Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA2   Type   Machinery gene
Locus tag   N0B71_RS13205 Genome accession   NZ_CP104011
Coordinates   2878282..2878683 (+) Length   133 a.a.
NCBI ID   WP_259759289.1    Uniprot ID   -
Organism   Pseudomonas sp. GCEP-101 isolate Gut     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2873282..2883683
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B71_RS13180 yacG 2873386..2873589 (-) 204 WP_226046023.1 DNA gyrase inhibitor YacG -
  N0B71_RS13185 coaE 2873586..2874197 (-) 612 WP_259759285.1 dephospho-CoA kinase -
  N0B71_RS13190 pilD 2874259..2875131 (-) 873 WP_259759286.1 A24 family peptidase Machinery gene
  N0B71_RS13195 pilC 2875134..2876351 (-) 1218 WP_259759287.1 type II secretion system F family protein Machinery gene
  N0B71_RS13200 pilB 2876355..2878055 (-) 1701 WP_259759288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N0B71_RS13205 pilA2 2878282..2878683 (+) 402 WP_259759289.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N0B71_RS13215 galE 2878915..2879931 (-) 1017 WP_259759290.1 UDP-glucose 4-epimerase GalE -
  N0B71_RS13220 nadC 2880058..2880906 (-) 849 WP_259759291.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N0B71_RS13225 - 2881186..2883477 (+) 2292 WP_259759292.1 DUF1631 domain-containing protein -

Sequence

Protein


Download         Length: 133 a.a.        Molecular weight: 13458.62 Da        Isoelectric Point: 7.5854

>NTDB_id=726976 N0B71_RS13205 WP_259759289.1 2878282..2878683(+) (pilA2) [Pseudomonas sp. GCEP-101 isolate Gut]
MKAQKGFTLIELMIVVAIIGILAAIALPAYNDYTKKARFSEVMSVGEGYKTPVAQCIQENNGATGCTAGANGIPAIPATL
PANIASMSVTDGVILVTGTAAAGGYTWSVAPVVDGGATRFPQTGTCLAAGYCK

Nucleotide


Download         Length: 402 bp        

>NTDB_id=726976 N0B71_RS13205 WP_259759289.1 2878282..2878683(+) (pilA2) [Pseudomonas sp. GCEP-101 isolate Gut]
ATGAAGGCTCAAAAAGGCTTCACCCTGATCGAACTGATGATCGTGGTTGCCATCATCGGCATCCTGGCCGCCATCGCCCT
GCCGGCCTACAACGATTACACCAAAAAGGCCCGTTTCTCCGAAGTCATGTCGGTTGGCGAAGGCTATAAGACTCCGGTTG
CCCAATGCATTCAGGAAAACAACGGCGCGACTGGCTGCACCGCTGGTGCGAATGGCATTCCTGCTATTCCGGCAACTCTG
CCTGCAAACATCGCTTCCATGTCCGTGACTGATGGTGTGATTCTCGTTACCGGTACTGCCGCCGCTGGTGGCTACACTTG
GAGCGTAGCGCCGGTTGTAGACGGTGGTGCGACTCGCTTCCCGCAGACTGGCACTTGCCTGGCCGCAGGCTACTGCAAGT
AA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA2 Legionella pneumophila str. Paris

49.231

97.744

0.481
  pilA2 Legionella pneumophila strain ERS1305867

48.462

97.744

0.474
  comP Acinetobacter baylyi ADP1

45.588

100

0.466
  pilA Haemophilus influenzae Rd KW20

44.538

89.474

0.398
  pilA/pilA1 Eikenella corrodens VA1

37.681

100

0.391
  pilA Ralstonia pseudosolanacearum GMI1000

43.966

87.218

0.383
  pilA Glaesserella parasuis strain SC1401

44.144

83.459

0.368
  pilA Vibrio parahaemolyticus RIMD 2210633

38.71

93.233

0.361