Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   NI375_RS12455 Genome accession   NZ_CP099956
Coordinates   2740016..2741239 (+) Length   407 a.a.
NCBI ID   WP_213867260.1    Uniprot ID   -
Organism   Vibrio alginolyticus strain Isc15A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2735016..2746239
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NI375_RS12435 (NI375_12635) ampD 2736042..2736593 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  NI375_RS12440 (NI375_12640) nadC 2736686..2737573 (+) 888 WP_308386869.1 carboxylating nicotinate-nucleotide diphosphorylase -
  NI375_RS12445 (NI375_12645) pilA 2737836..2738306 (+) 471 WP_182032768.1 pilin Machinery gene
  NI375_RS12450 (NI375_12650) pilB 2738307..2739992 (+) 1686 WP_308386870.1 type IV-A pilus assembly ATPase PilB Machinery gene
  NI375_RS12455 (NI375_12655) pilC 2740016..2741239 (+) 1224 WP_213867260.1 type II secretion system F family protein Machinery gene
  NI375_RS12460 (NI375_12660) pilD 2741304..2742173 (+) 870 WP_012841418.1 A24 family peptidase Machinery gene
  NI375_RS12465 (NI375_12665) coaE 2742174..2742788 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  NI375_RS12470 (NI375_12670) zapD 2742816..2743556 (+) 741 WP_005373086.1 cell division protein ZapD -
  NI375_RS12475 (NI375_12675) yacG 2743715..2743909 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  NI375_RS12480 (NI375_12680) rplS 2744565..2744918 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  NI375_RS12485 (NI375_12685) trmD 2744960..2745721 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45315.47 Da        Isoelectric Point: 10.3992

>NTDB_id=702934 NI375_RS12455 WP_213867260.1 2740016..2741239(+) (pilC) [Vibrio alginolyticus strain Isc15A]
MKTTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLVQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFTSA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=702934 NI375_RS12455 WP_213867260.1 2740016..2741239(+) (pilC) [Vibrio alginolyticus strain Isc15A]
ATGAAAACCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCAGGCAAAAAGACGTCTGGACAAAC
TCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAACACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TCTCGTTTCTCACTAAACTCAGCCATCGCGTAAAAGGCAGAGACATCACGGTGTTTACCCGTCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTAGTTCAGGCTTTAAAATTGGTATCAGAAAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTAACACGCGCCGTCGAAGCGGGTACGCCTATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACCGACCTTATCGCAACAGGTGAGCAATCCGGTAACTTAGCGGAAGTATTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTCGCGTTGGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGATTTACCTTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCACCTCGGCG
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTTCTGGGCCCTGT
ATTATCAAAAGCTGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGATAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCGCTAATTATCGTTTTTTTAGGCATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

87.224

100

0.872
  pilC Vibrio cholerae strain A1552

73.827

99.509

0.735
  pilC Acinetobacter baylyi ADP1

43

98.28

0.423
  pilC Legionella pneumophila strain ERS1305867

40.732

100

0.41
  pilC Acinetobacter baumannii D1279779

41.75

98.28

0.41
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398
  pilG Neisseria meningitidis 44/76-A

39.901

99.754

0.398