ATCAACTTCTTCCCGGAAGAGCGCCGGCCGCAACTGCTCAACGATCTTGGCAACAACCTCAAGGCCTTTGTCTCCCAGCG
ACTGGTGAAAACCACAGACGGTGGCCGCCGGGCGGCGGTGGAGATCATGCTGGGCACCCACACCATCCGCGACATGATCA
AACGCGGTGAATTTGGCAGCCTGAAAGAGGTGATGGAAAAATCCAGAGCGCTCGGCATGGTCACCTTCGACAGCGCCCTG
TTCGATCTGGTGGTGGAAGGGGTGATAGAGGAGGAAGAAGCGGTGAAAAACGCCGACTCGGCCAACAACCTGCGCCTCAA
GATCAAGTTGTGGAAAGAGAAGGGCCAGATCGCCAACAGTGCGGATGCCACCGGCTGGAGTCTGGAGCCCACCAAGGATG
AAAGTAACGATCCCTTCTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(114-248)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T1B1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

53.158

100

0.533
  pilU Acinetobacter baylyi ADP1

53.239

93.668

0.499
  pilU Vibrio cholerae strain A1552

50

92.348

0.462
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.476

99.736

0.404
  pilT Legionella pneumophila strain Lp02

42.216

88.127

0.372
  pilT Legionella pneumophila strain ERS1305867

42.216

88.127

0.372
  pilT Pseudomonas aeruginosa PAK

40.698

90.765

0.369
  pilT Pseudomonas stutzeri DSM 10701

40.407

90.765

0.367
  pilT Acinetobacter baumannii strain A118

41.018

88.127

0.361
  pilT Acinetobacter baumannii D1279779

41.018

88.127

0.361
  pilT Acinetobacter nosocomialis M2

41.018

88.127

0.361