Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   NH673_RS05640 Genome accession   NZ_CP099727
Coordinates   1254860..1256122 (-) Length   420 a.a.
NCBI ID   WP_080999542.1    Uniprot ID   -
Organism   Pseudomonas putida strain PCL1760     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1249860..1261122
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NH673_RS05625 (NH673_05625) - 1251538..1253127 (-) 1590 WP_019439128.1 AAA family ATPase -
  NH673_RS05630 (NH673_05630) aroB 1253143..1254240 (-) 1098 WP_053867802.1 3-dehydroquinate synthase -
  NH673_RS05635 (NH673_05635) aroK 1254337..1254855 (-) 519 WP_010955625.1 shikimate kinase AroK -
  NH673_RS05640 (NH673_05640) pilQ 1254860..1256122 (-) 1263 WP_080999542.1 secretin N-terminal domain-containing protein Machinery gene
  NH673_RS05645 (NH673_05645) - 1256046..1257047 (-) 1002 WP_053867804.1 pilus assembly protein PilP -
  NH673_RS05650 (NH673_05650) - 1257059..1257595 (-) 537 WP_010955628.1 PilN domain-containing protein -
  NH673_RS05655 (NH673_05655) pilM 1257592..1258473 (-) 882 WP_053867805.1 pilus assembly protein PilM -

Sequence

Protein


Download         Length: 420 a.a.        Molecular weight: 45713.44 Da        Isoelectric Point: 4.9919

>NTDB_id=701339 NH673_RS05640 WP_080999542.1 1254860..1256122(-) (pilQ) [Pseudomonas putida strain PCL1760]
MKTWMNYLLATLLSIPLASATPYQGEPLSLNFQDVEVRAVLQVLADYAGVNLVASDAVKGSVTLRLQDVPWDQALDLVLR
SKGLSRRQEGNVLLVAPAAEFAAQSADARVSQALEAHLQPLRRELLPIHHAKAADLAQLLLAGLEGDGIPPASLSVDERT
NTLVVYLPADRLAEMRQLVAQLDVPVRQVAIEARIVEANVDYEKSLGVRWGGPLYGEKLRPGKELFVDLGLERAGSSIGL
GLLRGDVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSQSGATSVSFREASLSLEVTPQITPDNKV
IMTVRVTKDEPDYVNALNNVPPIRKNEVNAKVRVADGETIVIGGVYSTSQNNVVDKVPFFGDLPYVGRLFRRDALQEKKS
ELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1263 bp        

>NTDB_id=701339 NH673_RS05640 WP_080999542.1 1254860..1256122(-) (pilQ) [Pseudomonas putida strain PCL1760]
ATGAAAACGTGGATGAACTACCTGTTGGCAACCCTGCTGTCGATACCGCTGGCTTCGGCGACGCCTTACCAGGGTGAACC
CCTGTCGCTGAACTTCCAGGATGTTGAGGTGCGTGCGGTGCTGCAGGTGCTGGCTGACTACGCTGGCGTCAACCTGGTCG
CCAGTGATGCAGTAAAAGGCAGTGTCACTTTGCGTTTGCAGGACGTGCCGTGGGATCAGGCGCTTGACCTGGTGCTGCGC
AGCAAGGGCCTGTCCCGGCGCCAGGAGGGCAATGTGCTGTTGGTGGCACCTGCAGCCGAGTTTGCCGCGCAATCGGCAGA
CGCCCGGGTGAGTCAGGCGCTGGAGGCGCACCTGCAGCCATTGCGTCGAGAGCTGCTGCCCATTCACCACGCCAAGGCCG
CCGATCTTGCGCAGTTGCTGCTGGCGGGCCTGGAGGGTGACGGCATCCCTCCGGCTAGCTTGAGTGTCGATGAGCGCACC
AATACGTTGGTGGTGTATCTGCCCGCTGACCGCCTGGCCGAGATGCGCCAGCTGGTGGCGCAGTTGGATGTGCCGGTGCG
TCAGGTAGCGATCGAGGCGCGTATCGTCGAAGCCAACGTCGATTATGAGAAAAGCCTGGGGGTACGCTGGGGTGGGCCGC
TGTACGGCGAGAAACTGCGGCCCGGCAAGGAGCTGTTCGTCGACCTCGGTCTGGAGCGTGCCGGCAGCAGTATCGGCCTG
GGTTTGTTGCGCGGTGACGTGTTGCTGGACCTGGAGCTCAGTGCCATGGAAAAAAGCGGCAACGGCGAGATCATTTCGCA
GCCCAAGGTGGTCACGGCCGACAAGGAAACGGCGAGGATACTCAAGGGTACCGAGGTGCCTTACCAGGAAACGAGCCAAA
GCGGCGCTACCTCGGTATCGTTTCGCGAAGCCTCGCTGTCGCTGGAAGTAACGCCACAGATCACCCCGGACAACAAGGTG
ATCATGACCGTCAGGGTGACCAAGGACGAGCCGGATTACGTCAATGCCTTGAACAACGTACCGCCGATCCGCAAGAACGA
GGTCAATGCCAAGGTGCGCGTGGCTGACGGCGAAACGATCGTGATCGGTGGCGTCTACTCGACTTCGCAAAACAATGTGG
TCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAAAAATCC
GAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(51-97)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-188)

Bacterial type II and III secretion system protein (Secretin)

(255-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

59.174

100

0.614
  comQ Acinetobacter baylyi ADP1

42.316

100

0.452
  pilQ Acinetobacter baumannii D1279779

40.998

100

0.45
  pilQ Acinetobacter baumannii strain A118

40.998

100

0.45
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

42.389

100

0.431
  pilQ Vibrio cholerae strain A1552

42.389

100

0.431
  pilQ Legionella pneumophila strain ERS1305867

42.788

99.048

0.424
  pilQ Legionella pneumophila strain Lp02

42.788

99.048

0.424
  pilQ Vibrio campbellii strain DS40M4

40.69

100

0.421
  pilQ Neisseria meningitidis M1080

35.765

100

0.362
  pilQ Neisseria gonorrhoeae strain FA1090

35.765

100

0.362
  pilQ Neisseria meningitidis MC58

35.765

100

0.362