Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   K5706_RS01625 Genome accession   NZ_AP019849
Coordinates   340927..342606 (+) Length   559 a.a.
NCBI ID   WP_420883200.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 335927..347606
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5706_RS01605 (VA249_02670) pilM 338210..339223 (+) 1014 WP_221070791.1 type IV pilus biogenesis protein PilM Machinery gene
  K5706_RS01610 (VA249_02680) pilN 339207..339782 (+) 576 WP_221070793.1 PilN domain-containing protein Machinery gene
  K5706_RS01615 (VA249_02690) pilO 339775..340368 (+) 594 WP_221070794.1 type 4a pilus biogenesis protein PilO Machinery gene
  K5706_RS01620 (VA249_02700) pilP 340358..340867 (+) 510 WP_221070797.1 pilus assembly protein PilP Machinery gene
  K5706_RS01625 (VA249_02710) pilQ 340927..342606 (+) 1680 WP_420883200.1 type IV pilus secretin PilQ Machinery gene
  K5706_RS01630 (VA249_02720) aroK 342795..343313 (+) 519 WP_099080818.1 shikimate kinase AroK -
  K5706_RS01635 (VA249_02730) aroB 343342..344442 (+) 1101 WP_221068216.1 3-dehydroquinate synthase -
  K5706_RS01640 (VA249_02740) - 344494..345537 (+) 1044 Protein_272 ATP-binding protein -
  K5706_RS24090 - 345619..346008 (+) 390 Protein_273 SPOR domain-containing protein -
  K5706_RS01645 (VA249_02750) - 346078..346917 (+) 840 WP_221068214.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 61490.16 Da        Isoelectric Point: 4.6087

>NTDB_id=74381 K5706_RS01625 WP_420883200.1 340927..342606(+) (pilQ) [Vibrio alfacsensis strain 04Ya249]
MVVGFFSVWLFMFLASEGRAKASPPNELKSIDFRADNNTEAVIMIELTSSSVVVDIQRERDGLSIELLNTSVSDNQLHLL
DVRDFSTVIKQIDVSRYHSATRLLAYTTDEYRYDYRLSGRWLEVTVVKEKSTKQAQSQNILKNKGRLISINFQDIPVRNV
LQLIADYNDFNLVVSDSVSGNLTLRLDDVPWPQVLDIILQVKGLDKRVDGNVILIAPLDELELREKQRLEKQKLEQEIGE
LSSEIIKVNFAKASDVAEMINGEGNISMLSERGSMTVDERTNSLLVRELPENIDVIREIIEALDIPVKQVQIEARIVTIN
EGNLDELGVRWGFTSINGSSTVGGSIENNLATLGLYDGGVGIDDFLNVNLAATNPSATSLAFQVAKLGAGTLLDLELSAL
QQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATSVTFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGQVVK
TGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSLSNAVDKVPLLGDLPLLGALFRRSYEKVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=74381 K5706_RS01625 WP_420883200.1 340927..342606(+) (pilQ) [Vibrio alfacsensis strain 04Ya249]
ATGGTGGTTGGTTTTTTCTCTGTGTGGCTATTTATGTTTTTGGCATCAGAGGGGAGAGCAAAGGCGTCACCACCGAATGA
ATTAAAATCGATAGACTTCCGTGCAGATAACAATACAGAAGCGGTGATCATGATTGAGCTAACGTCATCGTCTGTCGTGG
TTGATATACAACGTGAAAGAGATGGGTTAAGTATTGAGCTGCTTAATACTTCGGTCAGTGATAACCAACTGCACCTTTTG
GACGTGAGAGATTTCTCTACCGTCATTAAGCAAATTGACGTTTCTCGTTATCATTCGGCCACGCGTTTGTTGGCCTATAC
GACCGATGAATACCGCTATGACTATCGCCTGTCAGGACGCTGGTTAGAAGTGACAGTGGTGAAAGAAAAGTCAACTAAGC
AAGCACAAAGCCAGAACATTTTGAAGAATAAAGGGCGTTTAATCTCTATCAATTTTCAAGATATTCCGGTGCGAAATGTA
TTGCAGTTAATTGCTGACTATAACGATTTCAATCTCGTGGTATCGGATTCTGTGTCGGGTAATCTGACGTTACGCTTAGA
TGACGTACCTTGGCCACAAGTGTTAGATATCATTTTGCAAGTGAAAGGATTGGATAAACGGGTTGATGGCAATGTTATTT
TGATTGCCCCATTAGATGAACTGGAGCTAAGAGAAAAGCAGCGTCTTGAAAAGCAAAAGCTTGAGCAAGAAATAGGAGAG
CTTTCCTCTGAGATCATCAAAGTGAATTTTGCAAAAGCCTCGGACGTTGCGGAAATGATCAATGGCGAGGGTAATATCAG
CATGCTTTCTGAGAGGGGAAGTATGACCGTTGATGAGCGTACGAATTCATTGTTGGTGCGAGAATTACCAGAGAACATTG
ACGTTATTCGCGAAATTATAGAAGCGTTAGACATTCCCGTGAAACAAGTACAGATTGAAGCTCGCATTGTCACCATTAAT
GAGGGGAATCTCGATGAGCTTGGGGTACGCTGGGGTTTTACAAGTATAAATGGCAGTTCAACGGTTGGAGGCTCGATTGA
AAATAATCTCGCCACGCTTGGTTTGTATGATGGTGGCGTTGGCATTGATGATTTTCTTAATGTTAACCTTGCAGCGACCA
ACCCAAGTGCCACTAGCCTCGCTTTTCAAGTCGCAAAGTTAGGGGCAGGGACTTTGCTTGATTTAGAGCTGTCTGCGTTG
CAGCAGGAGTCAAAAGCAGAAATTATCTCGAGTCCACGGTTGATCACCACCAATAAGAAACCCGCCTATATTGAGCAGGG
AACGGAAATCCCGTATTTAGAATCGTCATCAAGTGGGGCGACGTCGGTGACATTTAAAAAAGCAGTATTGAGTTTAAAAG
TTACGCCTCAGATAACGCCAGATAATCGCTTGGTGTTGGATTTGAGTGTAACTCAAGACCGGCCTGGTCAAGTGGTGAAA
ACAGGCACGGGAGAAGCGGTTGCAATTGATACTCAGAGAATTGGCACTCAAGTTTTGGTCAATAATGGTGAAACGGTGGT
ATTGGGTGGTATTTTCCAACATAGCCTCAGTAATGCCGTTGACAAAGTCCCTTTACTTGGTGATTTACCCTTGCTTGGCG
CGCTTTTTCGCCGAAGTTATGAAAAAGTAGGCAAAAGTGAGTTGTTGATTTTCGTGACGCCAAAGGTCGTAATTCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(170-217)

Bacterial type II and III secretion system protein (Secretin)

(399-557)

Bacterial type II/III secretion system short domain (Secretin_N)

(244-309)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

82.505

97.138

0.801
  pilQ Vibrio cholerae strain A1552

71.02

100

0.71
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

71.02

100

0.71
  pilQ Pseudomonas aeruginosa PAK

40.502

99.821

0.404
  pilQ Legionella pneumophila strain ERS1305867

40.775

96.959

0.395
  pilQ Legionella pneumophila strain Lp02

40.775

96.959

0.395
  comQ Acinetobacter baylyi ADP1

38.801

100

0.394
  pilQ Acinetobacter baumannii strain A118

36.082

100

0.376
  pilQ Acinetobacter baumannii D1279779

36.082

100

0.376

Multiple sequence alignment