Detailed information    

insolico Bioinformatically predicted

Overview

Name   dprA   Type   Machinery gene
Locus tag   N4269_RS00175 Genome accession   NZ_CP104356
Coordinates   38691..39806 (+) Length   371 a.a.
NCBI ID   WP_000654776.1    Uniprot ID   A0A2K2UIV5
Organism   Vibrio cholerae strain PNUSAV001141     
Function   ssDNA binding; loading RecA onto ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 33691..44806
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N4269_RS00155 (N4269_00155) rsmB 34614..35894 (-) 1281 WP_001102799.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  N4269_RS00160 (N4269_00160) fmt 35950..36897 (-) 948 WP_134986737.1 methionyl-tRNA formyltransferase -
  N4269_RS00165 (N4269_00165) def 36915..37424 (-) 510 WP_000115010.1 peptide deformylase -
  N4269_RS00170 (N4269_00170) - 37564..38691 (+) 1128 WP_175247897.1 LysM peptidoglycan-binding domain-containing protein -
  N4269_RS00175 (N4269_00175) dprA 38691..39806 (+) 1116 WP_000654776.1 DNA-processing protein DprA Machinery gene
  N4269_RS00180 (N4269_00180) - 39809..40285 (+) 477 WP_000979769.1 DUF494 family protein -
  N4269_RS00185 (N4269_00185) - 40299..40865 (+) 567 WP_043990156.1 type I DNA topoisomerase -
  N4269_RS00190 (N4269_00190) - 40938..42071 (-) 1134 WP_134986731.1 5-(carboxyamino)imidazole ribonucleotide synthase -
  N4269_RS00195 (N4269_00195) purE 42075..42560 (-) 486 WP_000215765.1 5-(carboxyamino)imidazole ribonucleotide mutase -
  N4269_RS00200 (N4269_00200) - 42727..43284 (+) 558 WP_175247899.1 L-threonylcarbamoyladenylate synthase -
  N4269_RS00205 (N4269_00205) hemF 43303..44220 (+) 918 WP_000443979.1 oxygen-dependent coproporphyrinogen oxidase -

Sequence

Protein


Download         Length: 371 a.a.        Molecular weight: 39960.95 Da        Isoelectric Point: 7.1952

>NTDB_id=729033 N4269_RS00175 WP_000654776.1 38691..39806(+) (dprA) [Vibrio cholerae strain PNUSAV001141]
MKDQDLAAWLALCFTPKLGSKTISHLLATRLPAQLQSFTPKQWLASGLKPEQLVFLTTQAAKQAELCLQWRSAANNRYIV
TPHCPLYPRLLKEISSSPPILFIEGIWEAVHDPAVAIVGSRNASVDGRQIARQFATELAQSGLVVTSGLALGIDGYAHDG
ALQAQGQTVAVLGSGLAQVYPKQHQGLAERIIAQGALVSEFAPHTPPKADHFPRRNRIISGLSLGVVVVEAAEKSGSLIT
ARYAAEQGREVFVVPGSIFNAASQGSNQLIRQGACLVQSVQQIHQELKNALTWSLSEQVPYQATLFSAVQGDEELPFPEL
LANVGIEATPIDILASRTQIPVQDIMMQLLELELLGHVVAVPGGYIRKGRG

Nucleotide


Download         Length: 1116 bp        

>NTDB_id=729033 N4269_RS00175 WP_000654776.1 38691..39806(+) (dprA) [Vibrio cholerae strain PNUSAV001141]
ATGAAAGATCAGGATTTAGCGGCATGGTTGGCGCTCTGTTTTACTCCTAAACTAGGCAGCAAAACCATTTCTCACCTGCT
TGCGACCCGTTTGCCAGCCCAGTTGCAAAGTTTTACGCCTAAGCAATGGTTGGCCAGCGGGCTTAAGCCCGAACAACTGG
TATTTTTAACCACTCAAGCGGCTAAGCAAGCCGAGCTGTGTTTGCAATGGCGATCAGCAGCCAATAACCGCTATATCGTC
ACTCCTCATTGCCCGCTTTACCCTCGTTTATTGAAAGAGATTAGCTCATCGCCTCCCATCCTGTTTATCGAAGGAATATG
GGAAGCGGTGCATGACCCTGCGGTGGCTATCGTCGGTAGCCGTAATGCCAGTGTTGATGGGCGGCAGATCGCTCGCCAGT
TTGCCACTGAGCTCGCGCAGTCGGGTTTAGTGGTCACCAGTGGTTTAGCGCTTGGTATTGATGGCTATGCGCACGATGGA
GCTTTGCAAGCACAAGGGCAAACCGTGGCAGTATTAGGCTCAGGGCTGGCACAGGTTTACCCCAAACAGCATCAAGGGTT
AGCGGAGCGAATCATCGCCCAAGGGGCCTTGGTTTCTGAGTTTGCCCCTCACACACCTCCTAAAGCGGATCACTTTCCGC
GCCGTAACCGAATTATCAGCGGCTTATCGCTGGGCGTTGTGGTGGTAGAAGCTGCGGAGAAAAGCGGCTCACTCATCACT
GCGCGCTACGCCGCTGAGCAAGGGCGTGAGGTCTTTGTGGTTCCCGGCTCGATTTTTAATGCTGCCAGCCAAGGTAGCAA
TCAATTGATTCGTCAAGGCGCTTGTTTGGTGCAAAGTGTGCAACAAATTCATCAAGAGCTCAAAAATGCGCTGACTTGGT
CACTCTCTGAACAAGTTCCTTATCAAGCAACACTTTTTTCTGCTGTACAGGGCGATGAAGAATTGCCATTTCCCGAGCTG
TTAGCTAACGTAGGAATAGAAGCTACACCTATTGATATTCTCGCAAGCCGGACCCAGATACCGGTGCAAGATATCATGAT
GCAGCTCTTGGAGCTTGAGCTCCTTGGGCATGTGGTTGCAGTACCTGGTGGCTATATTAGAAAGGGGAGAGGCTAG

Domains

Predicted by InterproScan.

DNA recombination-mediator protein A (DNA_processg_A)

(77-278)

DprA winged helix domain (DprA_WH)

(319-364)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K2UIV5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  dprA Vibrio cholerae strain A1552

98.652

100

0.987
  dprA Vibrio campbellii strain DS40M4

58.108

99.73

0.58
  dprA Legionella pneumophila strain ERS1305867

43.583

100

0.439
  dprA Glaesserella parasuis strain SC1401

43.351

100

0.439
  dprA Neisseria meningitidis strain C311

37.594

100

0.404
  dprA Neisseria meningitidis MC58

37.594

100

0.404
  dprA Neisseria gonorrhoeae strain FA1090

36.776

100

0.394
  dprA Acinetobacter baumannii D1279779

43.505

89.218

0.388
  dprA Acinetobacter baumannii strain A118

43.202

89.218

0.385
  dprA Neisseria gonorrhoeae MS11

35.949

100

0.383
  dprA Acinetobacter baylyi ADP1

42.042

89.757

0.377
  dprA Haemophilus influenzae Rd KW20

48.746

75.202

0.367