Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   K5706_RS01605 Genome accession   NZ_AP019849
Coordinates   338210..339223 (+) Length   337 a.a.
NCBI ID   WP_221070791.1    Uniprot ID   -
Organism   Vibrio alfacsensis strain 04Ya249     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 333210..344223
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K5706_RS01590 (VA249_02640) - 333613..334341 (-) 729 WP_221070786.1 glutathione peroxidase -
  K5706_RS01595 (VA249_02650) oxyR 334514..335422 (+) 909 WP_221068222.1 DNA-binding transcriptional regulator OxyR -
  K5706_RS01600 (VA249_02660) - 335504..338068 (-) 2565 WP_221070788.1 penicillin-binding protein 1A -
  K5706_RS01605 (VA249_02670) pilM 338210..339223 (+) 1014 WP_221070791.1 type IV pilus biogenesis protein PilM Machinery gene
  K5706_RS01610 (VA249_02680) pilN 339207..339782 (+) 576 WP_221070793.1 PilN domain-containing protein Machinery gene
  K5706_RS01615 (VA249_02690) pilO 339775..340368 (+) 594 WP_221070794.1 type 4a pilus biogenesis protein PilO Machinery gene
  K5706_RS01620 (VA249_02700) pilP 340358..340867 (+) 510 WP_221070797.1 pilus assembly protein PilP Machinery gene
  K5706_RS01625 (VA249_02710) pilQ 340927..342606 (+) 1680 WP_420883200.1 type IV pilus secretin PilQ Machinery gene
  K5706_RS01630 (VA249_02720) aroK 342795..343313 (+) 519 WP_099080818.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 337 a.a.        Molecular weight: 37664.06 Da        Isoelectric Point: 7.0389

>NTDB_id=74377 K5706_RS01605 WP_221070791.1 338210..339223(+) (pilM) [Vibrio alfacsensis strain 04Ya249]
MDKLVVTGIDIGHNSLKAVVLKPSGRHYAVLDYKEIILKECIVAENNTINHQEIVKTLKEIKKALPFGAKRVAISVPENA
VISKKLQIDPGLDSSERDYAVMQVFAHQSPFPLQELSLDFVLDDVKNKTDGLEHYQVFATRKDVVDSRVDALQQSGLKPV
LVEVASQSLGHMWKLATEHFPEKSTYCLLNVGNVKSSFTMFTEQGELFHKEFSSSSWSEKTPSVYQKGACIQVDESHQEQ
ALIERVQRQLQLYASFNGSLNITGIWITGDGFVTPQLKRALSSQLSMECETLNPFELFTLKASLHKHKAVQSHQFTAAAG
LAIRGIHWLEHGNVVSH

Nucleotide


Download         Length: 1014 bp        

>NTDB_id=74377 K5706_RS01605 WP_221070791.1 338210..339223(+) (pilM) [Vibrio alfacsensis strain 04Ya249]
ATGGACAAACTCGTCGTTACTGGCATCGACATAGGCCATAACAGTCTCAAAGCCGTGGTACTTAAGCCTAGTGGTCGCCA
TTACGCGGTACTGGACTACAAAGAAATAATATTGAAAGAATGTATTGTCGCTGAAAATAACACCATAAATCATCAAGAAA
TTGTAAAGACACTTAAAGAGATAAAAAAAGCACTGCCTTTCGGGGCTAAACGTGTCGCGATTTCGGTGCCAGAAAATGCC
GTGATCAGCAAAAAACTGCAAATAGATCCAGGGTTAGATAGCAGTGAACGAGACTATGCGGTCATGCAAGTTTTCGCTCA
TCAATCACCTTTCCCCTTACAAGAGCTTAGTTTAGATTTTGTTTTAGATGATGTGAAGAATAAAACGGATGGACTTGAAC
ATTATCAAGTATTTGCTACTCGTAAGGACGTTGTTGATTCTCGAGTCGATGCTTTACAACAATCAGGGTTAAAGCCTGTG
TTAGTAGAGGTGGCATCACAAAGCTTAGGTCATATGTGGAAGCTCGCGACGGAGCATTTTCCAGAGAAAAGTACCTACTG
CTTGCTCAATGTCGGCAACGTTAAATCATCGTTCACAATGTTTACTGAACAGGGTGAATTATTTCACAAAGAGTTCTCAT
CAAGTTCATGGTCTGAAAAGACGCCATCAGTATATCAGAAAGGTGCTTGTATACAGGTGGATGAATCACACCAAGAGCAA
GCTCTGATTGAGCGAGTTCAGCGTCAACTGCAGCTTTATGCTTCTTTCAATGGTTCTCTCAATATTACCGGTATCTGGAT
TACAGGTGACGGTTTTGTCACACCCCAATTAAAGAGAGCGTTATCGAGTCAATTGAGCATGGAATGTGAAACACTCAACC
CGTTCGAGTTGTTTACATTGAAGGCGTCACTGCATAAGCATAAGGCTGTTCAATCGCACCAGTTTACTGCTGCTGCGGGC
CTCGCGATTCGAGGTATTCATTGGTTGGAGCATGGCAATGTTGTATCGCATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-211)

Type IV pilus assembly protein PilM; (PilM_2)

(240-325)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

63.235

100

0.638
  pilM Vibrio cholerae strain A1552

41.765

100

0.421

Multiple sequence alignment