Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NP165_RS01085 Genome accession   NZ_CP102096
Coordinates   224887..226542 (+) Length   551 a.a.
NCBI ID   WP_371133701.1    Uniprot ID   -
Organism   Vibrio japonicus strain JCM 31412     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 219887..231542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP165_RS01065 (NP165_01065) pilM 222141..223136 (+) 996 WP_257084528.1 type IV pilus assembly protein PilM Machinery gene
  NP165_RS01070 (NP165_01070) pilN 223126..223707 (+) 582 WP_257084529.1 PilN domain-containing protein Machinery gene
  NP165_RS01075 (NP165_01075) pilO 223700..224290 (+) 591 WP_257084530.1 type 4a pilus biogenesis protein PilO Machinery gene
  NP165_RS01080 (NP165_01080) pilP 224280..224795 (+) 516 WP_257084531.1 pilus assembly protein PilP Machinery gene
  NP165_RS01085 (NP165_01085) pilQ 224887..226542 (+) 1656 WP_371133701.1 type IV pilus secretin PilQ Machinery gene
  NP165_RS01090 (NP165_01090) aroK 226720..227238 (+) 519 WP_257084533.1 shikimate kinase AroK -
  NP165_RS01095 (NP165_01095) aroB 227269..228372 (+) 1104 WP_257084534.1 3-dehydroquinate synthase -
  NP165_RS01100 (NP165_01100) - 228466..229986 (+) 1521 WP_257084535.1 SPOR domain-containing protein -
  NP165_RS01105 (NP165_01105) - 230054..230881 (+) 828 WP_257084536.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 551 a.a.        Molecular weight: 60121.29 Da        Isoelectric Point: 4.3666

>NTDB_id=714336 NP165_RS01085 WP_371133701.1 224887..226542(+) (pilQ) [Vibrio japonicus strain JCM 31412]
MLFTTAAAAQEDVSNKLENIDFRVDGEQNAQIVVQLANPRVAVDVQKAQEGLSIELLNTDVADEKVYLLDVKDFSTNVES
IEVFRKEPSTLLVAAIEGEYNYDYTLNGNYLEVIVKKVSKDEAPKQKSILEGEGKKISINFQDIPVRNVLQLIADYNDFN
LVVSDSVSGNLTLRLDGVPWQQVLDIILKVKGLDKRVDGNVVLVAPKAELDLREKQSLEQARMEEELGDLYSEIIKVNFA
KASEIAELIGGDGTVSMLSERGSLSVDERTNALLIRDLRENIDVIRDIIETLDVAVKQVQIEARIVTVSEGNLDELGVRW
GFTSTNGNNTVGGSIESNLFQAGLYEGSGDGLPIDEFLNVNLAAASSNASSIAFQVAKLGSDLLLDLELSALQRESKAEV
ISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTIAFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEVVKTGHGEAVA
IKTQRIGTQVLVNNGETVVLGGIFQHNVTDTVDKVPLLGDIPLLGALFRRSYQQMGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1656 bp        

>NTDB_id=714336 NP165_RS01085 WP_371133701.1 224887..226542(+) (pilQ) [Vibrio japonicus strain JCM 31412]
ATGTTATTTACGACCGCCGCTGCGGCGCAGGAAGACGTAAGTAACAAATTGGAAAATATAGACTTTAGAGTTGATGGGGA
GCAAAACGCACAAATTGTTGTTCAGTTAGCCAATCCAAGAGTGGCCGTGGATGTGCAAAAAGCTCAAGAAGGGTTGAGTA
TTGAGTTACTTAATACCGATGTAGCAGATGAAAAAGTCTATCTCCTCGATGTAAAAGACTTCTCTACAAATGTGGAATCA
ATCGAAGTCTTTCGAAAGGAACCAAGTACGCTTTTGGTTGCCGCTATCGAAGGTGAATATAACTATGACTACACATTAAA
CGGCAACTATCTCGAAGTTATCGTTAAAAAAGTCTCTAAAGATGAGGCGCCAAAGCAGAAAAGTATTCTAGAAGGGGAAG
GGAAAAAAATCTCGATCAACTTCCAAGACATCCCTGTGCGTAATGTGTTGCAATTAATCGCTGATTACAACGATTTTAAC
TTAGTGGTTTCAGACTCTGTCTCGGGCAATTTAACCTTACGACTGGATGGCGTACCTTGGCAGCAAGTACTCGACATTAT
CCTTAAAGTGAAAGGTTTGGATAAGCGTGTTGATGGCAATGTTGTACTCGTTGCCCCAAAAGCGGAACTTGATCTCAGAG
AAAAGCAATCACTTGAACAAGCACGCATGGAAGAAGAGTTAGGGGACCTCTACTCGGAAATCATTAAAGTGAATTTTGCT
AAAGCGTCTGAAATTGCGGAACTGATCGGAGGCGATGGCACAGTGAGTATGTTGTCCGAGCGTGGTTCATTGAGCGTGGA
TGAACGTACTAACGCTTTGTTAATCCGTGATTTGAGGGAAAACATTGATGTTATTCGTGACATCATTGAGACGCTGGATG
TGGCCGTAAAACAGGTTCAAATTGAAGCGCGAATCGTCACGGTCAGTGAAGGTAACTTAGATGAGCTTGGTGTTCGCTGG
GGTTTTACCTCGACCAACGGTAACAATACAGTCGGTGGTTCGATTGAAAGCAATTTATTCCAAGCTGGATTATACGAAGG
TAGCGGTGATGGCTTGCCAATCGATGAATTCCTCAATGTGAATTTAGCTGCTGCTTCTTCAAATGCGTCATCGATTGCCT
TTCAGGTTGCTAAACTAGGTTCAGACTTACTGCTGGATTTAGAGTTATCTGCACTTCAGCGTGAATCGAAAGCGGAAGTG
ATTTCTAGCCCACGTTTGATTACGACGAACAAGAAACCGGCTTATATTGAGCAGGGTACAGAAATTCCTTATCTGGAATC
TTCTTCAAGTGGTGCGACAACGATCGCCTTTAAGAAAGCCGTCCTTAGTTTAAAAGTGACACCGCAAATCACGCCAGATA
ACCGTTTAGTGCTTGATTTAAGTGTGACGCAAGACAGACCGGGCGAAGTGGTGAAAACAGGTCATGGTGAAGCGGTAGCG
ATCAAAACTCAGCGTATCGGTACGCAAGTTCTGGTGAACAATGGTGAAACTGTCGTCTTGGGAGGTATATTCCAACACAA
TGTGACCGACACCGTTGATAAGGTGCCTTTGTTGGGTGATATCCCTCTTTTAGGGGCATTATTCCGTCGAAGTTATCAAC
AAATGGGTAAGAGTGAACTGCTAATTTTTGTTACGCCAAAGGTAGTGATTCAGTAG

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(159-207)

Bacterial type II/III secretion system short domain (Secretin_N)

(233-298)

Bacterial type II and III secretion system protein (Secretin)

(391-549)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae strain A1552

78.04

100

0.78
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

78.04

100

0.78
  pilQ Vibrio campbellii strain DS40M4

75.636

99.819

0.755
  comQ Acinetobacter baylyi ADP1

38.957

100

0.407
  pilQ Legionella pneumophila strain Lp02

40.768

99.274

0.405
  pilQ Legionella pneumophila strain ERS1305867

40.585

99.274

0.403
  pilQ Acinetobacter baumannii D1279779

37.889

100

0.397
  pilQ Acinetobacter baumannii strain A118

37.889

100

0.397
  pilQ Pseudomonas aeruginosa PAK

39.564

100

0.396