Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   NP165_RS01065 Genome accession   NZ_CP102096
Coordinates   222141..223136 (+) Length   331 a.a.
NCBI ID   WP_257084528.1    Uniprot ID   -
Organism   Vibrio japonicus strain JCM 31412     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 217141..228136
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP165_RS01050 (NP165_01050) - 217637..218365 (-) 729 WP_257084525.1 glutathione peroxidase -
  NP165_RS01055 (NP165_01055) oxyR 218535..219425 (+) 891 WP_257084526.1 DNA-binding transcriptional regulator OxyR -
  NP165_RS01060 (NP165_01060) - 219491..222001 (-) 2511 WP_257084527.1 penicillin-binding protein 1A -
  NP165_RS01065 (NP165_01065) pilM 222141..223136 (+) 996 WP_257084528.1 type IV pilus assembly protein PilM Machinery gene
  NP165_RS01070 (NP165_01070) pilN 223126..223707 (+) 582 WP_257084529.1 PilN domain-containing protein Machinery gene
  NP165_RS01075 (NP165_01075) pilO 223700..224290 (+) 591 WP_257084530.1 type 4a pilus biogenesis protein PilO Machinery gene
  NP165_RS01080 (NP165_01080) pilP 224280..224795 (+) 516 WP_257084531.1 pilus assembly protein PilP Machinery gene
  NP165_RS01085 (NP165_01085) pilQ 224887..226542 (+) 1656 WP_371133701.1 type IV pilus secretin PilQ Machinery gene
  NP165_RS01090 (NP165_01090) aroK 226720..227238 (+) 519 WP_257084533.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 331 a.a.        Molecular weight: 36461.95 Da        Isoelectric Point: 7.0656

>NTDB_id=714332 NP165_RS01065 WP_257084528.1 222141..223136(+) (pilM) [Vibrio japonicus strain JCM 31412]
MGKSQVIGVDIGHHSIKAVALKLSKGTFNLVGYQEIRIESDIISDNHVLNYQKIVKKLKELRKSLPLFSQKVALAIPDNA
VISKVLQIDSGLEGQEREFAIAQAFSHQSPFPVEELSLDFVELGKSGMNQSMEGYQVYATKLDVVESRVQAVSKAGFKPV
LLDVQAHSLVHVWQLASILYDRPDWLLLDVGSTQSSVCMDFQNKAPFCKEIALGTHLIDSSSEMESRVHGDTERFIHALI
EKLQRNIQLLSSVNGADVKGIWLSGGGASTPMLAEEISRRLNLQCELLNPLTLFGTLKLKKSANLSSSHAFTTATGLALR
GLDWMERSHVA

Nucleotide


Download         Length: 996 bp        

>NTDB_id=714332 NP165_RS01065 WP_257084528.1 222141..223136(+) (pilM) [Vibrio japonicus strain JCM 31412]
ATGGGTAAATCTCAAGTCATAGGCGTTGATATTGGTCACCACAGTATTAAAGCTGTCGCTCTCAAACTATCAAAGGGCAC
ATTTAATCTTGTTGGTTACCAAGAGATCAGAATTGAATCAGACATTATCTCTGACAACCATGTGTTAAATTATCAGAAAA
TTGTAAAGAAACTCAAAGAACTAAGAAAGTCTTTGCCGCTATTTAGTCAAAAAGTTGCATTAGCGATTCCCGACAATGCG
GTAATAAGCAAAGTATTACAAATAGATAGCGGACTAGAAGGTCAGGAACGGGAGTTCGCCATCGCTCAAGCATTTTCACA
CCAATCTCCTTTTCCGGTAGAAGAATTGAGTCTCGATTTCGTTGAGCTAGGTAAATCTGGCATGAATCAGTCTATGGAAG
GGTATCAGGTTTATGCAACCAAACTCGATGTTGTTGAGAGTCGAGTACAAGCCGTTAGTAAAGCGGGGTTTAAACCAGTA
TTGCTCGATGTACAGGCACACAGTTTAGTTCATGTTTGGCAGCTCGCTTCTATCTTGTATGACCGACCTGACTGGTTACT
GCTGGATGTTGGCTCAACCCAGTCGTCCGTCTGTATGGACTTTCAAAATAAAGCGCCTTTTTGTAAAGAGATAGCGTTAG
GCACTCATCTTATTGATTCTTCATCGGAAATGGAGAGCAGAGTGCATGGCGATACTGAACGCTTTATTCACGCTTTAATT
GAAAAGCTTCAAAGAAACATTCAATTACTTTCATCGGTGAATGGTGCTGATGTTAAAGGCATTTGGCTGTCCGGAGGGGG
AGCATCAACCCCAATGTTAGCGGAAGAGATCAGTCGTCGTTTAAATCTCCAATGTGAATTATTGAATCCACTTACTCTTT
TTGGAACCCTCAAATTAAAGAAAAGCGCCAACCTCTCTTCGAGTCATGCTTTTACGACGGCGACAGGGCTAGCATTACGA
GGATTAGATTGGATGGAGCGTTCCCATGTTGCATAG

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-213)

Type IV pilus assembly protein PilM; (PilM_2)

(235-321)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio cholerae strain A1552

57.396

100

0.586
  pilM Vibrio campbellii strain DS40M4

47.273

99.698

0.471