Detailed information NTG0074144

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: VroAM7_RS14735
• Genome accession: NZ_AP019798
• Coordinates: 3130344..3131360 (-)
• Length: 338 a.a.
• NCBI ID: WP_138941723.1
• Uniprot ID: A0A510I8N2
• Organism: Vibrio rotiferianus strain AM7
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3125344..3136360

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
VroAM7_RS14710 (VroAM7_27410)	aroK	3126207..3126725 (-)	519	WP_010443904.1	shikimate kinase AroK	-
VroAM7_RS14715 (VroAM7_27420)	pilQ	3126914..3128647 (-)	1734	WP_143693016.1	type IV pilus secretin PilQ	Machinery gene
VroAM7_RS14720 (VroAM7_27430)	pilP	3128685..3129197 (-)	513	WP_088880304.1	pilus assembly protein PilP	Machinery gene
VroAM7_RS14725 (VroAM7_27440)	pilO	3129187..3129780 (-)	594	WP_143693017.1	type 4a pilus biogenesis protein PilO	Machinery gene
VroAM7_RS14730 (VroAM7_27450)	pilN	3129773..3130360 (-)	588	WP_143693018.1	PilN domain-containing protein	Machinery gene
VroAM7_RS14735 (VroAM7_27460)	pilM	3130344..3131360 (-)	1017	WP_138941723.1	type IV pilus assembly protein PilM	Machinery gene
VroAM7_RS14740 (VroAM7_27470)	-	3131501..3134068 (+)	2568	WP_088880300.1	penicillin-binding protein 1A	-
VroAM7_RS14745 (VroAM7_27480)	oxyR	3134146..3135054 (-)	909	WP_143693019.1	DNA-binding transcriptional regulator OxyR	-
VroAM7_RS14750 (VroAM7_27490)	-	3135229..3135957 (+)	729	WP_005432732.1	redoxin family protein	-

Sequence

Protein

• Length: 338 a.a.
• Molecular weight: 37661.00 Da
• Isoelectric Point: 5.8905

>NTDB_id=74144 VroAM7_RS14735 WP_138941723.1 3130344..3131360(-) (pilM) [Vibrio rotiferianus strain AM7]
MDKLIVTGIDITHRSLKAVILKPTGKSFSLLGYKEIVLNGDIVAENQLINHQEIVNTLKEIKKSLPLFHRKVAIAVPDSA
VISKVLPMDCDLEESEVEFAVAQAFSLQSPIPIDELSLDYVPCDGGSMTKNRQYQVFATRKELVDSRVKLLEKAGLKPAV
VDVHAQVAGEVWQLAAEANPENNSFCLVEVRERDGSITMFNTEGELFHKEFTWSPSEPLVQDGNFSEGGEAEIKATDHFN
QDVAKNIQRQLQLYSSVSGSNDVQGLWLSGSRASTPMLLETLSTCLGIRCEILNIFEHFEIQLSPRRRRDLDWHRFTTAA
GIAVRAIKCLRRGNASTR

Nucleotide

• Length: 1017 bp

>NTDB_id=74144 VroAM7_RS14735 WP_138941723.1 3130344..3131360(-) (pilM) [Vibrio rotiferianus strain AM7]
ATGGATAAGTTAATAGTTACTGGCATTGATATTACTCATCGCAGCCTCAAAGCCGTAATACTAAAACCAACAGGGAAGAG
CTTTTCCCTGCTTGGCTATAAAGAAATTGTACTAAACGGCGACATTGTCGCTGAAAACCAACTCATAAATCATCAAGAAA
TTGTAAATACCCTAAAAGAGATAAAAAAGTCGCTACCACTATTTCATCGAAAAGTAGCGATAGCTGTACCGGACAGTGCT
GTTATCTCAAAGGTTTTGCCGATGGACTGTGACTTAGAAGAGAGCGAAGTTGAGTTTGCAGTGGCACAAGCCTTTTCTCT
TCAATCTCCCATTCCTATAGATGAGCTGAGTTTGGATTATGTCCCTTGTGATGGGGGCAGTATGACTAAGAATAGGCAAT
ATCAGGTCTTTGCGACGAGAAAGGAATTAGTAGATAGCCGAGTTAAGCTGTTGGAAAAAGCAGGATTAAAACCTGCAGTA
GTTGATGTACATGCTCAGGTCGCGGGAGAAGTATGGCAATTAGCGGCTGAGGCCAACCCTGAAAACAACAGTTTTTGCCT
TGTAGAAGTAAGAGAGCGTGATGGTTCGATAACGATGTTTAATACAGAAGGAGAGCTGTTTCATAAAGAGTTTACGTGGA
GCCCTTCTGAGCCATTAGTTCAAGATGGCAACTTCTCTGAAGGAGGAGAGGCTGAAATAAAAGCTACCGATCACTTTAAT
CAAGACGTTGCCAAAAACATTCAAAGGCAGCTTCAGCTTTACTCTTCTGTATCGGGTTCAAATGACGTACAAGGCCTTTG
GTTATCAGGCTCAAGGGCGTCAACACCAATGCTATTAGAAACGCTCTCCACTTGCTTAGGTATTCGTTGTGAGATTCTTA
ATATATTCGAACATTTCGAAATTCAGCTCTCACCAAGGCGCCGTAGGGATTTAGATTGGCACAGGTTTACTACGGCGGCA
GGTATTGCTGTCAGAGCAATAAAGTGCTTGAGGAGGGGGAATGCTTCAACCCGTTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (8-210)

• Type IV pilus assembly protein PilM; (PilM_2) (237-326)

3D structure

• AlphaFold DB: A0A510I8N2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Vibrio campbellii strain DS40M4	54.36	100	0.553
pilM	Vibrio cholerae strain A1552	41.449	100	0.423