Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NI375_RS12445 Genome accession   NZ_CP099956
Coordinates   2737836..2738306 (+) Length   156 a.a.
NCBI ID   WP_182032768.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc15A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2732836..2743306
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI375_RS12430 (NI375_12630) pdhR 2734869..2735636 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NI375_RS12435 (NI375_12635) ampD 2736042..2736593 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI375_RS12440 (NI375_12640) nadC 2736686..2737573 (+) 888 WP_308386869.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI375_RS12445 (NI375_12645) pilA 2737836..2738306 (+) 471 WP_182032768.1 pilin Machinery gene
  NI375_RS12450 (NI375_12650) pilB 2738307..2739992 (+) 1686 WP_308386870.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI375_RS12455 (NI375_12655) pilC 2740016..2741239 (+) 1224 WP_213867260.1 type II secretion system F family protein Machinery gene
  NI375_RS12460 (NI375_12660) pilD 2741304..2742173 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  NI375_RS12465 (NI375_12665) coaE 2742174..2742788 (+) 615 WP_017635920.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 156 a.a.        Molecular weight: 16089.31 Da        Isoelectric Point: 6.2551

>NTDB_id=702932 NI375_RS12445 WP_182032768.1 2737836..2738306(+) (pilA) [Vibrio alginolyticus strain Isc15A]
MKNSKQKKQQGFTLIELMIVVAVIGVLAAIAMPQYQKYVAKSEVASALATLTGAKTNVEAYTVENGLFPDGSSQDQTTTD
LGIPTMPLGSVAFTPSGTGDGGTMAFTFFTTASAGVSSLVSGKGFTLTRADTSGQWTCATATTSGVESELLPKSCK

Nucleotide


Download         Length: 471 bp        

>NTDB_id=702932 NI375_RS12445 WP_182032768.1 2737836..2738306(+) (pilA) [Vibrio alginolyticus strain Isc15A]
ATGAAAAACAGTAAACAGAAAAAACAACAGGGTTTTACTCTGATTGAATTAATGATTGTGGTGGCAGTAATTGGTGTCTT
GGCGGCAATTGCAATGCCACAATACCAGAAGTACGTTGCTAAAAGTGAAGTAGCCTCAGCATTGGCAACGCTAACCGGAG
CAAAAACGAACGTTGAGGCATACACTGTTGAAAACGGACTTTTCCCAGATGGCTCTAGTCAGGATCAAACAACTACTGAT
CTAGGTATTCCAACAATGCCTCTTGGAAGCGTTGCGTTTACTCCTAGCGGCACAGGCGATGGTGGAACAATGGCATTTAC
ATTTTTCACGACAGCAAGCGCTGGCGTAAGTAGCCTTGTTTCAGGTAAAGGTTTTACCCTTACACGAGCTGATACTTCAG
GGCAGTGGACGTGTGCAACAGCCACAACTAGCGGCGTTGAGTCAGAACTCCTTCCAAAGTCTTGCAAGTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(7-32)

Pilin (bacterial filament) (Pilin)

(40-156)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

48.101

100

0.487
  pilA Vibrio cholerae strain A1552

48.101

100

0.487
  pilA Vibrio cholerae C6706

48.101

100

0.487
  pilA Pseudomonas aeruginosa PAK

42.208

98.718

0.417
  pilA Acinetobacter baumannii strain A118

40.645

99.359

0.404
  pilA Vibrio parahaemolyticus RIMD 2210633

44.928

88.462

0.397
  pilA/pilAI Pseudomonas stutzeri DSM 10701

38.816

97.436

0.378
  pilA/pilA1 Eikenella corrodens VA1

36.25

100

0.372