Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   EL386_RS03690 Genome accession   NZ_AP018725
Coordinates   713427..713912 (-) Length   161 a.a.
NCBI ID   WP_126453548.1    Uniprot ID   -
Organism   Sulfuriflexus mobilis strain aks1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 708427..718912
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL386_RS03675 - 709295..710170 (-) 876 WP_126453544.1 prepilin peptidase -
  EL386_RS03680 pilC 710177..711406 (-) 1230 WP_126453546.1 type II secretion system F family protein Machinery gene
  EL386_RS03685 pilB 711410..713125 (-) 1716 WP_172597607.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL386_RS03690 pilE 713427..713912 (-) 486 WP_126453548.1 pilin Machinery gene
  EL386_RS15925 - 714285..714338 (-) 54 WP_420856730.1 hypothetical protein -
  EL386_RS03700 - 714555..715322 (+) 768 WP_126453550.1 hypothetical protein -
  EL386_RS03705 pilR 715413..716798 (-) 1386 WP_126453552.1 sigma-54-dependent transcriptional regulator Regulator
  EL386_RS03710 - 716807..718432 (-) 1626 WP_126453554.1 sensor histidine kinase -
  EL386_RS03715 - 718464..718697 (-) 234 WP_126453556.1 PP0621 family protein -

Sequence

Protein


Download         Length: 161 a.a.        Molecular weight: 16425.98 Da        Isoelectric Point: 8.4919

>NTDB_id=70539 EL386_RS03690 WP_126453548.1 713427..713912(-) (pilE) [Sulfuriflexus mobilis strain aks1]
MKTIQKGFTLIELMIVVAIIGILAAVALPAYQDYTVRAKVSEVILAASSCRTAITEAVQSAANVNMGTTLQNVCSFTPTK
YVASGLVTSAGLIEVTGNATNLGGSTSATANTIRMSPLVTSTARALNAATDGGMVIHSWTCGPATGANPMPNQYLPGSCK
G

Nucleotide


Download         Length: 486 bp        

>NTDB_id=70539 EL386_RS03690 WP_126453548.1 713427..713912(-) (pilE) [Sulfuriflexus mobilis strain aks1]
ATGAAAACGATACAAAAAGGCTTTACCCTGATCGAACTCATGATCGTCGTTGCGATCATCGGTATCCTTGCCGCTGTGGC
ACTGCCGGCTTATCAGGACTACACCGTACGCGCCAAGGTCTCTGAAGTGATCCTGGCAGCCTCCTCATGCCGAACCGCCA
TCACCGAAGCGGTACAGTCAGCCGCCAACGTAAATATGGGCACTACCTTACAAAACGTGTGTAGCTTCACCCCAACGAAA
TATGTTGCCTCCGGTCTTGTAACTAGTGCTGGATTGATTGAAGTCACAGGTAATGCAACCAACCTAGGTGGCAGCACCAG
CGCTACTGCGAACACAATTAGAATGTCGCCACTTGTAACAAGTACTGCACGGGCTCTGAACGCAGCTACTGATGGCGGTA
TGGTCATTCACTCCTGGACATGTGGCCCCGCAACTGGAGCCAATCCAATGCCTAATCAGTATTTACCGGGTTCTTGTAAG
GGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(5-28)

Pilin (bacterial filament) (Pilin)

(36-160)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

40.816

100

0.497
  pilE Neisseria gonorrhoeae MS11

38.636

100

0.422
  pilA/pilA1 Eikenella corrodens VA1

38.922

100

0.404
  comP Acinetobacter baylyi ADP1

38.69

100

0.404
  pilA2 Legionella pneumophila str. Paris

41.026

96.894

0.398
  pilA2 Legionella pneumophila strain ERS1305867

40.385

96.894

0.391
  pilA Ralstonia pseudosolanacearum GMI1000

43.972

87.578

0.385
  pilA/pilAI Pseudomonas stutzeri DSM 10701

37.888

100

0.379

Multiple sequence alignment