Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGC/cglC   Type   Machinery gene
Locus tag   OLL94_RS05480 Genome accession   NZ_CP110071
Coordinates   1194856..1195131 (+) Length   91 a.a.
NCBI ID   WP_002356991.1    Uniprot ID   A0A1B4XPV0
Organism   Enterococcus faecalis strain BE8     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1195155..1244031 1194856..1195131 flank 24


Gene organization within MGE regions


Location: 1194856..1244031
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLL94_RS05480 (OLL94_05480) comGC/cglC 1194856..1195131 (+) 276 WP_002356991.1 competence type IV pilus major pilin ComGC Machinery gene
  OLL94_RS05485 (OLL94_05485) comGD 1195128..1195562 (+) 435 Protein_1077 competence type IV pilus minor pilin ComGD -
  OLL94_RS05490 (OLL94_05490) - 1195599..1196747 (-) 1149 WP_002378469.1 site-specific integrase -
  OLL94_RS05495 (OLL94_05495) - 1196847..1197575 (-) 729 WP_002378468.1 ion transporter -
  OLL94_RS05500 (OLL94_05500) - 1197611..1197955 (-) 345 WP_002378467.1 ImmA/IrrE family metallo-endopeptidase -
  OLL94_RS05505 (OLL94_05505) - 1197972..1198304 (-) 333 WP_002364355.1 helix-turn-helix domain-containing protein -
  OLL94_RS05510 (OLL94_05510) - 1198616..1198792 (+) 177 WP_002364354.1 helix-turn-helix transcriptional regulator -
  OLL94_RS05515 (OLL94_05515) - 1198803..1199114 (+) 312 WP_002381719.1 hypothetical protein -
  OLL94_RS05520 (OLL94_05520) - 1199153..1199878 (+) 726 WP_002378466.1 phage regulatory protein -
  OLL94_RS05525 (OLL94_05525) - 1199904..1200092 (-) 189 WP_002357001.1 YegP family protein -
  OLL94_RS05530 (OLL94_05530) - 1200147..1200356 (+) 210 WP_002378465.1 hypothetical protein -
  OLL94_RS05535 (OLL94_05535) - 1200393..1200587 (+) 195 WP_002378464.1 hypothetical protein -
  OLL94_RS05540 (OLL94_05540) - 1201014..1201568 (-) 555 WP_002357006.1 hypothetical protein -
  OLL94_RS05545 (OLL94_05545) - 1201788..1202105 (+) 318 WP_002357007.1 hypothetical protein -
  OLL94_RS05550 (OLL94_05550) - 1202098..1202832 (+) 735 WP_002378463.1 ERF family protein -
  OLL94_RS05555 (OLL94_05555) - 1202837..1203478 (+) 642 WP_002364344.1 putative HNHc nuclease -
  OLL94_RS05560 (OLL94_05560) - 1203483..1203683 (+) 201 WP_002357010.1 hypothetical protein -
  OLL94_RS05565 (OLL94_05565) - 1203683..1204540 (+) 858 WP_002364343.1 helix-turn-helix domain-containing protein -
  OLL94_RS05570 (OLL94_05570) - 1204537..1204944 (+) 408 WP_002378462.1 RusA family crossover junction endodeoxyribonuclease -
  OLL94_RS05575 (OLL94_05575) - 1205852..1206268 (+) 417 WP_002357018.1 ArpU family phage packaging/lysis transcriptional regulator -
  OLL94_RS05585 (OLL94_05585) - 1206983..1207891 (+) 909 WP_002378460.1 hypothetical protein -
  OLL94_RS05590 (OLL94_05590) - 1208008..1208370 (+) 363 WP_224800088.1 hypothetical protein -
  OLL94_RS05595 (OLL94_05595) - 1208841..1209071 (+) 231 Protein_1098 hypothetical protein -
  OLL94_RS05600 (OLL94_05600) - 1209103..1209537 (+) 435 WP_002364295.1 terminase small subunit -
  OLL94_RS05605 (OLL94_05605) - 1209518..1210801 (+) 1284 WP_002369892.1 PBSX family phage terminase large subunit -
  OLL94_RS05610 (OLL94_05610) - 1210801..1212285 (+) 1485 WP_002378458.1 phage portal protein -
  OLL94_RS05615 (OLL94_05615) - 1212278..1213204 (+) 927 WP_002378457.1 minor capsid protein -
  OLL94_RS05620 (OLL94_05620) - 1213327..1213962 (+) 636 WP_010710204.1 DUF4355 domain-containing protein -
  OLL94_RS05625 (OLL94_05625) - 1213976..1214872 (+) 897 WP_002378455.1 hypothetical protein -
  OLL94_RS05630 (OLL94_05630) - 1214943..1215275 (+) 333 WP_002363369.1 phage head-tail connector protein -
  OLL94_RS05635 (OLL94_05635) - 1215272..1215547 (+) 276 WP_002378454.1 hypothetical protein -
  OLL94_RS05640 (OLL94_05640) - 1215544..1215882 (+) 339 WP_002363372.1 HK97-gp10 family putative phage morphogenesis protein -
  OLL94_RS05645 (OLL94_05645) - 1215879..1216271 (+) 393 WP_002363373.1 DUF3168 domain-containing protein -
  OLL94_RS05650 (OLL94_05650) - 1216290..1216895 (+) 606 WP_002364302.1 phage major tail protein, TP901-1 family -
  OLL94_RS05655 (OLL94_05655) - 1216898..1217080 (+) 183 WP_002378453.1 hypothetical protein -
  OLL94_RS05660 (OLL94_05660) - 1217129..1217701 (+) 573 WP_002401805.1 immunoglobulin-like domain-containing protein -
  OLL94_RS05665 (OLL94_05665) - 1217755..1218153 (+) 399 WP_002378449.1 tail assembly chaperone -
  OLL94_RS05670 (OLL94_05670) - 1218222..1218527 (+) 306 WP_002366371.1 hypothetical protein -
  OLL94_RS05675 (OLL94_05675) - 1218514..1222968 (+) 4455 WP_002378448.1 phage tail tape measure protein -
  OLL94_RS05680 (OLL94_05680) - 1222965..1223687 (+) 723 WP_002363380.1 hypothetical protein -
  OLL94_RS05685 (OLL94_05685) - 1223687..1225132 (+) 1446 WP_002378447.1 phage tail spike protein -
  OLL94_RS05690 (OLL94_05690) - 1225138..1225878 (+) 741 WP_002363383.1 hypothetical protein -
  OLL94_RS05695 (OLL94_05695) - 1225895..1226347 (+) 453 WP_002363384.1 hypothetical protein -
  OLL94_RS05700 (OLL94_05700) - 1226366..1226761 (+) 396 WP_002363385.1 hypothetical protein -
  OLL94_RS05705 (OLL94_05705) - 1226763..1226885 (+) 123 WP_002368228.1 XkdX family protein -
  OLL94_RS05710 (OLL94_05710) - 1226896..1227276 (+) 381 WP_002378446.1 phage holin family protein -
  OLL94_RS05715 (OLL94_05715) - 1227289..1228548 (+) 1260 WP_002378445.1 LysM peptidoglycan-binding domain-containing protein -
  OLL94_RS05720 (OLL94_05720) - 1229401..1229601 (+) 201 WP_002357053.1 cold-shock protein -
  OLL94_RS05725 (OLL94_05725) - 1229673..1229945 (+) 273 WP_002378444.1 hypothetical protein -
  OLL94_RS05735 (OLL94_05735) hemH 1230661..1231602 (+) 942 WP_002357056.1 ferrochelatase -
  OLL94_RS05740 (OLL94_05740) - 1232403..1232729 (+) 327 WP_002370967.1 type II secretion system protein -
  OLL94_RS05745 (OLL94_05745) comGF 1232719..1233153 (+) 435 WP_002378441.1 competence type IV pilus minor pilin ComGF -
  OLL94_RS05750 (OLL94_05750) comGG 1233153..1233506 (+) 354 WP_002362054.1 competence type IV pilus minor pilin ComGG -
  OLL94_RS05755 (OLL94_05755) - 1233635..1234642 (+) 1008 WP_002357063.1 class I SAM-dependent methyltransferase -
  OLL94_RS05760 (OLL94_05760) - 1234667..1235854 (+) 1188 WP_002357064.1 acetate/propionate family kinase -
  OLL94_RS05765 (OLL94_05765) - 1235966..1236439 (-) 474 WP_002357065.1 universal stress protein -
  OLL94_RS05770 (OLL94_05770) - 1236755..1237087 (-) 333 WP_002357068.1 hypothetical protein -
  OLL94_RS05780 (OLL94_05780) - 1237361..1238638 (-) 1278 WP_002360030.1 replication-associated recombination protein A -
  OLL94_RS05785 (OLL94_05785) - 1238767..1239444 (+) 678 WP_002364174.1 DNA-3-methyladenine glycosylase -
  OLL94_RS05790 (OLL94_05790) - 1239401..1239886 (+) 486 WP_002388170.1 DUF3013 family protein -
  OLL94_RS05795 (OLL94_05795) prmA 1239902..1240849 (+) 948 WP_002371999.1 50S ribosomal protein L11 methyltransferase -
  OLL94_RS05800 (OLL94_05800) - 1240851..1241603 (+) 753 WP_002378439.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -
  OLL94_RS05805 (OLL94_05805) - 1241818..1244031 (+) 2214 WP_002357079.1 bifunctional (p)ppGpp synthetase/guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase -

Sequence

Protein


Download         Length: 91 a.a.        Molecular weight: 10464.41 Da        Isoelectric Point: 9.3192

>NTDB_id=752042 OLL94_RS05480 WP_002356991.1 1194856..1195131(+) (comGC/cglC) [Enterococcus faecalis strain BE8]
MKKKQKYAGFTLLEMLIVLLIISVLILLFVPNLAKHKETVDKKGNEAIVKIVESQIELYTLEKNKTPSLNELVNEGYITK
EQLDKYTAEKQ

Nucleotide


Download         Length: 276 bp        

>NTDB_id=752042 OLL94_RS05480 WP_002356991.1 1194856..1195131(+) (comGC/cglC) [Enterococcus faecalis strain BE8]
ATGAAAAAGAAACAAAAATACGCAGGGTTTACATTATTAGAAATGTTGATTGTCTTATTGATTATTTCCGTATTGATTTT
ACTTTTTGTTCCTAACTTAGCGAAACATAAAGAAACAGTTGATAAAAAAGGCAATGAAGCAATCGTAAAAATTGTAGAAT
CACAAATCGAGCTCTACACACTAGAAAAAAATAAGACGCCTTCCTTAAATGAATTAGTCAACGAAGGCTACATTACTAAA
GAGCAGTTAGATAAATATACAGCAGAAAAGCAATGA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1B4XPV0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGC/cglC Streptococcus pneumoniae Rx1

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae D39

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae R6

63.095

92.308

0.582
  comGC/cglC Streptococcus pneumoniae TIGR4

63.095

92.308

0.582
  comGC/cglC Streptococcus mitis NCTC 12261

61.905

92.308

0.571
  comGC/cglC Streptococcus mitis SK321

61.176

93.407

0.571
  comGC Lactococcus lactis subsp. cremoris KW2

58.14

94.505

0.549
  comYC Streptococcus gordonii str. Challis substr. CH1

56.322

95.604

0.538
  comYC Streptococcus suis isolate S10

52.326

94.505

0.495
  comYC Streptococcus mutans UA159

57.692

85.714

0.495
  comYC Streptococcus mutans UA140

57.692

85.714

0.495
  comGC Latilactobacillus sakei subsp. sakei 23K

46.154

100

0.462
  comGC Staphylococcus aureus MW2

46.835

86.813

0.407
  comGC Staphylococcus aureus N315

46.835

86.813

0.407
  comGC Bacillus subtilis subsp. subtilis str. 168

48.649

81.319

0.396