Detailed information NTG0697991

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: M5C73_RS11620
• Genome accession: NZ_CP098790
• Coordinates: 2328460..2328885 (-)
• Length: 141 a.a.
• NCBI ID: WP_161935022.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain WiKim0124
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2323460..2333885

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M5C73_RS11585 (M5C73_11585)	-	2323579..2323956 (-)	378	WP_033900715.1	pyridoxamine 5'-phosphate oxidase family protein	-
M5C73_RS11590 (M5C73_11590)	-	2324160..2325026 (+)	867	WP_058204413.1	RluA family pseudouridine synthase	-
M5C73_RS11595 (M5C73_11595)	-	2325064..2325873 (-)	810	WP_014570791.1	metal ABC transporter permease	-
M5C73_RS11600 (M5C73_11600)	-	2325866..2326603 (-)	738	WP_012898617.1	metal ABC transporter ATP-binding protein	-
M5C73_RS11605 (M5C73_11605)	-	2326780..2327622 (-)	843	WP_015427160.1	metal ABC transporter substrate-binding protein	-
M5C73_RS11610 (M5C73_11610)	-	2327619..2328056 (-)	438	WP_010906313.1	zinc-dependent MarR family transcriptional regulator	-
M5C73_RS11615 (M5C73_11615)	comGG	2328137..2328421 (-)	285	WP_017865155.1	competence type IV pilus minor pilin ComGG	Machinery gene
M5C73_RS11620 (M5C73_11620)	comGF	2328460..2328885 (-)	426	WP_161935022.1	competence type IV pilus minor pilin ComGF	Machinery gene
M5C73_RS11625 (M5C73_11625)	comGE	2328869..2329165 (-)	297	WP_250354455.1	competence type IV pilus minor pilin ComGE	Machinery gene
M5C73_RS11630 (M5C73_11630)	comGD	2329137..2329553 (-)	417	WP_032941843.1	competence type IV pilus minor pilin ComGD	Machinery gene
M5C73_RS11635 (M5C73_11635)	comGC	2329528..2329911 (-)	384	WP_080735951.1	competence type IV pilus major pilin ComGC	Machinery gene
M5C73_RS11640 (M5C73_11640)	comGB	2329925..2330944 (-)	1020	WP_047206974.1	competence type IV pilus assembly protein ComGB	Machinery gene
M5C73_RS11645 (M5C73_11645)	comGA	2330892..2331830 (-)	939	WP_058203709.1	competence type IV pilus ATPase ComGA	Machinery gene

Sequence

Protein

• Length: 141 a.a.
• Molecular weight: 16323.98 Da
• Isoelectric Point: 8.9981

>NTDB_id=697991 M5C73_RS11620 WP_161935022.1 2328460..2328885(-) (comGF) [Lactococcus lactis strain WiKim0124]
MNFKVKAFTLLECLVALLAISGSVLVISGLTKMLKEQVAISQSDSIKDWQIFCQQMRFELSGTKLDRVEQDFLYVTKDKK
LRFGFMRDDFRKTDDKGQGYQPMLYDIKAAKIQATKNLITIRIDFNQGGERTFIYQFPEDT

Nucleotide

• Length: 426 bp

>NTDB_id=697991 M5C73_RS11620 WP_161935022.1 2328460..2328885(-) (comGF) [Lactococcus lactis strain WiKim0124]
TTGAACTTCAAAGTTAAAGCATTTACCTTGTTGGAGTGTTTGGTGGCACTTCTTGCAATTTCTGGTTCAGTCCTTGTCAT
CTCAGGTTTAACAAAGATGTTGAAAGAACAAGTGGCGATTAGTCAAAGTGATAGCATAAAAGATTGGCAAATTTTCTGTC
AGCAAATGCGTTTTGAACTCTCAGGAACAAAATTAGATAGGGTAGAGCAGGATTTTCTGTATGTAACTAAAGACAAAAAA
CTAAGGTTTGGTTTTATGAGAGATGATTTCCGGAAGACTGATGACAAAGGTCAGGGTTATCAGCCAATGCTTTATGATAT
AAAAGCGGCTAAGATTCAAGCTACTAAAAATTTAATAACCATAAGAATTGATTTTAATCAAGGAGGTGAGAGAACATTTA
TTTATCAATTTCCGGAAGATACGTAA

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (41-130)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Lactococcus lactis subsp. cremoris KW2	76.259	98.582	0.752
comYF	Streptococcus mutans UA140	47.826	97.872	0.468
comYF	Streptococcus mutans UA159	47.101	97.872	0.461
comGF/cglF	Streptococcus mitis NCTC 12261	41.606	97.163	0.404
comGF/cglF	Streptococcus mitis SK321	41.606	97.163	0.404
comGF/cglF	Streptococcus pneumoniae Rx1	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae D39	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae R6	39.007	100	0.39
comGF/cglF	Streptococcus pneumoniae TIGR4	39.007	100	0.39