NTDB

Overview

Name	comGF/cglF	Type	Machinery gene
Locus tag	NEE09_RS01235	Genome accession	NZ_CP099641
Coordinates	240382..240843 (+)	Length	153 a.a.
NCBI ID	WP_000250531.1	Uniprot ID	-
Organism	Streptococcus pneumoniae strain ATCC 700671
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 235382..245843

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NEE09_RS01200	-	235877..236935 (+)	1059	WP_000649468.1	zinc-dependent alcohol dehydrogenase family protein	-
NEE09_RS01205	-	237086..237451 (+)	366	WP_000286415.1	DUF1033 family protein	-
NEE09_RS01210	comGA/cglA/cilD	237527..238468 (+)	942	WP_000249536.1	competence type IV pilus ATPase ComGA	Machinery gene
NEE09_RS01215	comGB/cglB	238416..239432 (+)	1017	WP_078130737.1	competence type IV pilus assembly protein ComGB	Machinery gene
NEE09_RS01220	comGC/cglC	239434..239757 (+)	324	WP_000738628.1	comG operon protein ComGC	Machinery gene
NEE09_RS01225	comGD/cglD	239720..240154 (+)	435	WP_033606693.1	competence type IV pilus minor pilin ComGD	Machinery gene
NEE09_RS01230	comGE/cglE	240117..240419 (+)	303	WP_000413382.1	competence type IV pilus minor pilin ComGE	Machinery gene
NEE09_RS01235	comGF/cglF	240382..240843 (+)	462	WP_000250531.1	competence type IV pilus minor pilin ComGF	Machinery gene
NEE09_RS01240	comGG/cglG	240821..241267 (+)	447	WP_001809944.1	competence type IV pilus minor pilin ComGG	Machinery gene
NEE09_RS01245	-	241370..241962 (+)	593	Protein_226	class I SAM-dependent methyltransferase	-
NEE09_RS01250	comYH	242023..242976 (+)	954	WP_000345126.1	class I SAM-dependent methyltransferase	Machinery gene
NEE09_RS01255	-	243027..244217 (+)	1191	WP_000167765.1	acetate kinase	-
NEE09_RS01260	-	244218..244349 (+)	132	WP_000768904.1	hypothetical protein	-
NEE09_RS01265	rnpA	244366..244737 (+)	372	WP_000739246.1	ribonuclease P protein component	-
NEE09_RS01270	-	244712..245536 (+)	825	WP_000727904.1	membrane protein insertase YidC	-

Sequence

Protein

Download Length: 153 a.a. Molecular weight: 17849.37 Da Isoelectric Point: 6.4832

                                >NTDB_id=701173 NEE09_RS01235 WP_000250531.1 240382..240843(+) (comGF/cglF) [Streptococcus pneumoniae strain ATCC 700671]

MVQNSCWQSKSHKVKAFTLLESLLALIVISGGLLLFQAMSQLLISEVRYQQQSEQKEWLLFVDQLEVELDRSQFEKVEGN

RLYMKQDGKDIAIGKSKSDDFRKTDASGRGYQPMVYGLKSAQITEDNQLVHFRFQFQKGLEREFIYRVEKEES

Nucleotide

Download Length: 462 bp

                                >NTDB_id=701173 NEE09_RS01235 WP_000250531.1 240382..240843(+) (comGF/cglF) [Streptococcus pneumoniae strain ATCC 700671]

ATGGTTCAGAACAGTTGTTGGCAATCAAAGAGCCATAAGGTCAAGGCTTTTACCTTGTTAGAATCCCTGCTTGCCCTCAT

TGTCATCAGTGGGGGATTACTCCTTTTTCAAGCTATGAGTCAGCTCCTCATTTCAGAAGTTCGCTACCAGCAACAAAGCG

AGCAAAAGGAGTGGCTCTTGTTTGTGGACCAACTTGAGGTAGAATTAGACCGTTCGCAGTTCGAAAAAGTAGAAGGCAAT

CGCCTATACATGAAGCAAGATGGCAAGGACATCGCCATCGGTAAGTCAAAGTCAGACGATTTTCGTAAAACCGATGCTAG

TGGACGGGGGTATCAGCCTATGGTTTATGGCCTCAAATCAGCTCAGATTACAGAGGACAATCAACTGGTTCATTTTCGTT

TCCAGTTTCAAAAAGGCTTAGAAAGGGAGTTCATCTATCGTGTGGAAAAAGAAGAAAGTTAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(45-141)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF/cglF	Streptococcus pneumoniae Rx1	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae D39	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae R6	95.425	100	0.954
comGF/cglF	Streptococcus pneumoniae TIGR4	95.425	100	0.954
comGF/cglF	Streptococcus mitis SK321	92.81	100	0.928
comGF/cglF	Streptococcus mitis NCTC 12261	91.503	100	0.915

Detailed information NTG0701173