Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   N5C08_RS22660 Genome accession   NZ_CP104557
Coordinates   4886844..4888076 (-) Length   410 a.a.
NCBI ID   WP_060479888.1    Uniprot ID   -
Organism   Pseudomonas promysalinigenes strain RL-WG26     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4881844..4893076
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N5C08_RS22645 (N5C08_22645) - 4883500..4885113 (-) 1614 WP_261744320.1 AAA family ATPase -
  N5C08_RS22650 (N5C08_22650) aroB 4885129..4886226 (-) 1098 WP_186476270.1 3-dehydroquinate synthase -
  N5C08_RS22655 (N5C08_22655) aroK 4886321..4886839 (-) 519 WP_060479889.1 shikimate kinase AroK -
  N5C08_RS22660 (N5C08_22660) pilQ 4886844..4888076 (-) 1233 WP_060479888.1 type IV pilus secretin PilQ Machinery gene
  N5C08_RS22665 (N5C08_22665) - 4888036..4889052 (-) 1017 WP_261744322.1 pilus assembly protein PilP -
  N5C08_RS22670 (N5C08_22670) - 4889049..4889585 (-) 537 WP_261744323.1 PilN domain-containing protein -
  N5C08_RS22675 (N5C08_22675) pilM 4889585..4890448 (-) 864 WP_261744324.1 type IV pilus biogenesis protein PilM -

Sequence

Protein


Download         Length: 410 a.a.        Molecular weight: 44406.79 Da        Isoelectric Point: 4.9160

>NTDB_id=730485 N5C08_RS22660 WP_060479888.1 4886844..4888076(-) (pilQ) [Pseudomonas promysalinigenes strain RL-WG26]
MASQACLAAPFRGEAITLNFQDVEVRSVLQVLADYAGINLVASDTVQGNITLRLHDVPWDQALDLVLHSKGLARREQGNV
LLVAPAAELAEQSRGARIKQELDAQLEPLRRELLPIHHAKASDLAELLLATLADDGILTGRGSLSVDTRTNTLVAHQPAE
RLAELRQLVAQLDVPVQQVEIEARIVEANVDYEKGLGVRWGRQLYGDAPLLGKDLFVDLGMDRGAAGIGVGLLRGGVLLD
LELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSKSGATSVAFREASLSLEVTPQITPDGNVIMTVKVTKDE
PDFVNALNDVPPIRKNEVNAKVRVANGETIVIGGVYSTAQNNVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRI
MSDQAIAVSR

Nucleotide


Download         Length: 1233 bp        

>NTDB_id=730485 N5C08_RS22660 WP_060479888.1 4886844..4888076(-) (pilQ) [Pseudomonas promysalinigenes strain RL-WG26]
ATGGCCAGTCAGGCGTGTCTGGCAGCGCCCTTTCGGGGTGAGGCCATAACCCTGAACTTCCAGGATGTGGAAGTGCGATC
AGTGCTCCAGGTGCTGGCTGACTATGCCGGGATCAACTTGGTGGCCAGTGACACCGTGCAAGGCAATATCACCTTGCGGC
TACATGATGTGCCCTGGGATCAAGCGCTGGATTTGGTGTTGCACAGCAAGGGGCTGGCGCGACGTGAGCAGGGCAATGTG
TTGTTGGTCGCGCCCGCCGCAGAGTTGGCCGAGCAGTCTCGCGGTGCCCGTATCAAACAGGAGCTCGACGCACAACTGGA
GCCTTTGCGGCGTGAGCTGCTACCGATTCATCACGCCAAGGCCTCGGACCTTGCCGAGTTGCTGCTGGCGACGCTCGCCG
ATGACGGTATTTTGACCGGCCGTGGCAGCCTGAGTGTCGATACGCGCACCAATACCCTGGTAGCGCACCAGCCGGCTGAG
CGCCTGGCCGAGTTGCGCCAGTTGGTCGCACAGCTTGATGTACCGGTCCAGCAGGTGGAAATCGAGGCGCGTATCGTGGA
GGCCAATGTCGATTACGAAAAGGGCCTGGGGGTGCGCTGGGGGCGGCAGTTGTACGGGGATGCACCACTGTTGGGCAAGG
ACCTGTTCGTCGACCTGGGCATGGACCGGGGCGCAGCTGGCATTGGCGTAGGGTTGTTGCGCGGCGGTGTGCTGCTGGAC
CTGGAGCTCAGCGCGATGGAGAAAAGCGGCAACGGCGAAATCATTTCCCAGCCCAAGGTGGTTACGGCTGACAAGGAAAC
CGCCAGGATTCTCAAAGGCACCGAAGTGCCCTACCAGGAAACCAGCAAAAGCGGGGCGACCTCGGTAGCCTTTCGCGAAG
CCTCGCTGTCACTGGAAGTTACCCCGCAGATCACGCCAGACGGCAATGTCATCATGACGGTCAAAGTGACCAAGGATGAG
CCCGATTTCGTCAATGCCCTCAACGACGTACCCCCGATTCGCAAGAACGAAGTCAATGCCAAGGTTCGGGTAGCGAATGG
CGAAACCATCGTCATCGGTGGCGTTTACTCGACCGCGCAAAACAATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGC
CGTATGTTGGGCGGCTATTTCGACGCGATGCATTACAAGAGAAAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATC
ATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(245-401)

Bacterial type II/III secretion system short domain (Secretin_N)

(113-178)

Secretin and TonB N terminus short domain (STN)

(39-85)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.184

100

0.637
  comQ Acinetobacter baylyi ADP1

43.016

100

0.473
  pilQ Acinetobacter baumannii D1279779

41.485

100

0.463
  pilQ Acinetobacter baumannii strain A118

41.485

100

0.463
  pilQ Legionella pneumophila strain Lp02

44.019

100

0.449
  pilQ Legionella pneumophila strain ERS1305867

44.019

100

0.449
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.41

100

0.429
  pilQ Vibrio cholerae strain A1552

42.41

100

0.429
  pilQ Vibrio campbellii strain DS40M4

41.371

100

0.427
  pilQ Neisseria meningitidis MC58

37.947

100

0.388
  pilQ Neisseria meningitidis M1080

37.947

100

0.388
  pilQ Neisseria gonorrhoeae strain FA1090

37.47

100

0.383