Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   NI375_RS12450 Genome accession   NZ_CP099956
Coordinates   2738307..2739992 (+) Length   561 a.a.
NCBI ID   WP_308386870.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc15A     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2733307..2744992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI375_RS12430 (NI375_12630) pdhR 2734869..2735636 (-) 768 WP_005379989.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  NI375_RS12435 (NI375_12635) ampD 2736042..2736593 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI375_RS12440 (NI375_12640) nadC 2736686..2737573 (+) 888 WP_308386869.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI375_RS12445 (NI375_12645) pilA 2737836..2738306 (+) 471 WP_182032768.1 pilin Machinery gene
  NI375_RS12450 (NI375_12650) pilB 2738307..2739992 (+) 1686 WP_308386870.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI375_RS12455 (NI375_12655) pilC 2740016..2741239 (+) 1224 WP_213867260.1 type II secretion system F family protein Machinery gene
  NI375_RS12460 (NI375_12660) pilD 2741304..2742173 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  NI375_RS12465 (NI375_12665) coaE 2742174..2742788 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  NI375_RS12470 (NI375_12670) zapD 2742816..2743556 (+) 741 WP_005373086.1 cell division protein ZapD -
  NI375_RS12475 (NI375_12675) yacG 2743715..2743909 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  NI375_RS12480 (NI375_12680) rplS 2744565..2744918 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62204.02 Da        Isoelectric Point: 5.5283

>NTDB_id=702933 NI375_RS12450 WP_308386870.1 2738307..2739992(+) (pilB) [Vibrio alginolyticus strain Isc15A]
MHTKLSTVLRQAGILTYSQEELLLEQAKASGISMPEALLNSGFFQSYELTEHLSSVFGLSCTSLNQYEYASLCQTLGLRD
LITRHNALPLNRTSSTLILAVADPTNQQAEDDFRFATGLQVELVLADFRQLSAAIRRLYGRSLSHEKSGLKEINQEELAN
LVDVGADEIDNIEDLSQDESPVSRYINQILLDAVRKGASDIHFEPYEKMYRVRLRCDGILIETQQPPSHLSRRLSARIKI
LSKLDIAERRLPQDGRIKLKLNQDTAIDMRVSTLPTLFGEKIVLRLLDSSSASLDIDKLGYSEQQKQLYLDALCRPQGMI
LMTGPTGSGKTVSLYTGLSILNKPEINISTAEDPVEINLSGINQVQVQPKIGFGFAEALRSFLRQDPDVVMVGEIRDLDT
AEIAIKASQTGHLVLSTLHTNSAAETVIRLSNMGVESFNLASSLSLIIAQRLARKLCSYCKQSQELTVQLQHLGIQASDN
IFKANPDGCNECTHGYSGRTGIYEVMRFDEFLSEALIKGASVHELEKLAIANGMSTLQMSGIEKLKQGITSFSELQRVLY
F

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=702933 NI375_RS12450 WP_308386870.1 2738307..2739992(+) (pilB) [Vibrio alginolyticus strain Isc15A]
ATGCATACAAAACTCTCAACAGTTCTTCGTCAAGCAGGAATACTGACATATTCCCAAGAAGAACTGTTACTTGAGCAAGC
TAAAGCTTCTGGTATATCAATGCCGGAGGCTTTGCTCAATTCTGGCTTTTTCCAATCTTATGAATTAACGGAGCACCTAA
GCTCTGTTTTTGGGCTTTCTTGCACTTCTCTCAATCAATACGAATACGCTTCGTTATGCCAAACGCTAGGTTTGCGAGAT
TTGATCACTCGGCACAACGCTCTGCCGCTTAATCGCACCTCTTCAACTTTGATTCTTGCTGTTGCCGACCCCACCAACCA
ACAAGCAGAAGATGATTTTCGCTTTGCCACTGGTTTACAGGTTGAATTGGTCTTGGCGGATTTTCGTCAACTCAGCGCGG
CAATTCGACGCTTATATGGTCGTTCATTGAGTCATGAGAAGTCCGGGTTAAAAGAGATAAACCAAGAAGAGTTAGCCAAT
CTGGTTGACGTGGGCGCAGATGAAATCGACAACATTGAAGACTTGAGCCAAGATGAGTCTCCTGTTAGCCGTTACATCAA
CCAAATTCTGTTGGACGCTGTGCGCAAAGGCGCATCCGATATCCACTTTGAACCTTATGAAAAGATGTACCGCGTGCGCC
TACGCTGTGATGGCATCTTAATTGAAACACAGCAGCCACCAAGCCACTTGAGTCGCCGCTTATCCGCTCGTATCAAAATC
CTCTCTAAGCTTGATATTGCCGAAAGGCGCTTACCACAAGATGGGCGTATTAAGCTCAAGTTGAATCAAGACACCGCAAT
TGATATGCGTGTATCCACGCTACCTACTCTATTCGGAGAGAAAATTGTATTACGACTGCTCGACAGCAGCTCTGCGTCAC
TGGATATCGACAAGCTTGGTTATAGCGAACAACAAAAACAATTGTACTTGGATGCGCTGTGTCGCCCACAAGGCATGATT
CTGATGACAGGCCCAACTGGGAGCGGGAAAACCGTTTCTCTGTACACTGGGCTGAGTATTCTCAATAAACCAGAAATCAA
CATTTCCACGGCAGAAGACCCAGTAGAAATTAACTTATCGGGCATTAACCAAGTTCAAGTTCAGCCTAAGATTGGTTTTG
GTTTTGCCGAAGCCCTGCGCTCTTTTCTTCGTCAAGACCCCGATGTGGTCATGGTCGGTGAGATTCGTGACTTAGACACT
GCGGAAATCGCCATAAAAGCATCGCAAACCGGTCACTTAGTACTTTCAACCTTGCATACCAATTCTGCGGCTGAAACGGT
TATTCGTTTATCAAACATGGGCGTAGAAAGCTTTAACTTGGCCTCATCTCTTAGTTTGATTATTGCCCAGCGCTTAGCCC
GTAAACTGTGCTCGTACTGCAAACAATCACAAGAGCTTACCGTTCAGCTACAACATCTCGGAATTCAAGCAAGCGACAAC
ATTTTTAAAGCCAATCCCGATGGATGCAATGAGTGTACCCATGGTTATTCCGGCCGAACGGGGATTTATGAAGTGATGCG
CTTTGATGAATTTCTTTCCGAAGCGCTTATTAAAGGCGCGTCGGTACATGAACTAGAAAAACTCGCCATCGCAAATGGTA
TGAGCACGTTACAAATGTCGGGCATAGAAAAACTCAAACAAGGGATCACAAGTTTTAGTGAACTACAGCGCGTACTTTAT
TTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

93.226

100

0.932
  pilB Vibrio campbellii strain DS40M4

92.335

100

0.923
  pilB Vibrio cholerae strain A1552

73.488

100

0.736
  pilB Acinetobacter baumannii D1279779

51.731

92.692

0.48
  pilB Legionella pneumophila strain ERS1305867

49.355

96.791

0.478
  pilB Acinetobacter baylyi ADP1

48.708

96.613

0.471
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.449
  pilB/pilB1 Synechocystis sp. PCC 6803

36.745

100

0.39
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.315

99.643

0.362
  pilF Thermus thermophilus HB27

35.943

100

0.36