Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   M5C73_RS11645 Genome accession   NZ_CP098790
Coordinates   2330892..2331830 (-) Length   312 a.a.
NCBI ID   WP_058203709.1    Uniprot ID   -
Organism   Lactococcus lactis strain WiKim0124     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2325892..2336830
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M5C73_RS11605 (M5C73_11605) - 2326780..2327622 (-) 843 WP_015427160.1 metal ABC transporter substrate-binding protein -
  M5C73_RS11610 (M5C73_11610) - 2327619..2328056 (-) 438 WP_010906313.1 zinc-dependent MarR family transcriptional regulator -
  M5C73_RS11615 (M5C73_11615) comGG 2328137..2328421 (-) 285 WP_017865155.1 competence type IV pilus minor pilin ComGG Machinery gene
  M5C73_RS11620 (M5C73_11620) comGF 2328460..2328885 (-) 426 WP_161935022.1 competence type IV pilus minor pilin ComGF Machinery gene
  M5C73_RS11625 (M5C73_11625) comGE 2328869..2329165 (-) 297 WP_250354455.1 competence type IV pilus minor pilin ComGE Machinery gene
  M5C73_RS11630 (M5C73_11630) comGD 2329137..2329553 (-) 417 WP_032941843.1 competence type IV pilus minor pilin ComGD Machinery gene
  M5C73_RS11635 (M5C73_11635) comGC 2329528..2329911 (-) 384 WP_080735951.1 competence type IV pilus major pilin ComGC Machinery gene
  M5C73_RS11640 (M5C73_11640) comGB 2329925..2330944 (-) 1020 WP_047206974.1 competence type IV pilus assembly protein ComGB Machinery gene
  M5C73_RS11645 (M5C73_11645) comGA 2330892..2331830 (-) 939 WP_058203709.1 competence type IV pilus ATPase ComGA Machinery gene

Sequence

Protein


Download         Length: 312 a.a.        Molecular weight: 35690.98 Da        Isoelectric Point: 6.6176

>NTDB_id=697996 M5C73_RS11645 WP_058203709.1 2330892..2331830(-) (comGA) [Lactococcus lactis strain WiKim0124]
MIPKMAKELIQRAIDRDASDIYLIASKEKYKLYFRQMTARVLVEEIGLEVGLALLTHFKFLAGMNTGERRRVQLGACWYE
LEESNAKRLRLSTVGDFEGNESLVIRLLHDQNKKLDFWFDEELENYDCKRGLHLFAGPVGSGKTSLMFDLARRHFTDLQV
ITIEEPVELIDSDFIQLQVNEVIGNSYDELIKLSLRHRPDLLIVGEIRDKLTAQAVLRASLTGYTVFSTVHASSVRGVIQ
RLSELGLSHWEIKNALSSVVYQRLIAGKGVLDIAKNEFESWSSENWNKKIDQLLTNGYLTPTEAKREKIKTD

Nucleotide


Download         Length: 939 bp        

>NTDB_id=697996 M5C73_RS11645 WP_058203709.1 2330892..2331830(-) (comGA) [Lactococcus lactis strain WiKim0124]
ATGATACCAAAAATGGCAAAAGAATTAATTCAAAGAGCAATTGATAGAGATGCATCAGATATTTATCTGATTGCTTCAAA
AGAAAAGTATAAACTTTATTTTCGACAAATGACAGCGCGTGTTCTGGTAGAAGAAATTGGGTTAGAAGTTGGCTTAGCAC
TACTTACTCACTTTAAGTTTCTAGCTGGGATGAATACAGGAGAACGCAGACGAGTTCAATTGGGAGCTTGTTGGTATGAA
TTAGAGGAAAGTAATGCAAAACGTTTGCGTCTTTCGACAGTGGGGGATTTCGAGGGTAATGAATCTTTAGTAATACGTCT
CTTGCATGATCAAAACAAAAAACTTGATTTTTGGTTTGATGAAGAGCTAGAGAATTATGATTGTAAAAGAGGATTGCATC
TCTTTGCCGGTCCAGTAGGCTCGGGCAAAACATCTTTAATGTTTGATTTAGCTCGTCGTCATTTTACAGATTTACAAGTT
ATCACTATTGAAGAACCCGTTGAGTTGATTGATTCCGATTTTATTCAGTTACAAGTCAACGAAGTCATTGGTAATAGTTA
TGATGAATTAATTAAATTATCACTGAGGCATCGCCCAGATTTGTTAATCGTTGGTGAAATTCGTGATAAATTGACCGCTC
AAGCAGTTTTACGGGCTAGTTTGACAGGTTATACAGTGTTTTCAACCGTTCATGCCTCTTCTGTTAGAGGTGTCATTCAG
AGATTATCAGAGCTGGGTTTAAGTCATTGGGAAATAAAAAATGCTTTATCAAGTGTTGTTTATCAGCGTTTAATAGCTGG
GAAAGGAGTATTAGACATTGCCAAAAACGAATTTGAGTCTTGGTCATCAGAAAATTGGAATAAAAAAATTGATCAGCTAC
TTACAAATGGATATCTCACACCTACTGAAGCCAAAAGGGAAAAAATTAAGACTGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(9-265)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Lactococcus lactis subsp. cremoris KW2

76.923

100

0.769
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

54.662

99.679

0.545
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

53.376

99.679

0.532
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae D39

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae R6

52.412

99.679

0.522
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

52.412

99.679

0.522
  comYA Streptococcus mutans UA140

52.244

100

0.522
  comYA Streptococcus mutans UA159

52.244

100

0.522
  comYA Streptococcus gordonii str. Challis substr. CH1

51.438

100

0.516