Detailed information NTG0727627

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: N0U38_RS24790
• Genome accession: NZ_CP104107
• Coordinates: 5439592..5440782 (-)
• Length: 396 a.a.
• NCBI ID: WP_260270587.1
• Uniprot ID: -
• Organism: Pseudomonas mosselii strain PH4
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type	MGE coordinates	Gene coordinates	Relative position	Distance (bp)
Genomic island	5441438..5452713	5439592..5440782	flank	656

Gene organization within MGE regions

Location: 5439592..5452713

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
N0U38_RS24790 (N0U38_24790)	pilQ	5439592..5440782 (-)	1191	WP_260270587.1	type IV pilus secretin PilQ	Machinery gene
N0U38_RS24795 (N0U38_24795)	-	5440754..5441767 (-)	1014	WP_084942060.1	pilus assembly protein PilP	-
N0U38_RS24800 (N0U38_24800)	-	5441764..5442303 (-)	540	WP_062575467.1	PilN domain-containing protein	-
N0U38_RS24805 (N0U38_24805)	pilM	5442300..5443196 (-)	897	WP_260270588.1	type IV pilus biogenesis protein PilM	-
N0U38_RS24810 (N0U38_24810)	-	5443366..5445816 (+)	2451	WP_375547233.1	penicillin-binding protein 1A	-
N0U38_RS24815 (N0U38_24815)	-	5445912..5447180 (-)	1269	WP_028689114.1	malic enzyme-like NAD(P)-binding protein	-
N0U38_RS24820 (N0U38_24820)	-	5447360..5448181 (-)	822	WP_260270589.1	thermonuclease family protein	-
N0U38_RS24825 (N0U38_24825)	rpmE	5448184..5448399 (-)	216	WP_011531746.1	50S ribosomal protein L31	-
N0U38_RS24830 (N0U38_24830)	-	5448569..5450788 (+)	2220	WP_028689116.1	primosomal protein N'	-
N0U38_RS24835 (N0U38_24835)	argS	5450977..5452713 (+)	1737	WP_260270590.1	arginine--tRNA ligase	-

Sequence

Protein

• Length: 396 a.a.
• Molecular weight: 42864.16 Da
• Isoelectric Point: 4.8325

>NTDB_id=727627 N0U38_RS24790 WP_260270587.1 5439592..5440782(-) (pilQ) [Pseudomonas mosselii strain PH4]
MTQAETFQGEPVSLNLQDVELRSVLQVMADHAGVNLVVGDSVQGRITLRLEDVPWEQALDLVLRSKGLGRRQEGNVLLVA
PVAELAGQVQGIEPLQRELIPVFHAKAGELAELLLASLADDGAVAGRGSLSVDARTNTLIVTQPAARLAELRRLVTQLDV
PVRQVMIEARIVEANVDYEKALGVRWGGPLYGSDPRLGSDLFVDLGVERGGAALGVGLLRSDILLDLELSAMEKSGNGEI
ISQPKVVTADKETARILKGTEVPYQETTRSGATSVSFREASLSLEVTPQITPDGKVIMAVRVTKDEPDFINALNDVPPIR
KNEVNAKVRVTDGQTIVIGGVYSTSQNKVVDKVPFFGDLPYVGRLFRRDALQEKKSELLVFLTPRIMSDQAIAVSR

Nucleotide

• Length: 1191 bp

>NTDB_id=727627 N0U38_RS24790 WP_260270587.1 5439592..5440782(-) (pilQ) [Pseudomonas mosselii strain PH4]
ATGACGCAGGCCGAGACGTTTCAGGGTGAGCCGGTGTCGCTCAACCTCCAGGACGTCGAGCTGCGTTCGGTGCTTCAGGT
CATGGCCGATCACGCGGGTGTCAACCTGGTGGTCGGCGACTCGGTGCAGGGGCGGATTACCCTGCGCCTGGAGGATGTGC
CATGGGAGCAGGCGCTTGATCTGGTGCTGCGCAGCAAGGGCCTGGGCAGGCGCCAGGAGGGGAATGTGCTGCTGGTTGCT
CCTGTGGCAGAGCTCGCTGGGCAGGTCCAGGGCATCGAGCCACTGCAGCGTGAATTGATCCCTGTCTTCCATGCCAAGGC
CGGCGAGCTGGCGGAGCTGCTGCTGGCGAGCCTGGCGGACGACGGTGCCGTGGCCGGGCGTGGCAGCCTCAGTGTGGACG
CTCGCACCAATACCTTGATAGTCACCCAGCCTGCCGCGCGGCTTGCCGAGCTGCGCAGGCTGGTCACGCAACTGGATGTG
CCGGTGCGCCAGGTCATGATCGAGGCACGGATCGTCGAGGCCAATGTCGACTACGAAAAGGCACTGGGCGTGCGCTGGGG
TGGACCGCTGTATGGCTCGGACCCCCGGCTGGGCAGCGACCTTTTCGTCGATCTTGGCGTCGAGCGGGGAGGTGCGGCGT
TGGGGGTGGGGCTGCTGCGCAGCGATATCCTGCTCGACCTGGAGCTCAGCGCCATGGAAAAAAGCGGCAATGGCGAGATC
ATCTCCCAGCCCAAGGTGGTTACGGCCGACAAGGAAACCGCGCGCATACTCAAGGGTACCGAAGTGCCGTACCAGGAAAC
CACCCGCAGTGGCGCCACTTCGGTCTCCTTCCGCGAGGCGTCCCTGTCGCTGGAGGTGACGCCGCAGATCACGCCGGACG
GCAAGGTGATCATGGCGGTGCGGGTGACCAAGGACGAGCCGGACTTCATCAATGCCCTCAACGATGTGCCGCCGATCCGC
AAGAACGAGGTCAACGCCAAGGTGCGGGTGACCGATGGGCAGACCATTGTGATTGGCGGCGTGTACTCGACGTCGCAGAA
CAAAGTGGTCGACAAGGTGCCATTTTTTGGTGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGA
AAAAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

• Bacterial type II and III secretion system protein (Secretin) (231-387)

• Bacterial type II/III secretion system short domain (Secretin_N) (99-164)

• Secretin and TonB N terminus short domain (STN) (34-81)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Pseudomonas aeruginosa PAK	57.11	100	0.629
comQ	Acinetobacter baylyi ADP1	42.729	100	0.482
pilQ	Acinetobacter baumannii D1279779	40.302	100	0.472
pilQ	Acinetobacter baumannii strain A118	40.302	100	0.472
pilQ	Legionella pneumophila strain ERS1305867	42.654	100	0.455
pilQ	Legionella pneumophila strain Lp02	42.654	100	0.455
pilQ	Vibrio campbellii strain DS40M4	39.858	100	0.427
pilQ	Vibrio cholerae O1 biovar El Tor strain E7946	39.904	100	0.419
pilQ	Vibrio cholerae strain A1552	39.904	100	0.419
pilQ	Neisseria meningitidis M1080	36.407	100	0.389
pilQ	Neisseria gonorrhoeae strain FA1090	36.256	100	0.386
pilQ	Neisseria meningitidis MC58	35.545	100	0.379