Detailed information NTG0700896

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: NF676_RS12475
• Genome accession: NZ_CP099598
• Coordinates: 2682962..2684071 (+)
• Length: 369 a.a.
• NCBI ID: WP_252887815.1
• Uniprot ID: -
• Organism: Pseudomonas siliginis strain OTU6BANIB1
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2677962..2689071

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
NF676_RS12460 (NF676_12460)	-	2678950..2679870 (+)	921	WP_150635519.1	sugar kinase	-
NF676_RS12465 (NF676_12465)	-	2679987..2680262 (-)	276	WP_007912322.1	peptidylprolyl isomerase	-
NF676_RS12470 (NF676_12470)	-	2680315..2682852 (-)	2538	WP_252887814.1	PAS domain-containing protein	-
NF676_RS12475 (NF676_12475)	pilU	2682962..2684071 (+)	1110	WP_252887815.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
NF676_RS12480 (NF676_12480)	-	2684204..2687962 (+)	3759	WP_252887816.1	hypothetical protein	-

Sequence

Protein

• Length: 369 a.a.
• Molecular weight: 40879.75 Da
• Isoelectric Point: 6.8410

>NTDB_id=700896 NF676_RS12475 WP_252887815.1 2682962..2684071(+) (pilU) [Pseudomonas siliginis strain OTU6BANIB1]
MEIDALLSTLSQKNGSDLFLSTGAPPSARIDGVLTALSDQPFKPGETAAIATSLMDAEQRREFDRDLEMNLAISRTGVGR
FRVNIFKQRNDVSIVIRNVKLDIPRFEDLKLPPVLLETVMLKRGLILFVGATSSGKSTSLAALIDHRNRHSAGHIVTIED
PIEYIHRHKRSIINQREVGVDTRSFHAALKNTLRQAPDVVLIGEIRDRETMEHALSFADTGHLVLSTLHATNANQALDRI
INMFPEEKREQLLQTLGNNLKACVSQRLVRTVDGQRRAAVEILLGTPTIADLVRRGQFEELKPLMEKSFELGMQTFDAAL
FALVAEGAVSEQEALKNADSVNNLKLRIKLQHDQASVNNSGAGEWGLMD

Nucleotide

• Length: 1110 bp

>NTDB_id=700896 NF676_RS12475 WP_252887815.1 2682962..2684071(+) (pilU) [Pseudomonas siliginis strain OTU6BANIB1]
ATGGAAATCGATGCACTGTTGTCAACATTGTCGCAAAAAAACGGTTCGGATCTCTTCCTTTCCACCGGCGCACCACCGAG
CGCTCGCATTGATGGTGTGCTGACGGCGCTCAGCGATCAGCCATTCAAACCTGGCGAAACCGCGGCCATCGCCACCTCCC
TGATGGACGCCGAGCAGCGCCGGGAGTTCGACCGGGATCTTGAAATGAACCTAGCAATCTCCCGAACGGGCGTCGGCCGG
TTCCGGGTGAACATTTTCAAGCAGCGCAACGACGTTTCGATCGTTATCCGCAACGTCAAGCTCGACATCCCGCGGTTCGA
AGACTTGAAACTGCCGCCGGTGTTGCTGGAAACGGTGATGCTCAAACGTGGCCTGATTCTTTTTGTCGGCGCTACCAGTT
CGGGCAAGTCGACCTCCCTGGCGGCGCTCATCGATCATCGCAACCGTCACAGTGCCGGACACATTGTTACCATCGAAGAC
CCGATCGAATATATCCATCGGCACAAGCGCTCGATCATCAATCAGCGTGAGGTTGGCGTCGATACGCGCAGCTTTCATGC
TGCACTGAAGAACACTTTACGCCAGGCACCGGACGTCGTCCTGATTGGTGAGATCCGTGACCGCGAAACCATGGAGCACG
CGCTCTCGTTTGCCGATACAGGGCATCTGGTGTTGTCGACATTACATGCGACCAACGCCAATCAGGCGCTGGATCGCATC
ATCAATATGTTTCCCGAGGAGAAGCGCGAACAGCTTTTGCAGACATTGGGCAACAACCTCAAAGCGTGTGTCTCGCAGCG
TCTGGTCCGCACTGTCGACGGGCAAAGGCGCGCGGCGGTCGAGATTCTTCTGGGCACGCCGACCATCGCTGATCTGGTCC
GCCGCGGTCAGTTCGAGGAACTCAAACCGCTGATGGAAAAATCATTCGAACTCGGTATGCAGACCTTTGACGCAGCACTG
TTTGCGCTGGTTGCAGAAGGTGCTGTCAGCGAGCAGGAAGCATTGAAGAATGCCGATTCGGTGAATAACCTGAAGTTGCG
AATTAAACTTCAGCACGATCAAGCGAGTGTTAACAATTCAGGCGCCGGCGAGTGGGGTTTAATGGACTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (58-271)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	55.556	95.122	0.528
pilU	Acinetobacter baylyi ADP1	53.409	95.393	0.509
pilU	Vibrio cholerae strain A1552	51.429	94.851	0.488
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.003	91.87	0.377
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	40.841	90.244	0.369
pilT	Vibrio cholerae strain A1552	40.841	90.244	0.369
pilT	Pseudomonas stutzeri DSM 10701	39.535	93.225	0.369
pilT	Pseudomonas aeruginosa PAK	39.244	93.225	0.366
pilT	Acinetobacter baumannii D1279779	40	90.786	0.363
pilT	Acinetobacter baumannii strain A118	40	90.786	0.363
pilT	Acinetobacter nosocomialis M2	39.701	90.786	0.36