Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   NP165_RS01075 Genome accession   NZ_CP102096
Coordinates   223700..224290 (+) Length   196 a.a.
NCBI ID   WP_257084530.1    Uniprot ID   -
Organism   Vibrio japonicus strain JCM 31412     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 218700..229290
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NP165_RS01060 (NP165_01060) - 219491..222001 (-) 2511 WP_257084527.1 penicillin-binding protein 1A -
  NP165_RS01065 (NP165_01065) pilM 222141..223136 (+) 996 WP_257084528.1 type IV pilus assembly protein PilM Machinery gene
  NP165_RS01070 (NP165_01070) pilN 223126..223707 (+) 582 WP_257084529.1 PilN domain-containing protein Machinery gene
  NP165_RS01075 (NP165_01075) pilO 223700..224290 (+) 591 WP_257084530.1 type 4a pilus biogenesis protein PilO Machinery gene
  NP165_RS01080 (NP165_01080) pilP 224280..224795 (+) 516 WP_257084531.1 pilus assembly protein PilP Machinery gene
  NP165_RS01085 (NP165_01085) pilQ 224887..226542 (+) 1656 WP_371133701.1 type IV pilus secretin PilQ Machinery gene
  NP165_RS01090 (NP165_01090) aroK 226720..227238 (+) 519 WP_257084533.1 shikimate kinase AroK -
  NP165_RS01095 (NP165_01095) aroB 227269..228372 (+) 1104 WP_257084534.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 196 a.a.        Molecular weight: 22899.28 Da        Isoelectric Point: 4.6401

>NTDB_id=714334 NP165_RS01075 WP_257084530.1 223700..224290(+) (pilO) [Vibrio japonicus strain JCM 31412]
MAKYSELDIEEMAEWPILPQLGLILLIGLLLQGVGYWFYLKPKVENLDTLVQKEQELKTTVMIKANKVATLPKLKAQLDE
LSERYDYLSRQLPVQKELASMLASVNEVGLKNSLTFTRIDWGERQNQEFLYRLPLNIELTGDYHNIGDFTQAIAELPRII
NFDDIDWQRVSQESSTLHFRVRAYTYQFKPEVEDEK

Nucleotide


Download         Length: 591 bp        

>NTDB_id=714334 NP165_RS01075 WP_257084530.1 223700..224290(+) (pilO) [Vibrio japonicus strain JCM 31412]
GTGGCTAAATATAGTGAACTCGATATTGAAGAAATGGCTGAATGGCCCATTCTTCCCCAGCTTGGGCTGATTTTGCTCAT
CGGCTTGCTTCTTCAGGGAGTGGGCTATTGGTTTTATTTAAAACCGAAAGTCGAAAACCTCGATACTTTGGTTCAAAAAG
AGCAGGAATTAAAAACGACGGTGATGATTAAAGCGAATAAAGTCGCGACATTGCCGAAGCTAAAAGCGCAGTTAGATGAG
CTTTCAGAGCGCTATGACTATTTGTCGCGACAGCTCCCTGTTCAAAAAGAGCTGGCAAGTATGCTGGCGTCGGTGAATGA
AGTGGGTTTGAAGAACTCTTTGACCTTTACCCGTATCGATTGGGGTGAAAGACAGAATCAAGAATTTCTCTATCGACTGC
CTCTGAACATCGAACTCACAGGTGATTACCACAACATAGGTGATTTCACTCAAGCGATTGCCGAACTGCCGAGAATCATC
AATTTTGATGATATTGATTGGCAGAGAGTCAGCCAAGAAAGTAGCACTCTCCATTTCCGTGTGCGCGCTTATACCTATCA
ATTTAAGCCGGAGGTCGAAGATGAAAAGTAA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(47-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

75

100

0.75
  pilO Vibrio campbellii strain DS40M4

66.316

96.939

0.643