Detailed information NTG0001072

Experimentally validated

Overview

• Name: priA
• Type: Machinery gene
• Locus tag: ABD1_RS01805
• Genome accession: NC_020547
• Coordinates: 395463..397691 (-)
• Length: 742 a.a.
• NCBI ID: WP_000156662.1
• Uniprot ID: -
• Organism: Acinetobacter baumannii D1279779
• Function: DNA puliing through the inner membrane DNA binding and uptake

Function

We examined the 33 knockout mutants by comparing their transformation efficiency with wild-type W068. No knockout strains were affected in growth, but 28 of these mutants were severely or completely impaired in natural transformability. These mutations were in 18 genes related to T4P (pilF, pilQ, tsaP, pilM, pilN, pilO, pilP, pilB, pilC, pilT, pilD, pilE, pilY2, pilY1, pilX, pilW, pilV, and fimU), six genes related to DNA uptake and processing (comEA, comA, comF, priA, dprA, and recA), two genes related to T2SS (xcpV and xcpW), and the crp and tonB2 genes.

Genomic Context

Location: 390463..402691

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABD1_RS01785 (ABD1_03360)	-	390647..391603 (-)	957	WP_001107924.1	DnaJ C-terminal domain-containing protein	-
ABD1_RS01790 (ABD1_03370)	-	391754..392365 (-)	612	WP_001105725.1	LysE family translocator	-
ABD1_RS01795 (ABD1_03380)	-	392504..393379 (-)	876	WP_001281765.1	Hsp33 family molecular chaperone HslO	-
ABD1_RS01800 (ABD1_03390)	-	393545..395386 (-)	1842	WP_000008979.1	monovalent cation:proton antiporter-2 (CPA2) family protein	-
ABD1_RS01805 (ABD1_03400)	priA	395463..397691 (-)	2229	WP_000156662.1	primosomal protein N'	Machinery gene
ABD1_RS01810 (ABD1_03410)	gspF	397840..399045 (+)	1206	WP_001112093.1	type II secretion system inner membrane protein GspF	-
ABD1_RS01815 (ABD1_03420)	gspG	399069..399584 (+)	516	WP_000784786.1	type II secretion system major pseudopilin GspG	-
ABD1_RS01820 (ABD1_03430)	-	399852..400196 (+)	345	WP_000643174.1	hypothetical protein	-
ABD1_RS01825 (ABD1_03440)	-	400341..400679 (+)	339	WP_000434938.1	tRNA-binding protein	-
ABD1_RS01830 (ABD1_03450)	lnt	400681..402240 (-)	1560	WP_001203096.1	apolipoprotein N-acyltransferase	-

Sequence

Protein

• Length: 742 a.a.
• Molecular weight: 83803.52 Da
• Isoelectric Point: 8.5630

>NTDB_id=1072 ABD1_RS01805 WP_000156662.1 395463..397691(-) (priA) [Acinetobacter baumannii D1279779]
MTITPKPDSVVYRVRVAVPVHLYDTFDYTLTQAQYERAHVGSRVAISFGRQNLIGIITEKVDPSESFTGTFQLKAISDLL
DEQPILDEQVLSLLTWSAQYYQFPIGEVMQTALPALLRQGKPMDVLFHLWKITPCDNVEALLKRSGKQQDAYQILKLHPA
GTTENILNLSGVETATLKALQKKGLVDCTLEPHDFSPSPVQLAQMPLTLNEDQKKATQYVVNAQHQYQAFLLDGLTGSGK
TEVYLHIMHEVLKQGKQVLVLVPEIGLTPQTISRFKSRFNCDIALLHSGLNDSKRLQAWQQAQTGKASIILGTRSAIYTP
LPRLGLIILDEEHDLSYKQQEGFRYHARDVALYRGHLQGCPVLLGSATPSIDSYHLVETGKLTALQLNQRAGHALLPKMH
LIDLKIVKKQHGISQPLIEQIKNTLARKEQVLIFLNRRGYAPVLVCESCGWQANCPHCDAHFTLHTQPYSYLHCHHCGTV
HRLPDHCPECQQKSLKTLGAGTAKVEEHLQELFPDHDVIRVDRDSTSRVGSWQKIYDRIQQNKPSILLGTQMLAKGHHFP
HVTLVAILDIDAGLLSVDIRAPERTAQLIVQVAGRAGRGEHKGHVYLQTLRPDHPLLTTLIEKDYRAVAKQTLAERKVAL
LPPYRYAVLIRAESKDRDYTLHFLNEAAEQLRQIAGDIVDIWGPIPAPMERKAGRYRAHMVILSADRARLHFYLRQWWAQ
LVHAPRQHQLRLSIDVDPQEFS

Nucleotide

• Length: 2229 bp

>NTDB_id=1072 ABD1_RS01805 WP_000156662.1 395463..397691(-) (priA) [Acinetobacter baumannii D1279779]
ATGACCATAACGCCAAAGCCTGATTCAGTTGTTTATCGTGTGCGTGTAGCCGTACCTGTACATCTGTACGACACTTTTGA
CTATACACTCACCCAAGCACAATATGAACGGGCTCATGTAGGCTCACGCGTCGCTATTTCATTTGGACGCCAAAATCTGA
TTGGCATCATTACTGAAAAAGTTGACCCAAGTGAATCATTTACAGGCACCTTTCAGCTTAAAGCCATTTCTGACCTTTTA
GACGAACAGCCTATTTTAGATGAACAAGTTTTAAGTTTATTGACATGGTCTGCCCAGTATTACCAATTTCCAATTGGCGA
GGTCATGCAAACCGCCCTACCCGCATTATTGCGTCAGGGCAAACCAATGGATGTTTTGTTCCATCTTTGGAAAATCACAC
CATGTGATAATGTTGAAGCACTTCTTAAACGTTCTGGTAAGCAACAAGACGCGTATCAAATTTTAAAATTACACCCTGCC
GGAACTACAGAGAATATTCTTAATTTAAGTGGTGTAGAAACAGCTACGCTTAAAGCACTTCAAAAGAAAGGACTAGTTGA
CTGTACGCTTGAACCACACGACTTTAGCCCATCGCCTGTTCAATTGGCACAAATGCCGCTCACGCTAAATGAAGACCAAA
AGAAAGCAACTCAGTACGTCGTAAACGCACAGCATCAATACCAAGCATTTTTATTAGATGGTTTAACAGGAAGTGGTAAA
ACTGAAGTTTATTTGCACATCATGCATGAAGTACTAAAGCAAGGTAAGCAAGTACTGGTATTAGTTCCTGAAATTGGTTT
AACCCCTCAAACCATTTCGCGCTTTAAGTCTCGTTTTAACTGCGACATTGCTTTATTACATTCGGGGCTAAATGACTCTA
AACGCCTACAGGCATGGCAACAAGCACAGACTGGAAAGGCCTCTATTATTTTAGGAACACGTTCAGCCATTTATACCCCG
CTGCCACGTTTGGGCCTGATCATTTTAGATGAAGAACACGACCTGTCTTATAAACAACAAGAAGGTTTCCGTTACCATGC
TCGTGATGTCGCGCTTTATCGAGGACATTTACAAGGCTGTCCTGTTCTTTTAGGTTCGGCAACCCCAAGCATTGACAGTT
ATCACTTAGTCGAAACTGGCAAGTTAACTGCGCTTCAGCTGAACCAACGTGCAGGCCATGCGCTTTTACCAAAAATGCAT
TTGATTGATCTTAAAATTGTCAAAAAACAGCATGGTATTAGTCAGCCTTTAATCGAACAGATTAAAAATACCTTGGCAAG
AAAAGAACAGGTTTTAATTTTTTTAAACCGACGTGGTTATGCACCTGTTCTGGTCTGTGAAAGTTGTGGTTGGCAAGCGA
ATTGTCCGCATTGTGATGCGCATTTTACGTTGCACACTCAGCCTTATTCTTACTTACATTGCCACCACTGTGGCACCGTT
CATCGCTTGCCAGACCACTGCCCTGAGTGTCAGCAAAAAAGCCTCAAAACTTTAGGTGCTGGCACAGCCAAGGTTGAAGA
GCATTTACAAGAATTATTCCCAGATCATGATGTGATTCGGGTCGATCGTGACAGCACCAGCCGAGTCGGTAGCTGGCAAA
AAATTTATGATCGCATTCAACAAAATAAACCTTCGATTTTGCTCGGTACTCAAATGTTGGCAAAGGGTCACCATTTTCCA
CATGTGACCTTGGTTGCTATTTTAGATATTGATGCAGGTTTACTCAGTGTCGATATTCGAGCGCCAGAGCGTACTGCTCA
ATTGATTGTACAAGTGGCTGGTCGTGCAGGCCGCGGAGAACATAAGGGTCATGTTTATTTACAGACTTTAAGGCCAGATC
ATCCTTTACTCACCACACTCATCGAAAAAGATTATCGGGCAGTCGCGAAACAAACATTGGCTGAGCGTAAAGTTGCATTA
CTGCCGCCTTATCGCTATGCCGTACTTATACGCGCTGAATCGAAAGATCGTGACTATACCTTGCATTTTTTAAATGAAGC
TGCCGAGCAACTTCGCCAAATTGCAGGTGACATTGTTGATATCTGGGGGCCAATTCCAGCGCCAATGGAACGTAAAGCGG
GACGTTACCGCGCACATATGGTGATTTTGTCTGCTGACCGCGCTCGTCTGCATTTTTACTTAAGACAATGGTGGGCTCAA
TTGGTTCATGCGCCAAGACAGCATCAACTCAGACTCTCAATTGATGTTGACCCGCAAGAATTTAGTTAG

Domains

Predicted by InterproScan.

• Primosomal protein N C-terminal domain (PriA_C) (643-738)

• Type III restriction enzyme, res subunit (ResIII) (208-369)

• 3DNA-binding domain (3BD) (PriA_3primeBD) (15-114)

References

• [1]: Yuan Hu et al. (2021) Natural Transformation in Acinetobacter baumannii W068: A Genetic Analysis Reveals the Involvements of the CRP, XcpV, XcpW, TsaP, and TonB₂. Frontiers in Microbiology 12:738034. [PMID: 35126321]