Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   VCASEI_RS11950 Genome accession   NZ_AP018680
Coordinates   2548528..2550363 (-) Length   611 a.a.
NCBI ID   WP_089110418.1    Uniprot ID   -
Organism   Vibrio casei strain DSM 22364     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2543528..2555363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VCASEI_RS11930 - 2544167..2544994 (-) 828 WP_086960391.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  VCASEI_RS11935 - 2545134..2546543 (-) 1410 WP_086960392.1 AAA family ATPase -
  VCASEI_RS11940 aroB 2546644..2547729 (-) 1086 WP_086960393.1 3-dehydroquinate synthase -
  VCASEI_RS11945 aroK 2547759..2548277 (-) 519 WP_086960394.1 shikimate kinase AroK -
  VCASEI_RS11950 pilQ 2548528..2550363 (-) 1836 WP_089110418.1 type IV pilus secretin PilQ Machinery gene
  VCASEI_RS11955 pilP 2550400..2550912 (-) 513 WP_086960396.1 pilus assembly protein PilP Machinery gene
  VCASEI_RS11960 pilO 2550909..2551493 (-) 585 WP_086960397.1 type 4a pilus biogenesis protein PilO Machinery gene
  VCASEI_RS11965 pilN 2551493..2552095 (-) 603 WP_086960398.1 PilN domain-containing protein Machinery gene
  VCASEI_RS11970 pilM 2552085..2553110 (-) 1026 WP_086960399.1 type IV pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 611 a.a.        Molecular weight: 66276.19 Da        Isoelectric Point: 4.6389

>NTDB_id=70335 VCASEI_RS11950 WP_089110418.1 2548528..2550363(-) (pilQ) [Vibrio casei strain DSM 22364]
MHKGLTRTGLSRPFSLISSSIIACLLLTGNAAFAATTTEDSLQTTLKLQQSDRFTNEFVGLDFRLNKEKEGVIIVKLASS
ASAVDIKKDHKGLNIELIKTKVSDDNLYVIDVKDFSTPVSTVEVFRHDENTRLQASISNEFTYDYRLKGKLLEITITEVK
EKEISNTPKQQSALDKENELISINFQDIPVRNVLQLIADYNGFNLVVSDSVVGNLTLRLDGVPWQQVLEIILQVKGLDKR
VEGNVVLVAPKSELDEQQKKRLEQARMEEELGDLSSELLQINFAKASDIAAMIGGEGEISMLSSRGNITVDERTNSLLIR
DLASNINIIKGIVESLDIPVKQVQIEARIVTVNEGNMQDLGIRWGMSSTNGSFSAGGSIENNLANANPNGANGKGGVIDG
DQINFGGATQEVGIEDFLNVNLAATSANASSIAFQVAKLGSNTLLDLELSALQAESKAEIISSPRLITTNKQPAYIEQGT
EIPYLEASSSGATSISFKKAVLSLKVTPQITPDNRLVLDLSVTQDRPGAVVKTGDGEAVAIDTQRIGTQVLVDNGETVVL
GGIYQHSVQTSVDKVPLLGDIPYLGALFRRSYENYGKSELLIFVTPKVVLQ

Nucleotide


Download         Length: 1836 bp        

>NTDB_id=70335 VCASEI_RS11950 WP_089110418.1 2548528..2550363(-) (pilQ) [Vibrio casei strain DSM 22364]
ATGCATAAAGGATTAACGCGTACAGGTTTGAGCCGTCCATTCTCTCTGATATCAAGCAGTATCATTGCTTGCTTATTACT
GACAGGAAATGCTGCTTTCGCTGCCACAACAACGGAAGATTCACTTCAAACGACTTTGAAATTACAACAAAGTGATCGCT
TTACTAATGAGTTTGTTGGGTTGGATTTTCGTTTAAACAAAGAAAAAGAAGGCGTCATCATTGTTAAATTAGCATCTTCG
GCTTCTGCGGTTGATATTAAAAAAGACCACAAAGGATTAAACATTGAATTAATTAAAACGAAAGTGAGTGACGATAATTT
ATACGTTATCGATGTAAAAGATTTTTCAACACCGGTGAGTACTGTTGAAGTGTTTCGTCATGATGAAAACACTCGCTTGC
AAGCGTCAATCAGTAATGAGTTTACTTATGACTATCGCTTAAAAGGAAAGCTTTTAGAGATTACGATTACGGAAGTTAAA
GAAAAAGAAATCAGCAACACACCTAAACAGCAAAGTGCTTTAGATAAAGAAAATGAACTGATTTCTATTAATTTCCAAGA
TATTCCCGTGCGTAACGTGTTGCAACTCATTGCCGATTATAATGGTTTCAACTTGGTGGTTTCTGATTCCGTGGTAGGTA
ATTTAACCTTGCGTTTAGATGGTGTTCCTTGGCAACAAGTATTAGAGATTATTTTACAAGTTAAAGGGTTAGATAAACGG
GTTGAAGGTAATGTTGTTTTGGTTGCGCCTAAGAGTGAGTTAGATGAGCAACAGAAGAAACGCTTAGAACAGGCGCGAAT
GGAAGAAGAGTTAGGTGATTTAAGCTCTGAGTTGTTGCAAATTAATTTTGCTAAAGCGTCGGATATTGCCGCTATGATCG
GTGGTGAAGGTGAAATAAGCATGTTATCGAGCCGAGGTAATATTACGGTTGATGAACGTACAAACTCACTATTGATTCGT
GATTTGGCCTCTAATATCAATATTATTAAAGGGATAGTTGAATCCTTAGACATCCCTGTTAAACAGGTTCAAATAGAAGC
GAGAATCGTTACGGTCAACGAAGGCAACATGCAAGATCTTGGGATTCGTTGGGGGATGTCAAGCACAAATGGATCTTTCT
CTGCCGGAGGGAGTATTGAAAATAACCTCGCAAATGCAAATCCTAATGGTGCAAATGGAAAAGGTGGGGTCATTGATGGT
GACCAAATTAATTTTGGTGGCGCCACACAAGAGGTTGGTATTGAGGACTTCTTAAACGTGAATTTGGCCGCAACCAGTGC
GAATGCATCTAGTATTGCATTCCAAGTAGCAAAGCTTGGTTCAAATACATTATTGGATTTAGAGCTTTCTGCGCTGCAAG
CAGAATCAAAAGCGGAAATTATTTCAAGCCCACGTTTAATCACGACAAATAAGCAACCAGCCTATATTGAACAAGGTACG
GAGATTCCTTATTTAGAAGCCTCTTCAAGTGGTGCGACGTCGATTAGTTTTAAAAAAGCGGTATTAAGTTTGAAGGTGAC
ACCGCAAATTACACCGGATAACCGATTGGTGCTGGATTTGAGTGTGACTCAAGATAGGCCCGGTGCTGTGGTTAAAACTG
GAGATGGTGAAGCTGTCGCTATAGATACTCAACGAATTGGCACACAAGTACTGGTAGATAATGGTGAAACTGTGGTGTTG
GGAGGGATTTATCAACATTCAGTACAAACCAGTGTTGATAAGGTGCCATTACTCGGAGATATTCCATATCTTGGAGCTTT
ATTCCGCCGTAGTTATGAAAATTATGGTAAGAGTGAATTGCTGATTTTTGTTACCCCAAAAGTTGTTTTACAGTAA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(451-609)

Bacterial type II/III secretion system short domain (Secretin_N)

(278-342)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

72.122

90.998

0.656
  pilQ Vibrio cholerae strain A1552

72.122

90.998

0.656
  pilQ Vibrio campbellii strain DS40M4

71.275

91.162

0.65
  pilQ Legionella pneumophila strain Lp02

40.142

92.144

0.37
  pilQ Legionella pneumophila strain ERS1305867

40.142

92.144

0.37
  pilQ Pseudomonas aeruginosa PAK

39.677

91.162

0.362

Multiple sequence alignment