Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VroAM7_RS14715 Genome accession   NZ_AP019798
Coordinates   3126914..3128647 (-) Length   577 a.a.
NCBI ID   WP_143693016.1    Uniprot ID   A0A510I8S0
Organism   Vibrio rotiferianus strain AM7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3121914..3133647
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VroAM7_RS14695 (VroAM7_27380) - 3122594..3123433 (-) 840 WP_143693014.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VroAM7_RS14700 (VroAM7_27390) - 3123503..3125023 (-) 1521 WP_143693015.1 SPOR domain-containing protein -
  VroAM7_RS14705 (VroAM7_27400) aroB 3125075..3126175 (-) 1101 WP_010443901.1 3-dehydroquinate synthase -
  VroAM7_RS14710 (VroAM7_27410) aroK 3126207..3126725 (-) 519 WP_010443904.1 shikimate kinase AroK -
  VroAM7_RS14715 (VroAM7_27420) pilQ 3126914..3128647 (-) 1734 WP_143693016.1 type IV pilus secretin PilQ Machinery gene
  VroAM7_RS14720 (VroAM7_27430) pilP 3128685..3129197 (-) 513 WP_088880304.1 pilus assembly protein PilP Machinery gene
  VroAM7_RS14725 (VroAM7_27440) pilO 3129187..3129780 (-) 594 WP_143693017.1 type 4a pilus biogenesis protein PilO Machinery gene
  VroAM7_RS14730 (VroAM7_27450) pilN 3129773..3130360 (-) 588 WP_143693018.1 PilN domain-containing protein Machinery gene
  VroAM7_RS14735 (VroAM7_27460) pilM 3130344..3131360 (-) 1017 WP_138941723.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 63417.38 Da        Isoelectric Point: 4.4923

>NTDB_id=74140 VroAM7_RS14715 WP_143693016.1 3126914..3128647(-) (pilQ) [Vibrio rotiferianus strain AM7]
MKQGNTLRYRKLLELLWLCGLLLFPPAVWAEESQSNALRNIDFRSNKNKEAVIVVELASPAAIVDVKRVQEGLSIDLLST
SVKDDQLYLLDVKDFATAVDSVEVFRDAATTRLLAHIEGDYTHDYRLTGRYLEVKVARLKPNEKVPDKSILEKEGKLISI
NFQDIPVRNVLQLIADYNDFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQLE
KQKLEEEIGELASEIIKVNFAKASDIAEMINGEGNISMLSNRGSMTIDERTNSLLIRELPENIEVIRDIVESLDIPVKQV
QIEARIVTVNEGNLDELGVRWGFSSINGNNMVGGSIENNLAAIGLYDEGGDNEDGNTVGIDDFLNVNLAATSPSATSIAF
QVAKLGSDTLLDLELSALQQESKAEIISSPRLVTTNKKPAYIEQGTEIPYLEASSSGATSITFKKAVLSLKVTPQITPDN
RLVLDLSVTQDRLGKVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHNVTNTVDKVPLLGDLPLLGALFRRSYEN
VGKSELLIFVTPKVVVQ

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=74140 VroAM7_RS14715 WP_143693016.1 3126914..3128647(-) (pilQ) [Vibrio rotiferianus strain AM7]
ATGAAACAAGGAAATACATTGAGATACAGGAAGTTATTAGAACTCTTATGGTTGTGTGGACTACTATTGTTTCCCCCAGC
TGTTTGGGCTGAGGAGTCTCAGTCTAACGCCCTGAGAAATATTGATTTCAGAAGTAACAAAAATAAAGAGGCGGTCATTG
TTGTTGAGCTCGCATCGCCTGCCGCTATCGTTGATGTAAAACGAGTTCAAGAAGGGTTGAGTATCGACCTTCTTAGTACG
TCTGTGAAAGATGATCAACTTTACCTTCTCGATGTCAAAGACTTTGCAACTGCCGTCGATAGTGTTGAAGTCTTCCGTGA
TGCTGCAACAACTCGGTTATTGGCGCACATTGAAGGGGACTATACACATGATTACAGGCTTACTGGGCGATATCTCGAAG
TCAAAGTTGCACGCTTAAAACCGAATGAAAAGGTGCCAGATAAAAGTATCCTTGAGAAGGAAGGCAAGCTCATCTCTATT
AACTTTCAAGATATACCGGTAAGAAATGTCTTGCAGTTGATCGCTGACTACAACGATTTTAATTTGGTGGTCTCGGATTC
TGTTGAGGGTAACTTAACGTTACGCCTGGATGGTGTGCCATGGCAGCAGGTACTAGACATTATTCTACAGGTTAAGGGAC
TTGATAAACGCGTTGATGGCAACGTTATTTTAGTCGCACCAAAAGAAGAACTGGACTTACGTGAAAAGCAGCAATTGGAG
AAGCAAAAATTAGAAGAGGAAATTGGAGAGCTAGCATCAGAAATCATTAAAGTAAATTTTGCAAAAGCTTCGGATATTGC
GGAGATGATTAATGGTGAAGGGAATATTAGTATGCTTTCCAATCGAGGTAGTATGACAATCGATGAGCGCACTAACTCTC
TGCTTATTCGTGAGCTGCCAGAGAATATCGAAGTAATACGTGACATCGTAGAGTCACTAGATATACCCGTCAAGCAGGTT
CAAATAGAGGCCAGAATTGTAACCGTGAATGAAGGCAACCTTGATGAACTTGGTGTTCGCTGGGGCTTTAGTTCCATAAA
TGGTAACAACATGGTAGGTGGTTCGATTGAGAACAATCTTGCTGCTATTGGCCTTTATGACGAAGGTGGTGATAATGAAG
ATGGTAATACCGTTGGTATTGATGATTTCTTAAATGTAAACCTAGCGGCGACTTCGCCTAGCGCAACGAGTATTGCCTTT
CAAGTTGCTAAGTTGGGCTCTGATACGCTGTTAGATCTGGAGCTTTCCGCTCTACAACAAGAATCTAAGGCTGAAATCAT
CTCAAGTCCGCGTTTGGTAACGACAAACAAAAAGCCTGCCTATATCGAGCAAGGTACAGAAATTCCTTATCTCGAAGCAT
CGTCCAGCGGGGCGACTTCCATCACTTTCAAAAAAGCAGTATTAAGTCTAAAAGTCACGCCACAAATAACCCCAGACAAT
CGTTTAGTTCTAGATCTGAGTGTAACCCAAGATCGTTTAGGTAAGGTTGTCAAAACAGGTACTGGGGAAGCCGTTGCAAT
TGATACGCAGCGCATAGGAACTCAAGTATTAGTCAATAATGGTGAAACCGTCGTTTTAGGTGGAATATTTCAGCACAATG
TGACTAATACTGTGGACAAGGTTCCTCTGCTTGGAGATCTCCCATTGCTAGGGGCGTTATTTCGCAGAAGTTATGAAAAT
GTGGGTAAGAGTGAGCTACTGATCTTTGTTACACCTAAGGTAGTAGTTCAGTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(254-319)

Bacterial type II and III secretion system protein (Secretin)

(417-575)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510I8S0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

87.024

100

0.872
  pilQ Vibrio cholerae strain A1552

74.74

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.74

100

0.749
  pilQ Pseudomonas aeruginosa PAK

42.134

95.841

0.404
  pilQ Legionella pneumophila strain Lp02

40.325

95.841

0.386
  pilQ Legionella pneumophila strain ERS1305867

40.325

95.841

0.386
  comQ Acinetobacter baylyi ADP1

38.235

100

0.383
  pilQ Acinetobacter baumannii strain A118

37.075

100

0.378
  pilQ Acinetobacter baumannii D1279779

37.075

100

0.378

Multiple sequence alignment