Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N0B71_RS13200 Genome accession   NZ_CP104011
Coordinates   2876355..2878055 (-) Length   566 a.a.
NCBI ID   WP_259759288.1    Uniprot ID   -
Organism   Pseudomonas sp. GCEP-101 isolate Gut     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2871355..2883055
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N0B71_RS13165 - 2871778..2872212 (+) 435 WP_259759552.1 FAD/FMN-containing dehydrogenase -
  N0B71_RS13170 - 2872209..2872898 (+) 690 WP_226046021.1 energy-coupling factor ABC transporter permease -
  N0B71_RS13175 - 2873005..2873220 (+) 216 WP_226046022.1 hypothetical protein -
  N0B71_RS13180 yacG 2873386..2873589 (-) 204 WP_226046023.1 DNA gyrase inhibitor YacG -
  N0B71_RS13185 coaE 2873586..2874197 (-) 612 WP_259759285.1 dephospho-CoA kinase -
  N0B71_RS13190 pilD 2874259..2875131 (-) 873 WP_259759286.1 A24 family peptidase Machinery gene
  N0B71_RS13195 pilC 2875134..2876351 (-) 1218 WP_259759287.1 type II secretion system F family protein Machinery gene
  N0B71_RS13200 pilB 2876355..2878055 (-) 1701 WP_259759288.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N0B71_RS13205 pilA2 2878282..2878683 (+) 402 WP_259759289.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  N0B71_RS13215 galE 2878915..2879931 (-) 1017 WP_259759290.1 UDP-glucose 4-epimerase GalE -
  N0B71_RS13220 nadC 2880058..2880906 (-) 849 WP_259759291.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62550.57 Da        Isoelectric Point: 5.9229

>NTDB_id=726975 N0B71_RS13200 WP_259759288.1 2876355..2878055(-) (pilB) [Pseudomonas sp. GCEP-101 isolate Gut]
MNDQVILSGLARQLVLSQLLDEKSAQQAQQQAQRNKLSLVTYLVQNKLVKSRPLTELAAEQFGVAFCDLGSLDRDAFPKE
LISEKLVRQHRVIPLWRRGNKLFVGVSDPTNHQAITDVQFSTGLTTEAILVEDDKLGEAIEKLFESANDALSELGDVDLD
GLDIESASEQKDEAASNDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTMPTLWGEKIVMRILDSASAQMGIDALGYEDAQKQLYLDALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVAAFNLATSVNLIIAQRLARKLCPHCKKEHDVPREALLHEGFPA
ERIGSFKLYSPVGCENCKGGYKGRVGIYEVVKNTPSLQRIIMEEGNSIQIADQARKEGFNDLRTSGLMKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=726975 N0B71_RS13200 WP_259759288.1 2876355..2878055(-) (pilB) [Pseudomonas sp. GCEP-101 isolate Gut]
ATGAACGATCAAGTCATCCTGTCCGGGCTTGCCCGCCAATTGGTGCTTTCGCAGTTGCTTGATGAGAAAAGCGCTCAGCA
AGCCCAACAGCAGGCCCAGCGCAACAAGCTTTCGCTGGTGACCTATCTGGTGCAGAACAAGCTGGTCAAAAGCAGGCCTC
TGACTGAGCTGGCGGCCGAGCAGTTCGGCGTAGCTTTCTGTGACTTGGGTAGCCTGGATCGCGATGCTTTTCCCAAGGAG
CTAATCAGCGAAAAGCTGGTTCGCCAGCATCGGGTAATTCCCCTATGGCGGCGTGGAAACAAGCTGTTCGTGGGAGTTTC
CGATCCAACCAATCACCAGGCCATCACCGACGTCCAATTCAGCACAGGGCTGACCACGGAAGCCATTCTGGTCGAGGACG
ATAAGCTCGGCGAAGCCATCGAGAAGTTGTTCGAAAGCGCCAACGATGCGCTGAGTGAATTGGGCGATGTCGACCTTGAT
GGTCTCGACATCGAAAGCGCCTCCGAACAAAAGGATGAAGCAGCTTCTAACGACGCCGATGACGCTCCCGTCGTTCGCTT
CGTCAATAAGATGCTGCTGGACGCTATCAAGGGCGGATCCTCGGACTTGCACTTCGAACCATATGAGAAAGTCTACCGAG
TGCGTTTCCGCACCGACGGCATGCTCCATGAGGTGGCCAAACCACCTATCCAGTTGGCCAGCCGTATTTCTGCGCGCCTG
AAGGTCATGGCGGGCCTGGATATTTCCGAGCGCCGCAAACCTCAGGACGGCCGCATTAAAATGCGTGTGTCGAAAACCAA
ATCTATCGATTTCCGCGTGAACACCATGCCCACCCTGTGGGGTGAAAAGATTGTCATGCGAATCCTCGATTCCGCCAGCG
CGCAGATGGGAATTGACGCGTTGGGGTACGAGGACGCGCAGAAGCAGCTGTACCTCGATGCCCTTAAGCAACCTCAGGGC
ATGATTTTGGTGACCGGCCCCACGGGGTCAGGCAAGACAGTATCCCTGTATACAGGTCTGAACATCCTCAACACCCCCGA
TATCAATATCTCCACAGCCGAAGATCCGGTAGAGATCAATCTCGAGGGCATTAACCAAGTCAATGTAAACCCTCGTCAGG
GAATGGACTTCTCTCAGGCGCTTCGCGCGTTTCTCCGCCAGGACCCCGATGTGATCATGGTCGGGGAAATCCGCGACTTG
GAAACCGCGGAAATTGCCATCAAGGCGGCGCAAACCGGACACATGGTGATGTCGACGCTGCACACCAACAGCGCAGCGGA
AACTCTTACTCGTCTGCTCAATATGGGTGTAGCCGCGTTCAACCTCGCAACCTCGGTCAACCTGATCATCGCTCAACGAC
TTGCCCGTAAACTTTGCCCCCACTGCAAGAAGGAGCACGATGTTCCCCGAGAAGCATTGCTGCATGAGGGCTTCCCGGCC
GAGCGGATTGGCTCCTTCAAGCTTTACTCCCCGGTCGGATGCGAAAATTGCAAGGGCGGTTACAAAGGACGTGTGGGCAT
TTATGAAGTAGTTAAAAACACGCCCAGCCTGCAGCGCATTATCATGGAGGAAGGCAACTCCATTCAGATCGCCGATCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACTTCGGGACTGATGAAGGCAATGCAAGGCATCACCAGTCTCGAAGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(190-457)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.117

99.293

0.567
  pilB Acinetobacter baumannii D1279779

56.738

99.647

0.565
  pilB Legionella pneumophila strain ERS1305867

55.221

99.823

0.551
  pilB Vibrio cholerae strain A1552

50.263

100

0.507
  pilF Neisseria gonorrhoeae MS11

50

99.293

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

50.741

95.406

0.484
  pilB Vibrio campbellii strain DS40M4

50.835

95.23

0.484
  pilF Thermus thermophilus HB27

38.448

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.454

93.463

0.378
  pilB/pilB1 Synechocystis sp. PCC 6803

39.306

91.696

0.36