Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   N0B71_RS13195 Genome accession   NZ_CP104011
Coordinates   2875134..2876351 (-) Length   405 a.a.
NCBI ID   WP_259759287.1    Uniprot ID   -
Organism   Pseudomonas sp. GCEP-101 isolate Gut     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2870134..2881351
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B71_RS13155 - 2870762..2870932 (-) 171 WP_065084249.1 DUF3094 domain-containing protein -
  N0B71_RS13160 - 2871027..2871656 (-) 630 WP_259759284.1 DUF1780 domain-containing protein -
  N0B71_RS13165 - 2871778..2872212 (+) 435 WP_259759552.1 FAD/FMN-containing dehydrogenase -
  N0B71_RS13170 - 2872209..2872898 (+) 690 WP_226046021.1 energy-coupling factor ABC transporter permease -
  N0B71_RS13175 - 2873005..2873220 (+) 216 WP_226046022.1 hypothetical protein -
  N0B71_RS13180 yacG 2873386..2873589 (-) 204 WP_226046023.1 DNA gyrase inhibitor YacG -
  N0B71_RS13185 coaE 2873586..2874197 (-) 612 WP_259759285.1 dephospho-CoA kinase -
  N0B71_RS13190 pilD 2874259..2875131 (-) 873 WP_259759286.1 A24 family peptidase Machinery gene
  N0B71_RS13195 pilC 2875134..2876351 (-) 1218 WP_259759287.1 type II secretion system F family protein Machinery gene
  N0B71_RS13200 pilB 2876355..2878055 (-) 1701 WP_259759288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N0B71_RS13205 pilA2 2878282..2878683 (+) 402 WP_259759289.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N0B71_RS13215 galE 2878915..2879931 (-) 1017 WP_259759290.1 UDP-glucose 4-epimerase GalE -
  N0B71_RS13220 nadC 2880058..2880906 (-) 849 WP_259759291.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 44099.68 Da        Isoelectric Point: 9.9133

>NTDB_id=726974 N0B71_RS13195 WP_259759287.1 2875134..2876351(-) (pilC) [Pseudomonas sp. GCEP-101 isolate Gut]
MADKALKTSVFLWEGTDRKGGKIKGELSGASTALIKAQLRKQGINPTKVRKKGISLFSKGKKIKPLDIALFTRQMATMMG
SGVPLLQSFDIIAEGFENPNMRKLVEEVKQEVAAGNSLANSLRKKPQYFDDLYCNLVDAGEQSGALETLLDRVATYKEKT
ESLKAKIKKAMTYPIAVIIVAIVVSAILLIKVVPQFQSVFEGFGAELPAFTRMVVSLSEVLTAWWFIVLLGLFGVAFGFR
EFHRRSENFRNSVDRGLLKIPVVGDIIYKAAVARYARTLSTTFAAGVPLVEALDSVSGATGNVVFRNAVSRIKQDVSSGT
QLNFSMRTTGVFPSMAVQMAAIGEESGSLDAMLDKVATYYEEEVDNAVDNLTTLMEPMIMAVLGVLVGGLVIAMYLPIFQ
LGKVV

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=726974 N0B71_RS13195 WP_259759287.1 2875134..2876351(-) (pilC) [Pseudomonas sp. GCEP-101 isolate Gut]
ATGGCGGACAAAGCATTAAAGACGAGTGTCTTTCTCTGGGAAGGCACCGACAGAAAGGGCGGGAAGATCAAAGGGGAGTT
GTCAGGAGCCAGTACTGCACTGATCAAGGCGCAACTGCGTAAGCAGGGCATCAACCCGACTAAGGTCCGAAAGAAGGGCA
TATCCCTGTTCAGCAAGGGCAAGAAGATCAAGCCTCTGGACATCGCCCTGTTTACTCGCCAAATGGCGACTATGATGGGC
TCGGGCGTCCCGCTGCTCCAGTCCTTCGATATCATCGCGGAAGGCTTCGAAAACCCGAACATGCGCAAGCTGGTCGAGGA
AGTCAAACAGGAAGTTGCAGCGGGTAATAGCTTGGCGAACTCACTGCGCAAAAAGCCGCAATACTTCGATGACCTGTATT
GCAACCTCGTCGACGCTGGTGAGCAATCAGGTGCATTGGAAACCTTGCTCGACCGGGTTGCGACCTACAAGGAAAAGACC
GAGTCGCTCAAAGCCAAGATTAAGAAGGCAATGACCTACCCTATTGCCGTCATCATCGTCGCAATCGTGGTTTCCGCAAT
CCTTCTAATCAAGGTTGTTCCCCAGTTTCAGTCAGTATTCGAAGGGTTCGGCGCCGAGCTCCCCGCGTTTACCAGAATGG
TCGTTAGCCTTTCCGAAGTGCTCACTGCATGGTGGTTCATCGTGCTGCTAGGACTTTTTGGCGTCGCCTTTGGTTTTCGC
GAGTTCCACCGTCGCTCGGAAAACTTCCGAAACTCTGTCGATCGCGGCCTGCTGAAAATCCCGGTTGTCGGGGACATCAT
TTACAAAGCGGCAGTCGCAAGATATGCACGTACGCTATCGACGACTTTTGCTGCGGGTGTACCACTCGTCGAAGCTCTGG
ACTCGGTTTCCGGAGCAACGGGCAACGTGGTTTTTCGTAATGCCGTCTCCCGTATCAAGCAGGACGTATCTAGCGGGACC
CAATTGAACTTCTCGATGCGCACCACTGGGGTTTTTCCGAGCATGGCAGTGCAAATGGCAGCCATCGGAGAAGAGTCAGG
CTCACTGGATGCCATGCTCGATAAGGTCGCAACATACTACGAAGAAGAAGTGGATAACGCCGTCGATAATCTGACCACCC
TGATGGAGCCGATGATCATGGCGGTCCTAGGTGTACTGGTCGGCGGCTTGGTCATCGCCATGTACCTTCCCATTTTCCAG
CTCGGCAAAGTCGTCTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(276-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

79.506

100

0.795
  pilC Acinetobacter baylyi ADP1

60.099

100

0.602
  pilC Acinetobacter baumannii D1279779

59.901

99.753

0.598
  pilC Legionella pneumophila strain ERS1305867

55.303

97.778

0.541
  pilG Neisseria gonorrhoeae MS11

44.279

99.259

0.44
  pilG Neisseria meningitidis 44/76-A

44.279

99.259

0.44
  pilC Vibrio cholerae strain A1552

40.247

100

0.402
  pilC Vibrio campbellii strain DS40M4

40.657

97.778

0.398
  pilC Thermus thermophilus HB27

37.656

99.012

0.373