Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   EL386_RS03685 Genome accession   NZ_AP018725
Coordinates   711410..713125 (-) Length   571 a.a.
NCBI ID   WP_172597607.1    Uniprot ID   -
Organism   Sulfuriflexus mobilis strain aks1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 706410..718125
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL386_RS03660 yacG 707343..707528 (-) 186 WP_126453538.1 DNA gyrase inhibitor YacG -
  EL386_RS03665 zapD 707535..708305 (-) 771 WP_126453540.1 cell division protein ZapD -
  EL386_RS03670 coaE 708413..709006 (-) 594 WP_126453542.1 dephospho-CoA kinase -
  EL386_RS03675 - 709295..710170 (-) 876 WP_126453544.1 prepilin peptidase -
  EL386_RS03680 pilC 710177..711406 (-) 1230 WP_126453546.1 type II secretion system F family protein Machinery gene
  EL386_RS03685 pilB 711410..713125 (-) 1716 WP_172597607.1 type IV-A pilus assembly ATPase PilB Machinery gene
  EL386_RS03690 pilE 713427..713912 (-) 486 WP_126453548.1 pilin Machinery gene
  EL386_RS15925 - 714285..714338 (-) 54 WP_420856730.1 hypothetical protein -
  EL386_RS03700 - 714555..715322 (+) 768 WP_126453550.1 hypothetical protein -
  EL386_RS03705 pilR 715413..716798 (-) 1386 WP_126453552.1 sigma-54-dependent transcriptional regulator Regulator

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62118.93 Da        Isoelectric Point: 4.9420

>NTDB_id=70538 EL386_RS03685 WP_172597607.1 711410..713125(-) (pilB) [Sulfuriflexus mobilis strain aks1]
MASINPQTTISGLARRLVHDGLLAEADAAVAHEQALKSKIPFVTHLVTKNLLDSLSIANAASQEFGVPLFDISTMDMETA
ATALVDEKLVRQHHALPLFKRGNRLYVAVSDPTNLQALDEIKFHVGANTEPVLVEEDKLSKAIEHAMEAQDTSLADLGDT
DLDDLDISSGDEAPQDDATDADIDDTPVVRFVNKVLLDAINKGASDIHFEPYEKTYRVRFRQDGILREIATPPVNLGSRL
CARLKVMSRLDTSERRIPQDGRMKMTLSKNRAIDFRVNTCPTLFGEKIVMRILDSSSAKLGIDALGYEEEQKELYLKALA
KPDGMILVTGPTGSGKTVSLYTGLNILNTEDRNISTAEDPVEINLAGINQVNVNNKVGLTFASALRAFLRQDPDIIMVGE
IRDLETAEIGIKAAQTGHLVLSTLHTNDAPQTLTRLVNMGVPPYNIASTVALIIAQRLARRLCTQCKKPVDIPKEALLEE
GFTEQDITGGLKVYGAVGCDQCAAGYKGRVGIYQVMPISDAMGRIIMEGGNAIQLADQAAKEGISDLRQSGLKKVKDGLT
SLEEINRVTKD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=70538 EL386_RS03685 WP_172597607.1 711410..713125(-) (pilB) [Sulfuriflexus mobilis strain aks1]
ATGGCATCAATCAACCCCCAAACCACGATCAGCGGCCTCGCCCGTCGGCTCGTCCATGACGGTCTGCTTGCCGAGGCCGA
TGCCGCTGTCGCGCACGAACAGGCGCTGAAGAGCAAGATCCCGTTTGTCACCCACCTGGTGACAAAGAATCTTCTCGATA
GCCTGAGTATCGCCAATGCCGCCTCACAGGAATTCGGCGTACCGCTGTTTGATATCAGTACCATGGACATGGAGACGGCC
GCCACCGCCCTGGTCGATGAAAAGCTGGTGCGTCAGCACCATGCCCTGCCGCTGTTCAAACGCGGTAACCGCCTCTATGT
CGCCGTCTCCGACCCCACCAACCTGCAGGCGCTGGATGAGATCAAATTCCACGTTGGCGCCAATACCGAGCCGGTACTGG
TTGAAGAAGACAAACTCAGCAAGGCGATTGAACACGCCATGGAGGCCCAGGACACCAGTCTCGCCGACCTCGGCGATACC
GACCTCGATGACCTGGATATCAGCAGTGGTGATGAGGCCCCGCAGGATGACGCCACTGACGCCGATATCGATGACACCCC
GGTGGTGCGCTTTGTGAACAAGGTCTTGCTCGATGCCATTAACAAGGGCGCCTCGGATATCCACTTCGAACCCTATGAAA
AGACCTACCGGGTACGTTTTCGCCAGGACGGCATCCTAAGAGAGATCGCCACGCCGCCGGTCAACCTCGGCAGCCGCCTG
TGTGCACGCCTGAAGGTCATGTCCCGCCTCGATACCTCGGAGCGCCGCATCCCGCAAGACGGGCGCATGAAGATGACCCT
GTCGAAGAACCGTGCCATCGATTTTCGTGTCAATACCTGCCCGACCCTGTTCGGCGAAAAGATCGTTATGCGTATTCTGG
ATTCGAGTAGTGCCAAACTCGGCATTGATGCACTCGGCTATGAAGAAGAGCAAAAGGAACTCTACCTGAAGGCCCTCGCC
AAACCGGATGGCATGATCCTCGTCACCGGCCCAACCGGCAGTGGTAAGACCGTGTCATTGTATACCGGTCTGAACATCCT
CAATACCGAAGACCGCAACATCTCCACCGCCGAAGACCCGGTAGAAATCAACCTGGCCGGTATTAATCAGGTCAATGTGA
ATAATAAGGTCGGCCTGACCTTTGCCAGCGCCCTGCGCGCCTTCCTGCGTCAGGACCCGGACATCATCATGGTCGGGGAA
ATCCGTGACCTAGAGACGGCCGAGATCGGCATCAAGGCCGCCCAGACCGGGCACCTGGTGTTATCCACTCTGCATACCAA
TGACGCCCCCCAGACCCTGACACGCCTGGTCAACATGGGGGTGCCGCCCTATAACATCGCCTCCACTGTAGCTTTAATTA
TTGCCCAGCGCCTGGCGCGACGCCTCTGCACACAGTGTAAAAAACCCGTCGATATCCCCAAAGAGGCCCTGCTCGAAGAG
GGCTTTACCGAACAGGATATCACTGGCGGCTTAAAGGTATATGGCGCGGTAGGCTGTGACCAATGTGCCGCGGGTTATAA
GGGCCGCGTCGGTATCTACCAGGTCATGCCGATTTCCGACGCGATGGGACGCATTATTATGGAAGGCGGTAACGCCATCC
AGCTCGCCGATCAGGCGGCCAAGGAAGGCATCAGTGACCTGCGCCAGTCAGGCCTGAAAAAGGTGAAGGATGGACTCACC
AGCCTCGAAGAAATTAACCGTGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)

Type II/IV secretion system protein (T2SSE)

(194-461)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.29

100

0.585
  pilB Acinetobacter baylyi ADP1

57.491

100

0.578
  pilB Legionella pneumophila strain ERS1305867

57.019

100

0.576
  pilF Neisseria gonorrhoeae MS11

50.973

98.949

0.504
  pilB Vibrio cholerae strain A1552

51.818

96.322

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

49.822

98.424

0.49
  pilB Vibrio campbellii strain DS40M4

47.965

98.949

0.475
  pilB/pilB1 Synechocystis sp. PCC 6803

40.154

90.718

0.364
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.319

92.644

0.364
  pilF Thermus thermophilus HB27

39.13

92.644

0.363

Multiple sequence alignment