Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   NHN13_RS13065 Genome accession   NZ_CP099979
Coordinates   1445622..1445861 (-) Length   79 a.a.
NCBI ID   WP_230207119.1    Uniprot ID   -
Organism   Enterococcus faecium strain WN-18     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1440622..1450861
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NHN13_RS06890 (NHN13_06875) - 1440640..1440909 (+) 270 WP_002289125.1 hypothetical protein -
  NHN13_RS06895 (NHN13_06880) rpsT 1441309..1441563 (+) 255 WP_002292188.1 30S ribosomal protein S20 -
  NHN13_RS06900 (NHN13_06885) holA 1441648..1442694 (-) 1047 WP_002329129.1 DNA polymerase III subunit delta -
  NHN13_RS06905 (NHN13_06890) - 1442731..1444909 (-) 2179 Protein_1369 ComEC/Rec2 family competence protein -
  NHN13_RS06910 (NHN13_06895) - 1444902..1445441 (-) 540 WP_002292191.1 ComE operon protein 2 -
  NHN13_RS13065 (NHN13_06900) comEA 1445622..1445861 (-) 240 WP_230207119.1 ComEA family DNA-binding protein Machinery gene
  NHN13_RS13070 (NHN13_06905) - 1445995..1446201 (-) 207 WP_230207120.1 hypothetical protein -
  NHN13_RS06925 (NHN13_06910) - 1446630..1447673 (-) 1044 WP_002292194.1 SepM family pheromone-processing serine protease -
  NHN13_RS06930 (NHN13_06915) coaD 1447663..1448154 (-) 492 WP_002292195.1 pantetheine-phosphate adenylyltransferase -
  NHN13_RS06935 (NHN13_06920) rsmD 1448151..1448711 (-) 561 WP_002292196.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  NHN13_RS06940 (NHN13_06925) - 1448842..1449180 (-) 339 WP_002292197.1 YlbG family protein -
  NHN13_RS06945 (NHN13_06930) - 1449170..1449601 (-) 432 WP_002342659.1 YlbF family regulator -
  NHN13_RS06950 (NHN13_06935) - 1449591..1450715 (-) 1125 WP_002292199.1 CAP-associated domain-containing protein -

Sequence

Protein


Download         Length: 79 a.a.        Molecular weight: 8622.66 Da        Isoelectric Point: 4.7854

>NTDB_id=703139 NHN13_RS13065 WP_230207119.1 1445622..1445861(-) (comEA) [Enterococcus faecium strain WN-18]
MASSEGKQSTADSEPSKININTASPSELQQIPGIGSVKAQEIIRFREENGSFQKVEDLQEISGIGEKTVEKLKNFVTIK

Nucleotide


Download         Length: 240 bp        

>NTDB_id=703139 NHN13_RS13065 WP_230207119.1 1445622..1445861(-) (comEA) [Enterococcus faecium strain WN-18]
TTGGCATCCTCAGAAGGAAAACAATCTACAGCAGATTCCGAGCCTTCAAAAATAAATATCAATACAGCCAGTCCCTCAGA
ACTCCAGCAGATTCCTGGGATCGGATCAGTCAAGGCGCAAGAAATCATTCGATTTAGAGAAGAAAACGGATCCTTCCAGA
AAGTGGAAGACTTGCAGGAGATCTCTGGTATCGGTGAAAAGACAGTTGAAAAACTAAAAAACTTCGTTACAATTAAATAG

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(16-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Lactococcus lactis subsp. cremoris KW2

50.649

97.468

0.494
  comEA/celA/cilE Streptococcus mitis SK321

49.351

97.468

0.481
  comEA Bacillus subtilis subsp. subtilis str. 168

56.452

78.481

0.443
  comEA/celA/cilE Streptococcus pneumoniae R6

51.515

83.544

0.43
  comEA/celA/cilE Streptococcus pneumoniae Rx1

51.515

83.544

0.43
  comEA/celA/cilE Streptococcus pneumoniae D39

51.515

83.544

0.43
  comEA/celA/cilE Streptococcus mitis NCTC 12261

48.571

88.608

0.43
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

48.571

88.608

0.43
  comEA Streptococcus thermophilus LMD-9

46.479

89.873

0.418
  comEA/comE1 Glaesserella parasuis strain SC1401

51.613

78.481

0.405
  comEA Staphylococcus aureus N315

44.776

84.81

0.38
  comEA Staphylococcus aureus MW2

44.776

84.81

0.38
  comEA Latilactobacillus sakei subsp. sakei 23K

41.429

88.608

0.367